PMID- 29477540
OWN - NLM
STAT- MEDLINE
DCOM- 20180920
LR  - 20191210
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 113
DP  - 2018 Jul 1
TI  - In vitro DNA binding studies of therapeutic and prophylactic drug citral.
PG  - 300-308
LID - S0141-8130(17)34952-8 [pii]
LID - 10.1016/j.ijbiomac.2018.02.098 [doi]
AB  - The study of drug-DNA interactions is of great importance, as it paves the way 
      towards the design of better therapeutic agents. Here, the interaction of DNA 
      with a therapeutic and prophylactic drug citral has been studied. We have 
      attempted to ascertain the mode of binding of citral with calf thymus DNA 
      (Ct-DNA) through various biophysical techniques. Analysis of the UV-visible 
      absorbance spectra and fluorescence spectra indicated the formation of a complex 
      between citral and Ct-DNA. Competitive binding assays with ethidium bromide (EB), 
      acridine orange (AO) and Hoechst 33258 reflected that citral possibly 
      intercalates within the Ct-DNA. These observations were further confirmed by 
      circular dichroism (CD) spectral analysis, viscosity measurements, DNA melting 
      and molecular docking studies. This study is expected to contribute to a better 
      understanding of molecular mechanisms of citral, and design of new drugs in the 
      future.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Alam, Md Fazle
AU  - Alam MF
AD  - Enzymology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim 
      University, Aligarh 202002, India.
FAU - Varshney, Supriya
AU  - Varshney S
AD  - Enzymology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim 
      University, Aligarh 202002, India.
FAU - Khan, Masood Alam
AU  - Khan MA
AD  - College of Applied Medical Sciences, Qassim University, Buraidah, Saudi Arabia.
FAU - Laskar, Amaj Ahmed
AU  - Laskar AA
AD  - Enzymology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim 
      University, Aligarh 202002, India.
FAU - Younus, Hina
AU  - Younus H
AD  - Enzymology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim 
      University, Aligarh 202002, India. Electronic address: hyounus.cb@amu.ac.in.
LA  - eng
PT  - Journal Article
DEP - 20180222
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Acyclic Monoterpenes)
RN  - 0 (Monoterpenes)
RN  - 9007-49-2 (DNA)
RN  - 91080-16-9 (calf thymus DNA)
RN  - T7EU0O9VPP (citral)
SB  - IM
MH  - Acyclic Monoterpenes
MH  - Animals
MH  - Cattle
MH  - DNA/*chemistry/*metabolism
MH  - Molecular Docking Simulation
MH  - Monoterpenes/*metabolism
MH  - Nucleic Acid Denaturation
MH  - Temperature
MH  - Viscosity
OTO - NOTNLM
OT  - Binding
OT  - Citral
OT  - DNA
EDAT- 2018/02/27 06:00
MHDA- 2018/09/21 06:00
CRDT- 2018/02/26 06:00
PHST- 2017/12/12 00:00 [received]
PHST- 2018/02/10 00:00 [revised]
PHST- 2018/02/14 00:00 [accepted]
PHST- 2018/02/27 06:00 [pubmed]
PHST- 2018/09/21 06:00 [medline]
PHST- 2018/02/26 06:00 [entrez]
AID - S0141-8130(17)34952-8 [pii]
AID - 10.1016/j.ijbiomac.2018.02.098 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2018 Jul 1;113:300-308. doi: 10.1016/j.ijbiomac.2018.02.098. 
      Epub 2018 Feb 22.