PMID- 29470990
OWN - NLM
STAT- MEDLINE
DCOM- 20180329
LR  - 20180329
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 497
IP  - 3
DP  - 2018 Mar 11
TI  - Lis1 dysfunction leads to traction force reduction and cytoskeletal 
      disorganization during cell migration.
PG  - 869-875
LID - S0006-291X(18)30388-7 [pii]
LID - 10.1016/j.bbrc.2018.02.151 [doi]
AB  - Cell migration is a critical process during development, tissue repair, and 
      cancer metastasis. It requires complex processes of cell adhesion, cytoskeletal 
      dynamics, and force generation. Lis1 plays an important role in the migration of 
      neurons, fibroblasts and other cell types, and is essential for normal 
      development of the cerebral cortex. Mutations in human LIS1 gene cause classical 
      lissencephaly (smooth brain), resulting from defects in neuronal migration. 
      However, how Lis1 may affect force generation in migrating cells is still not 
      fully understood. Using traction force microscopy (TFM) with live cell imaging to 
      measure cellular traction force in migrating NIH3T3 cells, we showed that Lis1 
      knockdown (KD) by RNA interference (RNAi) caused reductions in cell migration and 
      traction force against the extracellular matrix (ECM). Immunostaining of 
      cytoskeletal components in Lis1 KD cells showed disorganization of microtubules 
      and actin filaments. Interestingly, focal adhesions at the cell periphery were 
      significantly reduced. These results suggest that Lis1 is important for cellular 
      traction force generation through the regulation of cytoskeleton organization and 
      focal adhesion formation in migrating cells.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Jheng, Guo-Wei
AU  - Jheng GW
AD  - Institute of Brain Science, School of Medicine, National Yang-Ming University, 
      Taipei 112, Taiwan, ROC.
FAU - Hur, Sung Sik
AU  - Hur SS
AD  - Department of Bioengineering and Institute of Engineering in Medicine, University 
      of California at San Diego, La Jolla, CA 92093, USA.
FAU - Chang, Chia-Ming
AU  - Chang CM
AD  - Institute of Brain Science, School of Medicine, National Yang-Ming University, 
      Taipei 112, Taiwan, ROC.
FAU - Wu, Chun-Chieh
AU  - Wu CC
AD  - Institute of Brain Science, School of Medicine, National Yang-Ming University, 
      Taipei 112, Taiwan, ROC.
FAU - Cheng, Jia-Shing
AU  - Cheng JS
AD  - Institute of Brain Science, School of Medicine, National Yang-Ming University, 
      Taipei 112, Taiwan, ROC.
FAU - Lee, Hsiao-Hui
AU  - Lee HH
AD  - Department of Life Sciences and Institute of Genome Sciences, School of Life 
      Sciences, National Yang-Ming University, Taipei 112, Taiwan, ROC.
FAU - Chung, Bon-Chu
AU  - Chung BC
AD  - Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan, ROC.
FAU - Wang, Yang-Kao
AU  - Wang YK
AD  - Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung 
      University, Tainan 70101, Taiwan, ROC.
FAU - Lin, Keng-Hui
AU  - Lin KH
AD  - Institute of Physics, Academia Sinica, Taipei 11529, Taiwan, ROC.
FAU - Del Alamo, Juan C
AU  - Del Alamo JC
AD  - Department of Mechanical and Aerospace Engineering, University of California, La 
      Jolla, San Diego, CA 92093, USA.
FAU - Chien, Shu
AU  - Chien S
AD  - Department of Bioengineering and Institute of Engineering in Medicine, University 
      of California at San Diego, La Jolla, CA 92093, USA.
FAU - Tsai, Jin-Wu
AU  - Tsai JW
AD  - Institute of Brain Science, School of Medicine, National Yang-Ming University, 
      Taipei 112, Taiwan, ROC; Brain Research Center (BRC) and Biophotonics and 
      Molecular Imaging Research Center (BMIRC), National Yang-Ming University, Taipei 
      112, Taiwan, ROC. Electronic address: tsaijw@ym.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180220
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Microtubule-Associated Proteins)
RN  - EC 3.1.1.47 (1-Alkyl-2-acetylglycerophosphocholine Esterase)
RN  - EC 3.1.1.47 (Pafah1b1 protein, mouse)
SB  - IM
MH  - 1-Alkyl-2-acetylglycerophosphocholine Esterase/genetics/*metabolism
MH  - Animals
MH  - Biomechanical Phenomena
MH  - *Cell Movement
MH  - Cytoskeleton/*metabolism
MH  - Fibroblasts/*cytology/metabolism
MH  - Focal Adhesions/metabolism
MH  - Mice
MH  - Microtubule-Associated Proteins/genetics/*metabolism
MH  - NIH 3T3 Cells
MH  - RNA Interference
OTO - NOTNLM
OT  - Actin
OT  - Focal adhesion
OT  - Lis1
OT  - Microtubule
OT  - Migration
OT  - Traction force
EDAT- 2018/02/23 06:00
MHDA- 2018/03/30 06:00
CRDT- 2018/02/23 06:00
PHST- 2018/02/05 00:00 [received]
PHST- 2018/02/17 00:00 [accepted]
PHST- 2018/02/23 06:00 [pubmed]
PHST- 2018/03/30 06:00 [medline]
PHST- 2018/02/23 06:00 [entrez]
AID - S0006-291X(18)30388-7 [pii]
AID - 10.1016/j.bbrc.2018.02.151 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Mar 11;497(3):869-875. doi: 
      10.1016/j.bbrc.2018.02.151. Epub 2018 Feb 20.