PMID- 29471003 OWN - NLM STAT- MEDLINE DCOM- 20190218 LR - 20190219 IS - 1872-7972 (Electronic) IS - 0304-3940 (Linking) VI - 672 DP - 2018 Apr 13 TI - TrkB receptor antagonism inhibits stab injury induced proliferative response in adult zebrafish (Danio rerio) brain. PG - 28-33 LID - S0304-3940(18)30122-8 [pii] LID - 10.1016/j.neulet.2018.02.040 [doi] AB - The Tropomyosin related kinase B (TrkB) receptor, is known to promote neuronal maturation, differentiation, maintenance and survival through its cognate ligands Brain derived neurotrophic factor (BDNF) and neurotrophin 4 (NT4). BDNF, NT4 and TrkB are highly conserved proteins among vertebrates. Although the role of TrkB during brain development is well established, its role in adult neurogenesis and brain regeneration awaits thorough investigation. In this study, we used the zebrafish stab wound injury model to determine whether the injury induced regeneration response in the telencephalon region is governed by TrkB or not. We induced stab wound injury in the mid-dorsal region of telencephalon of ANA-12 (selective TrkB antagonist) treated and non-treated zebrafish brain and examined the proliferation activity in selected brain regions using immunohistochemistry. We found that proliferation activity was significantly low in ANA-12 injected injured fishes as compared to vehicle control injured fishes. Other major findings of the study include the temporal pattern of proliferation activity after an injury and activation of adult neural stem cells (aNSCs) situated distantly apart from the injury site in the adult zebrafish brain. CI - Copyright (c) 2018 Elsevier B.V. All rights reserved. FAU - Anand, Surendra Kumar AU - Anand SK AD - Laboratory of Cellular & Molecular Neurobiology, School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067, India. FAU - Mondal, Amal Chandra AU - Mondal AC AD - Laboratory of Cellular & Molecular Neurobiology, School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, 110067, India. Electronic address: acmondal@mail.jnu.ac.in. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180220 PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (ANA 12 compound) RN - 0 (Azepines) RN - 0 (Benzamides) RN - 0 (Proliferating Cell Nuclear Antigen) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Animals MH - Azepines/*pharmacology MH - Benzamides/*pharmacology MH - Brain/*drug effects/metabolism MH - Cell Proliferation/*drug effects MH - Proliferating Cell Nuclear Antigen/metabolism MH - Receptor, trkB/*antagonists & inhibitors/metabolism MH - Wound Healing/*drug effects MH - Zebrafish OTO - NOTNLM OT - Adult zebrafish OT - BDNF OT - Brain regeneration OT - Stab injury OT - TrkB EDAT- 2018/02/23 06:00 MHDA- 2019/02/20 06:00 CRDT- 2018/02/23 06:00 PHST- 2017/11/22 00:00 [received] PHST- 2018/02/09 00:00 [revised] PHST- 2018/02/18 00:00 [accepted] PHST- 2018/02/23 06:00 [pubmed] PHST- 2019/02/20 06:00 [medline] PHST- 2018/02/23 06:00 [entrez] AID - S0304-3940(18)30122-8 [pii] AID - 10.1016/j.neulet.2018.02.040 [doi] PST - ppublish SO - Neurosci Lett. 2018 Apr 13;672:28-33. doi: 10.1016/j.neulet.2018.02.040. Epub 2018 Feb 20.