PMID- 29466404
OWN - NLM
STAT- MEDLINE
DCOM- 20180426
LR  - 20240315
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 2
DP  - 2018
TI  - Antioxidant genes of plants and fungal pathogens are distinctly regulated during 
      disease development in different Rhizoctonia solani pathosystems.
PG  - e0192682
LID - 10.1371/journal.pone.0192682 [doi]
LID - e0192682
AB  - Biotic stress, as a result of plant-pathogen interactions, induces the 
      accumulation of reactive oxygen species in the cells, causing severe oxidative 
      damage to plants and pathogens. To overcome this damage, both the host and 
      pathogen have developed antioxidant systems to quench excess ROS and keep ROS 
      production and scavenging systems under control. Data on ROS-scavenging systems 
      in the necrotrophic plant pathogen Rhizoctonia solani are just emerging. We 
      formerly identified vitamin B6 biosynthetic machinery of R. solani AG3 as a 
      powerful antioxidant exhibiting a high ability to quench ROS, similar to CATALASE 
      (CAT) and GLUTATHIONE S-TRANSFERASE (GST). Here, we provide evidence on the 
      involvement of R. solani vitamin B6 biosynthetic pathway genes; RsolPDX1 
      (KF620111.1), RsolPDX2 (KF620112.1), and RsolPLR (KJ395592.1) in vitamin B6 de 
      novo biosynthesis by yeast complementation assays. Since gene expression studies 
      focusing on oxidative stress responses of both the plant and the pathogen 
      following R. solani infection are very limited, this study is the first 
      coexpression analysis of genes encoding vitamin B6, CAT and GST in plant and 
      fungal tissues of three pathosystems during interaction of different AG groups of 
      R. solani with their respective hosts. The findings indicate that distinct 
      expression patterns of fungal and host antioxidant genes were correlated in 
      necrotic tissues and their surrounding areas in each of the three R. solani 
      pathosystems: potato sprout-R. solani AG3; soybean hypocotyl-R. solani AG4 and 
      soybean leaves-R. solani AG1-IA interactions. Levels of ROS increased in all 
      types of potato and soybean tissues, and in fungal hyphae following infection of 
      R. solani AGs as determined by non-fluorescence and fluorescence methods using 
      H2DCF-DA and DAB, respectively. Overall, we demonstrate that the co-expression 
      and accumulation of certain plant and pathogen ROS-antioxidant related genes in 
      each pathosystem are highlighted and might be critical during disease development 
      from the plant's point of view, and in pathogenicity and developing of infection 
      structures from the fungal point of view.
FAU - Samsatly, Jamil
AU  - Samsatly J
AD  - Plant Science Department, Macdonald Campus, McGill University, 
      Ste-Anne-de-Bellevue, Canada.
FAU - Copley, Tanya R
AU  - Copley TR
AD  - Plant Science Department, Macdonald Campus, McGill University, 
      Ste-Anne-de-Bellevue, Canada.
FAU - Jabaji, Suha H
AU  - Jabaji SH
AUID- ORCID: 0000-0002-3050-4723
AD  - Plant Science Department, Macdonald Campus, McGill University, 
      Ste-Anne-de-Bellevue, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180221
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antioxidants)
SB  - IM
MH  - Antioxidants/*metabolism
MH  - Fungi/*genetics
MH  - Genes, Fungal
MH  - Genes, Plant
MH  - Genetic Complementation Test
MH  - Plant Diseases/*microbiology
MH  - Plants/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Rhizoctonia/*metabolism
PMC - PMC5821333
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2018/02/22 06:00
MHDA- 2018/04/27 06:00
PMCR- 2018/02/21
CRDT- 2018/02/22 06:00
PHST- 2017/08/24 00:00 [received]
PHST- 2018/01/29 00:00 [accepted]
PHST- 2018/02/22 06:00 [entrez]
PHST- 2018/02/22 06:00 [pubmed]
PHST- 2018/04/27 06:00 [medline]
PHST- 2018/02/21 00:00 [pmc-release]
AID - PONE-D-17-29567 [pii]
AID - 10.1371/journal.pone.0192682 [doi]
PST - epublish
SO  - PLoS One. 2018 Feb 21;13(2):e0192682. doi: 10.1371/journal.pone.0192682. 
      eCollection 2018.