PMID- 29468547
OWN - NLM
STAT- MEDLINE
DCOM- 20190115
LR  - 20190115
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1726
DP  - 2018
TI  - Detection of CCND1 Gene Copy Number Variations Using Multiplex Ligation-Dependent 
      Probe Amplification and Fluorescence In Situ Hybridization Methods.
PG  - 101-109
LID - 10.1007/978-1-4939-7565-5_10 [doi]
AB  - The CCND1 locus is located in 11q13 and encodes the G1-S regulatory protein, 
      cyclin D1. Cyclin D1 is frequently amplified in various types of cancers, and is 
      an attractive potential therapeutic target. Multiplex ligation-dependent probe 
      amplification (MLPA) is a new, high-resolution method for the detection of 
      amplification of numerous genes including CCND1 in small amounts of DNA fragments 
      derived from formalin-fixed, paraffin-embedded material in a single reaction. 
      This approach is, however, based on PCR and averages many different cells, so 
      validation by morphological methods such as fluorescence in situ hybridization 
      (FISH) is theoretically mandatory. Here we describe detection of CCND1 gene copy 
      number variations by commercially available MLPA kits and FISH using a bacterial 
      artificial chromosome (BAC) probe.
FAU - Ooi, Akishi
AU  - Ooi A
AD  - Department of Molecular and Cellular Pathology, Graduate School of Medical 
      Science, Kanazawa University, Kanazawa, Ishikawa, Japan. 
      aooi@med.kanazawa-uac.jp.
FAU - Oyama, Takeru
AU  - Oyama T
AD  - Department of Molecular and Cellular Pathology, Graduate School of Medical 
      Science, Kanazawa University, Kanazawa, Ishikawa, Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (CCND1 protein, human)
RN  - 136601-57-5 (Cyclin D1)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cyclin D1/*genetics
MH  - DNA/analysis
MH  - *DNA Copy Number Variations
MH  - Fluorescence
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Multiplex Polymerase Chain Reaction/*methods
MH  - Neoplasms/*diagnosis/genetics
MH  - Point Mutation
OTO - NOTNLM
OT  - BAC probe
OT  - Commercially available MLPA kits
OT  - Formalin-fixed, paraffin-embedded material
EDAT- 2018/02/23 06:00
MHDA- 2019/01/16 06:00
CRDT- 2018/02/23 06:00
PHST- 2018/02/23 06:00 [entrez]
PHST- 2018/02/23 06:00 [pubmed]
PHST- 2019/01/16 06:00 [medline]
AID - 10.1007/978-1-4939-7565-5_10 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1726:101-109. doi: 10.1007/978-1-4939-7565-5_10.