PMID- 29472137
OWN - NLM
STAT- MEDLINE
DCOM- 20181018
LR  - 20181018
IS  - 2300-732X (Electronic)
IS  - 1642-431X (Linking)
VI  - 18
IP  - 2
DP  - 2018 Jun
TI  - Synthesis of steroid hormones in the porcine oviduct during early pregnancy.
PG  - 143-150
LID - S1642-431X(17)30266-8 [pii]
LID - 10.1016/j.repbio.2018.02.002 [doi]
AB  - Past studies of the oviducts have documented oviductal steroid production during 
      the oestrous cycle in pigs. The present study examined whether the pig oviducts 
      are the source of steroid hormones during early pregnancy. In the ampulla and 
      isthmus, the expression of 3beta-hydroxysteroid dehydrogenase (3betaHSD) and aromatase 
      cytochrome P450 (CYP19) mRNA by real-time PCR, cellular localization and 
      quantities of the studied proteins by immunofluorescence and Western blot 
      analysis, and concentration of steroid hormones in oviductal flushings by 
      radioimmunoassay, were studied. The expression of 3betaHSD in the ampulla and 
      isthmus was correlated (r = 0.89) and higher on Days 2-3 and 15-16 than on Days 
      10-11 and 12-13. CYP19 expression was elevated in the ampulla on Days 2-3, 10-11 
      and 15-16 and in the isthmus on Days 2-3 vs. the other days studied. The studied 
      proteins were localized in oviductal epithelial cells. In the ampulla, the 
      quantity of 3betaHSD protein did not change, and was greater in the isthmus on Days 
      2-3 vs. Days 12-13 of pregnancy. The P450arom protein quantity increased in the 
      ampulla on Days 2-3 vs. Days 10-11 and 15-16 and vs. Days 10-11 and 12-13 in the 
      isthmus. The concentrations of progesterone and androstenedione in oviductal 
      flushings were lowest on Days 12-13 and on Days 2-3 and 15-16, respectively, 
      while oestradiol-17beta and oestrone levels did not change. Porcine oviducts are the 
      sources of steroid hormones during early pregnancy. The expression of 
      steroidogenic enzymes primarily increases during the embryos presence in the 
      oviduct, i.e., on Days 2-3 of pregnancy.
CI  - Copyright (c) 2018 Society for Biology of Reproduction & the Institute of Animal 
      Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. 
      Published by Elsevier B.V. All rights reserved.
FAU - Martyniak, Marcin
AU  - Martyniak M
AD  - University of Warmia and Mazury in Olsztyn, Department of Animal Physiology 
      Oczapowskiego 1A, 10-719, Olsztyn, Poland. Electronic address: 
      marcin.martyniak@uwm.edu.pl.
FAU - Franczak, Anita
AU  - Franczak A
AD  - University of Warmia and Mazury in Olsztyn, Department of Animal Physiology 
      Oczapowskiego 1A, 10-719, Olsztyn, Poland.
FAU - Kotwica, Genowefa
AU  - Kotwica G
AD  - University of Warmia and Mazury in Olsztyn, Department of Animal Physiology 
      Oczapowskiego 1A, 10-719, Olsztyn, Poland.
LA  - eng
PT  - Journal Article
DEP - 20180219
PL  - Poland
TA  - Reprod Biol
JT  - Reproductive biology
JID - 101160559
RN  - 2DI9HA706A (Estrone)
RN  - 409J2J96VR (Androstenedione)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.51 (3 (or 17)-beta-hydroxysteroid dehydrogenase)
RN  - EC 1.14.14.1 (Aromatase)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
SB  - IM
MH  - 17-Hydroxysteroid Dehydrogenases/metabolism
MH  - Androstenedione/*biosynthesis
MH  - Animals
MH  - Aromatase/metabolism
MH  - Estradiol/*biosynthesis
MH  - Estrone/*biosynthesis
MH  - Female
MH  - Oviducts/*metabolism
MH  - Pregnancy
MH  - *Pregnancy, Animal
MH  - Progesterone/*biosynthesis
MH  - Steroid 17-alpha-Hydroxylase/metabolism
MH  - Swine
OTO - NOTNLM
OT  - 3beta-hydroxysteroid dehydrogenase
OT  - Early pregnancy
OT  - Oviductal environment
OT  - P450 aromatase
OT  - Pig
EDAT- 2018/02/24 06:00
MHDA- 2018/10/20 06:00
CRDT- 2018/02/24 06:00
PHST- 2017/10/02 00:00 [received]
PHST- 2018/02/04 00:00 [revised]
PHST- 2018/02/08 00:00 [accepted]
PHST- 2018/02/24 06:00 [pubmed]
PHST- 2018/10/20 06:00 [medline]
PHST- 2018/02/24 06:00 [entrez]
AID - S1642-431X(17)30266-8 [pii]
AID - 10.1016/j.repbio.2018.02.002 [doi]
PST - ppublish
SO  - Reprod Biol. 2018 Jun;18(2):143-150. doi: 10.1016/j.repbio.2018.02.002. Epub 2018 
      Feb 19.