PMID- 29476339
OWN - NLM
STAT- MEDLINE
DCOM- 20180710
LR  - 20181202
IS  - 1432-1955 (Electronic)
IS  - 0932-0113 (Linking)
VI  - 117
IP  - 4
DP  - 2018 Apr
TI  - Role of glutathione, ROS, and Bcl-xL in the inhibition of apoptosis of 
      monocyte-derived dendritic cells by Leishmania mexicana promastigotes.
PG  - 1225-1235
LID - 10.1007/s00436-018-5804-z [doi]
AB  - Dendritic cells (DCs) are one of the principal host cells of the obligate 
      intracellular parasite Leishmania that can survive and reproduce within cells due 
      to the ability to regulate different cellular events, including apoptosis. 
      Inhibition of host cell apoptosis is a strategy employed by multiple pathogens to 
      ensure their survival in the infected cell. We have previously reported that 
      Leishmania mexicana promastigotes and amastigotes inhibit camptothecin-induced 
      apoptosis of monocyte-derived dendritic cells (moDCs) through the downregulation 
      of p38 and JNK phosphorylation. The upregulation of glutathione (GSH), the most 
      important regulator of reactive oxygen species (ROS) concentration, has proven to 
      protect cells from apoptosis through the inhibition of JNK1. Another mechanism 
      employed by cells for the protection of apoptosis is the expression of 
      anti-apoptotic proteins of the Bcl-2 family. The aim of this study was to 
      determine if GSH, ROS, and Bcl-xL participate in the inhibition of 
      camptothecin-induced apoptosis of moDC by L. mexicana promastigotes. GSH 
      quantification assays showed that camptothecin and BSO (an inhibitor of 
      glutathione synthesis) strongly decreased intracellular GSH concentration in 
      moDC, while infection with L. mexicana promastigotes had no effect in the level 
      of GSH. On the other hand, infection with L. mexicana promastigotes of BSO- and 
      camptothecin-treated moDC diminished the concentration of ROS and induced the 
      expression of the anti-apoptotic protein Bcl-xL. Our findings suggest that 
      inhibition of camptothecin-induced apoptosis of moDC by L. mexicana promastigotes 
      is preferentially regulated by the expression of anti-apoptotic proteins of the 
      Bcl-2 family rather than by the redox status of the cell.
FAU - Rodriguez-Gonzalez, Jorge
AU  - Rodriguez-Gonzalez J
AD  - Unidad Periferica de Investigacion UNAM-INC, Division de Investigacion, Facultad 
      de Medicina, Universidad Nacional Autonoma de Mexico-Instituto Nacional de 
      Cardiologia "Ignacio Chavez", Juan Badiano No. 1, Col. Belisario Dominguez, 
      Seccion XVI, Delegacion Tlalpan, C.P, 14080, Ciudad de Mexico, Mexico.
AD  - Posgrado en Ciencias Biologicas, Facultad de Medicina, Unidad de Posgrado, Ciudad 
      Universitaria, 04510, Ciudad de Mexico, Mexico.
FAU - Wilkins-Rodriguez, Arturo A
AU  - Wilkins-Rodriguez AA
AD  - Unidad Periferica de Investigacion UNAM-INC, Division de Investigacion, Facultad 
      de Medicina, Universidad Nacional Autonoma de Mexico-Instituto Nacional de 
      Cardiologia "Ignacio Chavez", Juan Badiano No. 1, Col. Belisario Dominguez, 
      Seccion XVI, Delegacion Tlalpan, C.P, 14080, Ciudad de Mexico, Mexico.
FAU - Gutierrez-Kobeh, Laila
AU  - Gutierrez-Kobeh L
AD  - Unidad Periferica de Investigacion UNAM-INC, Division de Investigacion, Facultad 
      de Medicina, Universidad Nacional Autonoma de Mexico-Instituto Nacional de 
      Cardiologia "Ignacio Chavez", Juan Badiano No. 1, Col. Belisario Dominguez, 
      Seccion XVI, Delegacion Tlalpan, C.P, 14080, Ciudad de Mexico, Mexico. 
      lgutierr@unam.mx.
LA  - eng
GR  - IN 225116/Papiit, DGAPA, UNAM/
PT  - Journal Article
DEP - 20180223
PL  - Germany
TA  - Parasitol Res
JT  - Parasitology research
JID - 8703571
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (bcl-X Protein)
RN  - 5072-26-4 (Buthionine Sulfoximine)
RN  - GAN16C9B8O (Glutathione)
RN  - XT3Z54Z28A (Camptothecin)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Buthionine Sulfoximine/pharmacology
MH  - Camptothecin/pharmacology
MH  - Cells, Cultured
MH  - Dendritic Cells/*parasitology/*physiology
MH  - Down-Regulation
MH  - Glutathione/*metabolism
MH  - Humans
MH  - Leishmania mexicana/*immunology
MH  - Phosphorylation
MH  - Reactive Oxygen Species/*metabolism
MH  - bcl-X Protein/*metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Bcl-xL
OT  - Dendritic cells
OT  - Glutathione
OT  - Leishmania mexicana
EDAT- 2018/02/25 06:00
MHDA- 2018/07/11 06:00
CRDT- 2018/02/25 06:00
PHST- 2017/09/20 00:00 [received]
PHST- 2018/02/08 00:00 [accepted]
PHST- 2018/02/25 06:00 [pubmed]
PHST- 2018/07/11 06:00 [medline]
PHST- 2018/02/25 06:00 [entrez]
AID - 10.1007/s00436-018-5804-z [pii]
AID - 10.1007/s00436-018-5804-z [doi]
PST - ppublish
SO  - Parasitol Res. 2018 Apr;117(4):1225-1235. doi: 10.1007/s00436-018-5804-z. Epub 
      2018 Feb 23.