PMID- 29472541
OWN - NLM
STAT- MEDLINE
DCOM- 20180501
LR  - 20190222
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 9
IP  - 1
DP  - 2018 Feb 22
TI  - Periosteum contains skeletal stem cells with high bone regenerative potential 
      controlled by Periostin.
PG  - 773
LID - 10.1038/s41467-018-03124-z [doi]
LID - 773
AB  - Bone regeneration relies on the activation of skeletal stem cells (SSCs) that 
      still remain poorly characterized. Here, we show that periosteum contains SSCs 
      with high bone regenerative potential compared to bone marrow stromal 
      cells/skeletal stem cells (BMSCs) in mice. Although periosteal cells (PCs) and 
      BMSCs are derived from a common embryonic mesenchymal lineage, postnatally PCs 
      exhibit greater clonogenicity, growth and differentiation capacity than BMSCs. 
      During bone repair, PCs can efficiently contribute to cartilage and bone, and 
      integrate long-term after transplantation. Molecular profiling uncovers genes 
      encoding Periostin and other extracellular matrix molecules associated with the 
      enhanced response to injury of PCs. Periostin gene deletion impairs PC functions 
      and fracture consolidation. Periostin-deficient periosteum cannot reconstitute a 
      pool of PCs after injury demonstrating the presence of SSCs within periosteum and 
      the requirement of Periostin in maintaining this pool. Overall our results 
      highlight the importance of analyzing periosteum and PCs to understand bone 
      phenotypes.
FAU - Duchamp de Lageneste, Oriane
AU  - Duchamp de Lageneste O
AD  - INSERM UMR1163, Imagine Institute, Paris Descartes University, 75015, Paris, 
      France.
FAU - Julien, Anais
AU  - Julien A
AD  - INSERM UMR1163, Imagine Institute, Paris Descartes University, 75015, Paris, 
      France.
FAU - Abou-Khalil, Rana
AU  - Abou-Khalil R
AD  - INSERM UMR1163, Imagine Institute, Paris Descartes University, 75015, Paris, 
      France.
FAU - Frangi, Giulia
AU  - Frangi G
AD  - INSERM UMR1163, Imagine Institute, Paris Descartes University, 75015, Paris, 
      France.
FAU - Carvalho, Caroline
AU  - Carvalho C
AD  - INSERM UMR1163, Imagine Institute, Paris Descartes University, 75015, Paris, 
      France.
FAU - Cagnard, Nicolas
AU  - Cagnard N
AD  - Paris-Descartes Bioinformatics Platform, 75015, Paris, France.
FAU - Cordier, Corinne
AU  - Cordier C
AD  - INSERM US24 - CNRS UMS3633 Cytometry Platform, Paris Descartes University, 75015, 
      Paris, France.
FAU - Conway, Simon J
AU  - Conway SJ
AD  - Herman B. Wells Center for Pediatric Research, Department of Pediatrics, Indiana 
      University School of Medicine, Indianapolis, IN, 46202, USA.
FAU - Colnot, Celine
AU  - Colnot C
AUID- ORCID: 0000-0001-8423-8718
AD  - INSERM UMR1163, Imagine Institute, Paris Descartes University, 75015, Paris, 
      France. celine.colnot@inserm.fr.
LA  - eng
GR  - R01 AR057344/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180222
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Postn protein, mouse)
SB  - IM
MH  - Animals
MH  - *Bone Regeneration
MH  - Cell Adhesion Molecules/genetics/*metabolism
MH  - Female
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Osteogenesis
MH  - Periosteum/*cytology/metabolism
MH  - Stem Cells/cytology/*metabolism
MH  - Stromal Cells/cytology/metabolism
PMC - PMC5823889
COIS- The authors declare no competing financial interests.
EDAT- 2018/02/24 06:00
MHDA- 2018/05/02 06:00
PMCR- 2018/02/22
CRDT- 2018/02/24 06:00
PHST- 2017/02/09 00:00 [received]
PHST- 2018/01/19 00:00 [accepted]
PHST- 2018/02/24 06:00 [entrez]
PHST- 2018/02/24 06:00 [pubmed]
PHST- 2018/05/02 06:00 [medline]
PHST- 2018/02/22 00:00 [pmc-release]
AID - 10.1038/s41467-018-03124-z [pii]
AID - 3124 [pii]
AID - 10.1038/s41467-018-03124-z [doi]
PST - epublish
SO  - Nat Commun. 2018 Feb 22;9(1):773. doi: 10.1038/s41467-018-03124-z.