PMID- 29467869 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220321 IS - 1792-1074 (Print) IS - 1792-1082 (Electronic) IS - 1792-1074 (Linking) VI - 15 IP - 3 DP - 2018 Mar TI - Role of epithelial-mesenchymal transition markers E-cadherin, N-cadherin, beta-catenin and ZEB2 in laryngeal squamous cell carcinoma. PG - 3472-3481 LID - 10.3892/ol.2018.7751 [doi] AB - Epithelial-mesenchymal transition (EMT) allows neoplastic cells to gain the invasive phenotype and become migratory, which is required for cancer progression and metastasis. In the present study, the expression of EMT-associated biomarkers and their association with clinicopathological parameters in laryngeal squamous cell carcinoma (LSCC) was investigated. E-cadherin, N-cadherin, beta-catenin and zinc finger E-box binding homeobox 2 (ZEB2) protein expression was evaluated with immunohistochemistry in a cohort of 76 patients with operable LSCC. The association between these transition markers, clinicopathological parameters and their prognostic impact in LSCC was analyzed. Immunohistochemical analysis revealed that EMT-associated proteins were differentially expressed between LSCC and adjacent non-neoplastic laryngeal tissue. Negative E-cadherin expression and positive N-cadherin, beta-catenin and ZEB2 expression were associated with a later tumor (T) stage, decreasing tumor differentiation and a reduced overall survival (OS) time (OS: E-cadherin, P=0.016; N-cadherin, P=0.003; beta-catenin, P=0.002; ZEB2, P=0.0003). E-cadherin/beta-catenin co-expression was significantly associated with the majority of clinicopathological parameters assessed, including lymph node metastases, T stage and tumor cell differentiation (P=0.004, P=0.005, and P<0.001, respectively). Multivariate analysis indicated that T stage and the positive expression of beta-catenin and ZEB2 were independent risk factors for OS in LSCC (P=0.014, P=0.025 and P=0.003, respectively). It was concluded that EMT mediates tumor progression, and reduces OS time in patients with LSCC. E-cadherin/beta-catenin co-expression may be associated with clinicopathological parameters. T stage, and the positive co-expression of beta-catenin and ZEB2 may be independent predictors of prognosis in LSCC. FAU - Zhu, Guang-Jie AU - Zhu GJ AD - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, P.R. China. AD - Department of Research Institution of Otolaryngology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China. FAU - Song, Pan-Pan AU - Song PP AD - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, P.R. China. AD - Department of Research Institution of Otolaryngology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China. FAU - Zhou, Han AU - Zhou H AD - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, P.R. China. AD - Department of Research Institution of Otolaryngology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China. FAU - Shen, Xiao-Hui AU - Shen XH AD - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, P.R. China. AD - Department of Research Institution of Otolaryngology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China. FAU - Wang, Jun-Guo AU - Wang JG AD - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, P.R. China. AD - Department of Research Institution of Otolaryngology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China. FAU - Ma, Xiao-Feng AU - Ma XF AD - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, P.R. China. AD - Department of Research Institution of Otolaryngology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China. FAU - Gu, Ya-Jun AU - Gu YJ AD - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, P.R. China. AD - Department of Research Institution of Otolaryngology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China. FAU - Liu, Ding-Ding AU - Liu DD AD - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, P.R. China. AD - Department of Research Institution of Otolaryngology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China. FAU - Feng, An-Ning AU - Feng AN AD - Department of Pathology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China. FAU - Qian, Xiao-Yun AU - Qian XY AD - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, P.R. China. AD - Department of Research Institution of Otolaryngology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China. FAU - Gao, Xia AU - Gao X AD - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, Jiangsu 210008, P.R. China. AD - Department of Research Institution of Otolaryngology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China. LA - eng PT - Journal Article DEP - 20180108 PL - Greece TA - Oncol Lett JT - Oncology letters JID - 101531236 PMC - PMC5796309 OTO - NOTNLM OT - epithelial-mesenchymal transition OT - laryngeal carcinoma OT - prognosis EDAT- 2018/02/23 06:00 MHDA- 2018/02/23 06:01 PMCR- 2018/01/08 CRDT- 2018/02/23 06:00 PHST- 2016/12/07 00:00 [received] PHST- 2017/10/13 00:00 [accepted] PHST- 2018/02/23 06:00 [entrez] PHST- 2018/02/23 06:00 [pubmed] PHST- 2018/02/23 06:01 [medline] PHST- 2018/01/08 00:00 [pmc-release] AID - OL-0-0-7751 [pii] AID - 10.3892/ol.2018.7751 [doi] PST - ppublish SO - Oncol Lett. 2018 Mar;15(3):3472-3481. doi: 10.3892/ol.2018.7751. Epub 2018 Jan 8.