PMID- 29468755
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1439-0272 (Electronic)
IS  - 0303-4569 (Linking)
DP  - 2018 Feb 22
TI  - Impact of a mild scrotal heating on sperm chromosomal abnormality, acrosin 
      activity and seminal alpha-glucosidase in human fertile males.
LID - 10.1111/and.12985 [doi]
AB  - The aim of this study was to observe sperm aneuploidy, DNA integrity, seminal 
      alpha-glucosidase (NAG) and acrosin activity (AA) under testicular heat stress 
      (SH). Spermatozoa were obtained from 30 healthy adult volunteers subjected to 
      scrotal warming at 43 degrees C for 30-40 min on two successive days per week for 
      3 months between February 2012 and September 2016. Aniline blue (AB), acridine 
      orange (AO) staining, TUNEL assay and FISH analysis to evaluate sperm function, 
      sperm DNA integrity and chromosomal abnormalities were carried on before, during 
      and after SH. Sperm AA and NAG was measured by microplate reader. The mean 
      parameters of sperm parameters, AA and NAG were significantly decreased. In 
      contrast, the mean percentage of sperm DNA fragmentation and the proportion of 
      aneuploidy of chromosomes 13, 18, 21, X and Y were significantly increased for 
      spermatozoa collected during SH versus before SH (p < .01-.001). After stopping 
      scrotal heating for 3 months, most parameters were completely restored to pre-SH 
      levels. Sperm parameters, sperm DNA integrity, chromosomes, AA and NAG are 
      affected by scrotal exposure to constant SH temperatures several degrees over 
      normal physiological temperature, and after treatment, these parameters were 
      reversibly restored to the level before SH in adult men.
CI  - (c) 2018 Blackwell Verlag GmbH.
FAU - Zhang, M-H
AU  - Zhang MH
AUID- ORCID: 0000-0002-6821-1168
AD  - Key Laboratory of Birth Regulation and Control Technology of National Health and 
      Family Planning Commission of China, Key Laboratory for Improving Birth Outcome 
      Technique, Shandong Provincial Family Planning Institute of Science and 
      Technology, Maternal and Child Health Care Hospital of Shandong Province, Jinan, 
      China.
FAU - Zhai, L-P
AU  - Zhai LP
AD  - Shandong Provincial Institute of Control of Endemic Disease, Jinan, China.
FAU - Fang, Z-Y
AU  - Fang ZY
AD  - Key Laboratory of Birth Regulation and Control Technology of National Health and 
      Family Planning Commission of China, Key Laboratory for Improving Birth Outcome 
      Technique, Shandong Provincial Family Planning Institute of Science and 
      Technology, Maternal and Child Health Care Hospital of Shandong Province, Jinan, 
      China.
FAU - Li, A-N
AU  - Li AN
AD  - Key Laboratory of Birth Regulation and Control Technology of National Health and 
      Family Planning Commission of China, Key Laboratory for Improving Birth Outcome 
      Technique, Shandong Provincial Family Planning Institute of Science and 
      Technology, Maternal and Child Health Care Hospital of Shandong Province, Jinan, 
      China.
FAU - Qiu, Y
AU  - Qiu Y
AD  - Key Laboratory of Birth Regulation and Control Technology of National Health and 
      Family Planning Commission of China, Key Laboratory for Improving Birth Outcome 
      Technique, Shandong Provincial Family Planning Institute of Science and 
      Technology, Maternal and Child Health Care Hospital of Shandong Province, Jinan, 
      China.
FAU - Liu, Y-X
AU  - Liu YX
AD  - Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20180222
PL  - Germany
TA  - Andrologia
JT  - Andrologia
JID - 0423506
OTO - NOTNLM
OT  - acrosin activity
OT  - alpha-glucosidase
OT  - fluorescence in situ hybridisation
OT  - scrotal heating
OT  - sperm chromosomes
EDAT- 2018/02/23 06:00
MHDA- 2018/02/23 06:00
CRDT- 2018/02/23 06:00
PHST- 2018/01/08 00:00 [accepted]
PHST- 2018/02/23 06:00 [entrez]
PHST- 2018/02/23 06:00 [pubmed]
PHST- 2018/02/23 06:00 [medline]
AID - 10.1111/and.12985 [doi]
PST - aheadofprint
SO  - Andrologia. 2018 Feb 22. doi: 10.1111/and.12985.