PMID- 29467621
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 12
DP  - 2018
TI  - Immunosignals of Oligodendrocyte Markers and Myelin-Associated Proteins Are 
      Critically Affected after Experimental Stroke in Wild-Type and Alzheimer Modeling 
      Mice of Different Ages.
PG  - 23
LID - 10.3389/fncel.2018.00023 [doi]
LID - 23
AB  - Because stroke therapies are still limited and patients are often concerned by 
      long-term sequelae with significant impairment of daily living, elaborated 
      neuroprotective strategies are needed. During the last decades, research 
      substantially improved the knowledge on cellular pathologies responsible for 
      stroke-related tissue damage. In this context, the neurovascular unit (NVU) 
      concept has been established, summarizing the affections of neurons, associated 
      astrocytes and the vasculature. Although oligodendrocytes were already identified 
      to play a major role in other brain pathologies, their role during stroke 
      evolution and long-lasting tissue damage is poorly understood. This study aims to 
      explore oligodendrocyte structures, i.e., oligodendrocytes and their 
      myelin-associated proteins, after experimental focal cerebral ischemia. For 
      translational issues, different ages and genotypes including an Alzheimer-like 
      background were considered to mimic potential co-morbidities. Three- and 
      12-month-old wild-type and triple-transgenic mice were subjected to unilateral 
      middle cerebral artery occlusion. Immunofluorescence labeling was performed on 
      forebrain tissues affected by 24 h of ischemia to visualize the 
      oligodendrocyte-specific protein (OSP), the myelin basic protein (MBP), and the 
      neuron-glia antigen 2 (NG2) with reference to the ischemic lesion. Subsequent 
      analyses concomitantly detected the vasculature and the 2', 3'-cyclic 
      nucleotide-3'-phosphodiesterase (CNPase) to consider the NVU concept and to 
      explore the functional relevance of histochemical data on applied oligodendrocyte 
      markers. While the immunosignal of NG2 was found to be nearly absent 24 h after 
      ischemia onset, enhanced immunoreactivities for OSP and especially MBP were 
      observed in close regional association to the vasculature. Added quantitative 
      analyses based on inter-hemispheric differences of MBP-immunoreactivity revealed 
      a shell-like pattern with a significant increase directly in the ischemic core, 
      followed by a gradual decline toward the striatum, the ischemic border zone and 
      the lateral neocortex. This observation was consistent in subsequent analyses on 
      the potential impact of age and genetic background. Furthermore, 
      immunoreactivities for CNPase, MBP, and OSP were found to be simultaneously 
      enhanced. In conclusion, this study provides evidence for a critical role of 
      oligodendrocyte structures in the early phase after experimental stroke, 
      strengthening their involvement in the ischemia-affected NVU. Consequently, 
      oligodendrocytes and their myelin-associated proteins may qualify as potential 
      targets for neuroprotective and regenerative approaches in stroke.
FAU - Michalski, Dominik
AU  - Michalski D
AD  - Department of Neurology, University of Leipzig, Leipzig, Germany.
FAU - Keck, Anna L
AU  - Keck AL
AD  - Paul Flechsig Institute for Brain Research, University of Leipzig, Leipzig, 
      Germany.
FAU - Grosche, Jens
AU  - Grosche J
AD  - Effigos GmbH, Leipzig, Germany.
FAU - Martens, Henrik
AU  - Martens H
AD  - Synaptic Systems GmbH, Gottingen, Germany.
FAU - Hartig, Wolfgang
AU  - Hartig W
AD  - Paul Flechsig Institute for Brain Research, University of Leipzig, Leipzig, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20180206
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC5807905
OTO - NOTNLM
OT  - 3xTg mouse
OT  - animal model
OT  - cerebral ischemia
OT  - myelin basic protein
OT  - oligodendrocyte
OT  - oligodendrocyte progenitor cells
OT  - stroke
EDAT- 2018/02/23 06:00
MHDA- 2018/02/23 06:01
PMCR- 2018/01/01
CRDT- 2018/02/23 06:00
PHST- 2017/09/24 00:00 [received]
PHST- 2018/01/15 00:00 [accepted]
PHST- 2018/02/23 06:00 [entrez]
PHST- 2018/02/23 06:00 [pubmed]
PHST- 2018/02/23 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2018.00023 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2018 Feb 6;12:23. doi: 10.3389/fncel.2018.00023. eCollection 
      2018.