PMID- 29476476
OWN - NLM
STAT- MEDLINE
DCOM- 20190114
LR  - 20190114
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1745
DP  - 2018
TI  - The Retinal Pigment Epithelial Cell Line (ARPE-19) Displays Mosaic Structural 
      Chromosomal Aberrations.
PG  - 305-314
LID - 10.1007/978-1-4939-7680-5_17 [doi]
AB  - The retinal pigment epithelial cell line ARPE-19 was established in 1996 and 
      remains widely used today for biomedical and in particular ophthalmology 
      research. We have analyzed the chromosomes of the ARPE-19 cell line and found 
      cultured cells exist as a heterogeneous mixture having both normal karyotypes and 
      chromosomal rearrangements. In ARPE-19 cells, we observed metaphases with a 
      single translocation t(15;19) and metaphases with two translocations t(5;15) and 
      t(15;19) and a derivative chromosome 9. Aneuploidies have also been detected 
      (monosomy: -16; trisomy: +11, +18). Multiple attempts to isolate clones with a 
      normal karyotype from those with aberrant karyotypes failed due to senescence of 
      cells of normal karyotypes. We could, however, isolate clones with the 
      translocation t(15;19) and clones with two translocations t(5;15) and t(15;19). 
      In continued cell culture after second subcloning for 30 passages, all clones 
      maintained their cytogenetic integrity.We have further investigated the 
      chromosomal profiles of the ARPE-19 cell line from another laboratory and 
      observed cells with a normal karyotype as well as abnormalities in chromosomes 6p 
      and 11q. The DNA profiles of the ARPE-19 cells from both labs were identical to 
      the ATCC profiles, excluding contamination with other cell lines. Since 
      chromosomal translocations in ARPE-19 cells differ from lab to lab and display a 
      mosaicism for structural chromosomal aberrations, researchers dealing with 
      ARPE-19 cells should screen their stocks for chromosomal aberrations and proceed 
      with caution against misinterpretations during experimental manipulations with 
      this cell line. This chapter describes in detail our laboratory methods for 
      single cell cloning, karyotype analysis and fluorescence in situ hybridization 
      (FISH), which we used for the identification and characterization of chromosomal 
      translocations in the retinal pigment epithelial cell line ARPE-19.
FAU - Fasler-Kan, Elizaveta
AU  - Fasler-Kan E
AD  - Department of Biomedicine, University of Basel and University Hospital Basel, 
      Basel, Switzerland. elizaveta.fasler@insel.ch.
AD  - Department of Pediatric Surgery, Inselspital, University Hospital Bern and 
      Department of Biomedical Research, University of Bern, Bern, Switzerland. 
      elizaveta.fasler@insel.ch.
FAU - Aliu, Nijas
AU  - Aliu N
AD  - Department of Human Genetics, University Children's Hospital, Inselspital, Bern, 
      Switzerland.
FAU - Wunderlich, Kerstin
AU  - Wunderlich K
AD  - Department of Biomedicine, University of Basel and University Hospital Basel, 
      Basel, Switzerland.
AD  - Department of Ophthalmology, University of Basel, Basel, Switzerland.
FAU - Ketterer, Sylvia
AU  - Ketterer S
AD  - Department of Biomedicine, University of Basel and University Hospital Basel, 
      Basel, Switzerland.
FAU - Ruggiero, Sabrina
AU  - Ruggiero S
AD  - Department of Pediatric Surgery, Inselspital, University Hospital Bern and 
      Department of Biomedical Research, University of Bern, Bern, Switzerland.
FAU - Berger, Steffen
AU  - Berger S
AD  - Department of Pediatric Surgery, Inselspital, University Hospital Bern and 
      Department of Biomedical Research, University of Bern, Bern, Switzerland.
FAU - Meyer, Peter
AU  - Meyer P
AD  - Department of Ophthalmology, University of Basel, Basel, Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Cell Line
MH  - *Chromosome Aberrations
MH  - Epithelial Cells/*metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - *Mosaicism
MH  - Retinal Pigment Epithelium/*cytology
MH  - Single-Cell Analysis
MH  - Translocation, Genetic
OTO - NOTNLM
OT  - ARPE-19 cell line
OT  - Chromosomal aberrations
OT  - Chromosomal translocations
OT  - FISH
OT  - Heterogeneity
OT  - Karyotype
OT  - Mosaicism
OT  - Retinal pigment cells
OT  - Single-cell cloning
EDAT- 2018/02/25 06:00
MHDA- 2019/01/15 06:00
CRDT- 2018/02/25 06:00
PHST- 2018/02/25 06:00 [entrez]
PHST- 2018/02/25 06:00 [pubmed]
PHST- 2019/01/15 06:00 [medline]
AID - 10.1007/978-1-4939-7680-5_17 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1745:305-314. doi: 10.1007/978-1-4939-7680-5_17.