PMID- 29471139
OWN - NLM
STAT- MEDLINE
DCOM- 20180828
LR  - 20181202
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 117
DP  - 2018 Apr
TI  - TLR2/MyD88/NF-kappaB signaling pathway regulates IL-1beta production in DF-1 cells 
      exposed to Mycoplasma gallisepticum LAMPs.
PG  - 225-231
LID - S0882-4010(17)31693-5 [pii]
LID - 10.1016/j.micpath.2018.02.037 [doi]
AB  - Mycoplasma gallisepticum (M. gallisepticum) is one of the most important 
      pathogens that cause chronic respiratory disease in chickens. M. 
      gallisepticum-derived lipid-associated membrane proteins (LAMPs) are thought to 
      be one of the major factors in mycoplasma pathogenesis and are potent inducers of 
      the host innate immune response. However, the interaction of pathogenic M. 
      gallisepticum-derived LAMPs with Toll-like receptors (TLRs) and the signaling 
      pathways responsible for activating inflammation and NF-kappaB have not been fully 
      elucidated. In this study, we found that IL-1beta expression was induced in 
      DF-1 cells stimulated with M. gallisepticum LAMPs. Subcellular localization 
      experiments using immunofluorescence assays (IFAs) showed p65 translocation from 
      the cytoplasm to the nucleus in DF-1 cells following stimulation with M. 
      gallisepticum LAMPs. Phosphorylation of p65 was detected in LAMP-stimulated 
      DF-1 cells. Treatment with an NF-kappaB-specific inhibitor showed that NF-kappaB is 
      required for M. gallisepticum LAMP-induced IL-1beta expression. In addition, the 
      results indicated that TLR2 and myeloid differentiation primary-response protein 
      88 (MyD88)-dependent signaling pathways were involved in the activation of NF-kappaB 
      by M. gallisepticum LAMPs. Together, these results provide evidence that M. 
      gallisepticum LAMPs activate IL-1beta production through the NF-kappaB pathway via TLR2 
      and MyD88.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Yu, Ying
AU  - Yu Y
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, PR China.
FAU - Chen, Ying
AU  - Chen Y
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, PR China; 
      College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Wang, Yang
AU  - Wang Y
AD  - Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei 
      Provincial Cooperative Innovation Center of Industrial Fermentation, College of 
      Bioengineering, Hubei University of Technology, Wuhan, China.
FAU - Li, Yuan
AU  - Li Y
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, PR China.
FAU - Zhang, Lin
AU  - Zhang L
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, PR China.
FAU - Xin, Jiuqing
AU  - Xin J
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, PR China. 
      Electronic address: xinjiuqing@caas.cn.
LA  - eng
PT  - Journal Article
DEP - 20180219
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Toll-Like Receptor 2)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/immunology/metabolism
MH  - Bacterial Proteins/pharmacology
MH  - Cell Line
MH  - Chickens
MH  - Gene Expression Regulation/drug effects
MH  - Host-Pathogen Interactions/immunology
MH  - Immunity, Innate
MH  - Inflammation/immunology
MH  - Interleukin-1beta/*biosynthesis/genetics/metabolism
MH  - Mycoplasma Infections/*immunology
MH  - Mycoplasma gallisepticum/immunology/*metabolism/pathogenicity
MH  - Myeloid Differentiation Factor 88/*biosynthesis/genetics
MH  - *Signal Transduction
MH  - Toll-Like Receptor 2/*biosynthesis/genetics
OTO - NOTNLM
OT  - IL-1beta
OT  - M. gallisepticum-Derived LAMPs
OT  - NF-kappaB
EDAT- 2018/02/23 06:00
MHDA- 2018/08/29 06:00
CRDT- 2018/02/23 06:00
PHST- 2017/12/09 00:00 [received]
PHST- 2018/02/10 00:00 [revised]
PHST- 2018/02/17 00:00 [accepted]
PHST- 2018/02/23 06:00 [pubmed]
PHST- 2018/08/29 06:00 [medline]
PHST- 2018/02/23 06:00 [entrez]
AID - S0882-4010(17)31693-5 [pii]
AID - 10.1016/j.micpath.2018.02.037 [doi]
PST - ppublish
SO  - Microb Pathog. 2018 Apr;117:225-231. doi: 10.1016/j.micpath.2018.02.037. Epub 
      2018 Feb 19.