PMID- 29469044
OWN - NLM
STAT- MEDLINE
DCOM- 20190422
LR  - 20210928
IS  - 1665-2681 (Print)
IS  - 1665-2681 (Linking)
VI  - 17
IP  - 2
DP  - 2018 Mar 1
TI  - TCR Vbeta Usage of Peripheral Blood and Liver Infiltrating Lymphocytes in Patients 
      with Chronic Hepatitis B.
PG  - 214-222
LID - 10.5604/01.3001.0010.8636 [doi]
AB  - INTRODUCTION: Chronic hepatitis B (CHB) is still a public health problem and its 
      mechanism remains unclear. In this study, we detect the skewness of T cell 
      receptor beta chain variable gene (TCR Vbeta) in peripheral blood lymphocytes (PBL) 
      and the liver infiltrating lymphocytes (LIL) of patients with CHB; and hope to 
      provide information for further research on the pathogenic mechanism of CHB. 
      MATERIAL AND METHODS: Fifteen patients with CHB, ten healthy volunteers and three 
      patients with liver cysts were recruited as the subjects. The usage of TCR Vbeta of 
      PBL and LIL were measured and compared; the associations of the TCR Vbeta usage of 
      PBL with some hematological indices, including human leukocyte antigen (HLA) 
      alleles, percents of CD4+ and CD8+ T cells, sera levels of HBV-DNA and IFN-gamma, 
      were analyzed. RESULTS: In PBL, Vbeta12 and Vbeta13.1 were the highest predominant 
      usage genes which usage frequencies were all 46.7%; Vbeta23 was the key limited 
      usage gene (40.0%). In LIL, the mainly predominant and limited usage gene was 
      Vbeta13.1 (73.3%) and Vbeta23 (46.7%), respectively. About half of the patients with 
      CHB with HLA-DR9 or HLA-DR12 showed the predominant usage of Vbeta5.2 or Vbeta13.2. In 
      patients with CHB, the percentage of CD4+ T cells was 33.41 +/- 5.39 %, that of 
      CD8+ T cells was 28.67 +/- 6.77 %; the concentration of IFN-gamma was 182.52 +/- 44.16 
      pg/mL. Compared to the healthy controls, there were significant differences for 
      these data (P < 0.05). Neither ALT nor HBV-DNA was relative to the usage of 
      TCR Vbeta. CONCLUSIONS: PBL and LIL share the common sknewness of TCR Vbeta genes, 
      which probably relates to some hematological indices. However, the roles of such 
      similarities and associations in the development of CHB need further study.
FAU - Zhou, Jianwei
AU  - Zhou J
AD  - Affiliated Hospital of Jining Medical University, China. Clinical Laboratory.
FAU - Kong, Cui
AU  - Kong C
AD  - Affiliated Hospital of Jining Medical University, China. Nursing Department.
FAU - Ban, Bo
AU  - Ban B
AD  - Affiliated Hospital of Jining Medical University, China. Endocrinology 
      Department.
FAU - Dong, Haixin
AU  - Dong H
AD  - Affiliated Hospital of Jining Medical University, China. Clinical Laboratory.
FAU - Jin, Chengqiang
AU  - Jin C
AD  - Affiliated Hospital of Jining Medical University, China. Clinical Laboratory.
LA  - eng
PT  - Journal Article
PL  - Mexico
TA  - Ann Hepatol
JT  - Annals of hepatology
JID - 101155885
RN  - 0 (Complementarity Determining Regions)
RN  - 0 (HLA-DR Serological Subtypes)
RN  - 0 (HLA-DR12 antigen)
RN  - 0 (HLA-DR9 antigen)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Complementarity Determining Regions/genetics/immunology
MH  - Female
MH  - *Genes, T-Cell Receptor beta
MH  - HLA-DR Serological Subtypes/genetics/immunology
MH  - Hepatitis B, Chronic/diagnosis/*genetics/*immunology/virology
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Immunoglobulin Variable Region/*genetics/immunology
MH  - Liver/*immunology/virology
MH  - Lymphocytes/*immunology/virology
MH  - Male
MH  - Middle Aged
MH  - Receptors, Antigen, T-Cell, alpha-beta/*genetics/immunology
OTO - NOTNLM
OT  - Complementarity determining region 3. Human leukocyte antigen. Liver infiltrating 
      lymphocyte. Peripheral blood lymphocyte. T cell receptor.
EDAT- 2018/02/23 06:00
MHDA- 2019/04/23 06:00
CRDT- 2018/02/23 06:00
PHST- 2018/02/23 06:00 [entrez]
PHST- 2018/02/23 06:00 [pubmed]
PHST- 2019/04/23 06:00 [medline]
AID - S1665-2681(19)30153-X [pii]
AID - 10.5604/01.3001.0010.8636 [doi]
PST - ppublish
SO  - Ann Hepatol. 2018 Mar 1;17(2):214-222. doi: 10.5604/01.3001.0010.8636.