PMID- 29470691 OWN - NLM STAT- MEDLINE DCOM- 20190903 LR - 20210109 IS - 1436-6215 (Electronic) IS - 1436-6207 (Linking) VI - 58 IP - 2 DP - 2019 Mar TI - Even effect of milk protein and carbohydrate intake but no further effect of heavy resistance exercise on myofibrillar protein synthesis in older men. PG - 583-595 LID - 10.1007/s00394-018-1641-1 [doi] AB - PURPOSE: The responsiveness of older individuals' skeletal muscle to anabolic strategies may be impaired. However, direct comparisons within the same experimental setting are sparse. The aim of this study was to assess the resting and post-resistance exercise muscle protein synthesis rates in response to two types of milk protein and carbohydrate using a unilateral exercise leg model. METHODS: Twenty-seven older (69 +/- 1 year, mean +/- SE) men were randomly assigned one of three groups: Whey hydrolysate (WH), caseinate (CAS), or carbohydrate (CHO). By applying stable isotope tracer techniques (L-[(15)N]phenylalanine), the fasted-rested (basal) myofibrillar fractional synthesis rate (FSR) was measured. Hereafter, FSR was measured in the postprandial phase (0.45 g nutrient/kg LBM) in both legs, one rested (fed-rest) and one exercised (10 x 8 reps at 70% 1RM; fed-exercise). In addition, the activity of p70S6K and venous plasma insulin, phenylalanine, and leucine concentrations were measured. RESULTS: Insulin, phenylalanine, and leucine concentrations differed markedly after intake of the different study drinks. The basal FSR in WH, CAS, and CHO were 0.027 +/- 0.003, 0.030 +/- 0.003, and 0.030 +/- 0.004%/h, the fed-rested FSR were 0.043 +/- 0.004, 0.045 +/- 0.003, and 0.035 +/- 0.004%/h, and the fed-exercised FSR were 0.041 +/- 0.004, 0.043 +/- 0.004, and 0.034 +/- 0.004%/h, respectively. No significant differences were observed at any state between the groups. Fed-rested- and fed-exercised FSR were higher than basal (P < 0.001). 3 h after exercise and feeding, no significant group differences were detected in the activity of p70S6K. CONCLUSIONS: Milk protein and carbohydrate supplementation stimulate myofibrillar protein synthesis in older men, with no further effect of heavy resistance exercise within 0-3 h post exercise. FAU - Reitelseder, Soren AU - Reitelseder S AD - Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Bispebjerg Bakke 23, building 8, 1st floor, 2400, Copenhagen NV, Denmark. soeren.reitelseder@regionh.dk. FAU - Dideriksen, Kasper AU - Dideriksen K AD - Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Bispebjerg Bakke 23, building 8, 1st floor, 2400, Copenhagen NV, Denmark. FAU - Agergaard, Jakob AU - Agergaard J AD - Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Bispebjerg Bakke 23, building 8, 1st floor, 2400, Copenhagen NV, Denmark. FAU - Malmgaard-Clausen, Nikolaj M AU - Malmgaard-Clausen NM AD - Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Bispebjerg Bakke 23, building 8, 1st floor, 2400, Copenhagen NV, Denmark. FAU - Bechshoeft, Rasmus L AU - Bechshoeft RL AD - Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Bispebjerg Bakke 23, building 8, 1st floor, 2400, Copenhagen NV, Denmark. FAU - Petersen, Rasmus K AU - Petersen RK AD - Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark. FAU - Serena, Anja AU - Serena A AD - Arla Foods amba, Viby J, Denmark. FAU - Mikkelsen, Ulla R AU - Mikkelsen UR AD - Arla Foods Ingredients Group P/S, Viby J, Denmark. FAU - Holm, Lars AU - Holm L AD - Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Bispebjerg Bakke 23, building 8, 1st floor, 2400, Copenhagen NV, Denmark. AD - School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham, UK. LA - eng PT - Journal Article PT - Randomized Controlled Trial DEP - 20180222 PL - Germany TA - Eur J Nutr JT - European journal of nutrition JID - 100888704 RN - 0 (Dietary Carbohydrates) RN - 0 (Milk Proteins) RN - 0 (Muscle Proteins) SB - IM MH - Aged MH - Dietary Carbohydrates/*pharmacology MH - Humans MH - Leg MH - Male MH - Milk Proteins/*pharmacology MH - Muscle Proteins/*biosynthesis MH - *Resistance Training OTO - NOTNLM OT - Anabolic resistance OT - Caseinate OT - Muscle protein OT - Sarcopenia OT - Whey hydrolysate EDAT- 2018/02/23 06:00 MHDA- 2019/09/04 06:00 CRDT- 2018/02/23 06:00 PHST- 2017/08/14 00:00 [received] PHST- 2018/02/17 00:00 [accepted] PHST- 2018/02/23 06:00 [pubmed] PHST- 2019/09/04 06:00 [medline] PHST- 2018/02/23 06:00 [entrez] AID - 10.1007/s00394-018-1641-1 [pii] AID - 10.1007/s00394-018-1641-1 [doi] PST - ppublish SO - Eur J Nutr. 2019 Mar;58(2):583-595. doi: 10.1007/s00394-018-1641-1. Epub 2018 Feb 22.