PMID- 29475460
OWN - NLM
STAT- MEDLINE
DCOM- 20180830
LR  - 20220330
IS  - 2410-8650 (Electronic)
IS  - 1607-551X (Linking)
VI  - 34
IP  - 3
DP  - 2018 Mar
TI  - Brain-derived neurotrophic factor preserves intestinal mucosal barrier function 
      and alters gut microbiota in mice.
PG  - 134-141
LID - S1607-551X(17)30317-0 [pii]
LID - 10.1016/j.kjms.2017.11.002 [doi]
AB  - The intestinal mucosal barrier (IMB) enables the intestine to provide adequate 
      containment of luminal microorganisms and molecules while preserving the ability 
      to absorb nutrients. In this study, we explored the effect of brain-derived 
      neurotrophic factor (BDNF) on IMB function and gut microbiota in mice. BDNF gene 
      knock-out mice (the BDNF(+/-) group) and wild-type mice (the BDNF(+/+) group) 
      were selected. The gut microbiota of these mice was analyzed by denaturing 
      gradient gel electrophoresis (DGGE) assay. The ultrastructure of the ileum and 
      the colonic epithelium obtained from decapitated mice were observed by 
      transmission electron microscopy. The protein expression of epithelial tight 
      junction proteins, zonula occludens-1 (ZO-1) and occludin was detected by 
      immunohistochemistry staining. The protein expression of claudin-1 and claudin-2 
      was determined by Western blotting. The DGGE band patterns of gut microbiota in 
      the BDNF(+/-) group were significantly different from that in the BDNF(+/+) 
      group, which indicated that the BDNF expression alters the gut microbiota in 
      mice. Compared with the BDNF(+/+) group, the BDNF(+/-) group presented no 
      significant difference in the ultrastructure of ileal epithelium; however, a 
      significant difference was observed in the colonic epithelial barrier, manifested 
      by decreased microvilli, widening intercellular space and bacterial invasion. 
      Compared with the BDNF(+/+) group, the expression of ZO-1 and occludin in the 
      BDNF(+/-) group was significantly decreased. The expression of claudin-1 in the 
      BDNF(+/-) group was significantly reduced, while the expression of claudin-2 was 
      elevated. These findings indicate that BDNF preserves IMB function and modulates 
      gut microbiota in mice.
CI  - Copyright (c) 2018. Published by Elsevier Taiwan.
FAU - Li, Chen
AU  - Li C
AD  - The Fifth Department of Pediatrics Medicine, Cangzhou Central Hospital, Cangzhou, 
      PR China.
FAU - Cai, Yong-Yan
AU  - Cai YY
AD  - The First Department of Pediatrics Medicine, Cangzhou Central Hospital, Cangzhou, 
      PR China. Electronic address: caiyy0816@163.com.
FAU - Yan, Zhi-Xin
AU  - Yan ZX
AD  - The First Department of Pediatrics Medicine, Cangzhou Central Hospital, Cangzhou, 
      PR China.
LA  - eng
PT  - Journal Article
PL  - China (Republic : 1949- )
TA  - Kaohsiung J Med Sci
JT  - The Kaohsiung journal of medical sciences
JID - 100960562
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Claudin-1)
RN  - 0 (Claudin-2)
RN  - 0 (Occludin)
RN  - 0 (Ocln protein, mouse)
RN  - 0 (Tjp1 protein, mouse)
RN  - 0 (Zonula Occludens-1 Protein)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/deficiency/*genetics
MH  - Claudin-1/genetics/metabolism
MH  - Claudin-2/genetics/metabolism
MH  - Colon/metabolism/*microbiology/ultrastructure
MH  - Gastrointestinal Microbiome/*physiology
MH  - Gene Expression Regulation
MH  - Ileum/metabolism/*microbiology/ultrastructure
MH  - Intestinal Mucosa/metabolism/*microbiology/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Microvilli/metabolism/microbiology/ultrastructure
MH  - Occludin/genetics/metabolism
MH  - Tight Junctions/metabolism/microbiology/ultrastructure
MH  - Zonula Occludens-1 Protein/genetics/metabolism
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - Gut microbiota
OT  - Intestinal mucosal barrier function
OT  - Occludin
OT  - ZO-1
EDAT- 2018/02/25 06:00
MHDA- 2018/08/31 06:00
CRDT- 2018/02/25 06:00
PHST- 2017/05/10 00:00 [received]
PHST- 2017/08/07 00:00 [revised]
PHST- 2017/11/03 00:00 [accepted]
PHST- 2018/02/25 06:00 [entrez]
PHST- 2018/02/25 06:00 [pubmed]
PHST- 2018/08/31 06:00 [medline]
AID - S1607-551X(17)30317-0 [pii]
AID - 10.1016/j.kjms.2017.11.002 [doi]
PST - ppublish
SO  - Kaohsiung J Med Sci. 2018 Mar;34(3):134-141. doi: 10.1016/j.kjms.2017.11.002.