PMID- 29468305
OWN - NLM
STAT- MEDLINE
DCOM- 20180920
LR  - 20181113
IS  - 1432-0991 (Electronic)
IS  - 0343-8651 (Linking)
VI  - 75
IP  - 7
DP  - 2018 Jul
TI  - Transovarian Transmission of Blochmannia and Wolbachia Endosymbionts in the 
      Neotropical Weaver Ant Camponotus textor (Hymenoptera, Formicidae).
PG  - 866-873
LID - 10.1007/s00284-018-1459-3 [doi]
AB  - Camponotus is a hyper-diverse ant genus that is associated with the obligate 
      endosymbiont Blochmannia, and often also with Wolbachia, but morphological 
      studies on the location of these bacteria in the queen's ovaries during oogenesis 
      remain limited. In the present study, we used the Neotropical weaver ant 
      Camponotus textor to characterize the ovary using histology (HE) techniques, and 
      to document the location of Blochmannia and Wolbachia during oogenesis through 
      fluorescence in situ hybridization (FISH). This is the first morphological report 
      of these two bacteria in the same host with polytrophic meroistic ovaries and 
      reveals that Blochmannia is found inside late-stage oocytes and Wolbachia is 
      associated with the nuclei of the nurse cells. Our results provide insights into 
      the developmental sequence of when these bacteria reach the egg, with Blochmannia 
      establishing itself in the egg first, and Wolbachia only reaching the egg shortly 
      before completing egg development. Studies such as this provide understanding 
      about the mechanisms and timing of the establishment of these endosymbionts in 
      the host.
FAU - Ramalho, Manuela Oliveira
AU  - Ramalho MO
AUID- ORCID: 0000-0002-8144-6172
AD  - Departament of Biology e Center for Studies on Social Insects, Biosciense 
      Institute, Sao Paulo State University (UNESP), Campus Rio Claro, Avenida 24A, 
      1515, Bela Vista, Rio Claro, SP, 13506-900, Brazil. manuramalho2010@gmail.com.
AD  - Field Museum of Natural History, Department of Science and Education, Integrative 
      Research Center, 1400 South Lake Shore Drive, Chicago, IL, 60605, USA. 
      manuramalho2010@gmail.com.
FAU - Vieira, Alexsandro Santana
AU  - Vieira AS
AD  - Departament of Biology e Center for Studies on Social Insects, Biosciense 
      Institute, Sao Paulo State University (UNESP), Campus Rio Claro, Avenida 24A, 
      1515, Bela Vista, Rio Claro, SP, 13506-900, Brazil.
FAU - Pereira, Mayara Cristina
AU  - Pereira MC
AD  - Departament of Biology e Center for Studies on Social Insects, Biosciense 
      Institute, Sao Paulo State University (UNESP), Campus Rio Claro, Avenida 24A, 
      1515, Bela Vista, Rio Claro, SP, 13506-900, Brazil.
FAU - Moreau, Corrie Saux
AU  - Moreau CS
AD  - Field Museum of Natural History, Department of Science and Education, Integrative 
      Research Center, 1400 South Lake Shore Drive, Chicago, IL, 60605, USA.
FAU - Bueno, Odair Correa
AU  - Bueno OC
AD  - Departament of Biology e Center for Studies on Social Insects, Biosciense 
      Institute, Sao Paulo State University (UNESP), Campus Rio Claro, Avenida 24A, 
      1515, Bela Vista, Rio Claro, SP, 13506-900, Brazil.
LA  - eng
GR  - 007343/2014-00/CAPES Foundation/
GR  - 157837/2015-7/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
PT  - Journal Article
DEP - 20180221
PL  - United States
TA  - Curr Microbiol
JT  - Current microbiology
JID - 7808448
SB  - IM
MH  - Animals
MH  - Ants/growth & development/*microbiology
MH  - Enterobacteriaceae/classification/genetics/*isolation & purification/physiology
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Ovary/growth & development/microbiology
MH  - Ovum/growth & development/microbiology
MH  - *Symbiosis
MH  - Wolbachia/classification/genetics/*isolation & purification/physiology
EDAT- 2018/02/23 06:00
MHDA- 2018/09/21 06:00
CRDT- 2018/02/23 06:00
PHST- 2017/12/07 00:00 [received]
PHST- 2018/02/19 00:00 [accepted]
PHST- 2018/02/23 06:00 [pubmed]
PHST- 2018/09/21 06:00 [medline]
PHST- 2018/02/23 06:00 [entrez]
AID - 10.1007/s00284-018-1459-3 [pii]
AID - 10.1007/s00284-018-1459-3 [doi]
PST - ppublish
SO  - Curr Microbiol. 2018 Jul;75(7):866-873. doi: 10.1007/s00284-018-1459-3. Epub 2018 
      Feb 21.