PMID- 2948111
OWN - NLM
STAT- MEDLINE
DCOM- 19870127
LR  - 20190824
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 23
IP  - 7
DP  - 1986 Jul
TI  - Cross-linking of IgE-receptor complexes at the cell surface: synthesis and 
      characterization of a long bivalent hapten that is capable of triggering mast 
      cells and rat basophilic leukemia cells.
PG  - 783-90
AB  - The ability of a series of bivalent haptens to bind and cross-link immunoglobulin 
      E (IgE) in solution and on the surface of cells was examined. Several short (less 
      than 30 A) dinitrophenyl (DNP) haptens were found to bind tightly to and 
      cross-link a monoclonal anti-DNP IgE in solution, but these failed to trigger 
      substantial release of 3H-serotonin from sensitized rat basophilic leukemia (RBL) 
      cells or rat peritoneal mast cells. A longer bivalent hapten, approximately 50 A 
      in length, consisting of two DNP-aminocaproyl-L-tyrosine (DCT) groups coupled to 
      the alpha-amino groups of L-cystine was synthesized and characterized. This 
      bivalent hapten [(DCT)2-cystine], binds very tightly to the same monoclonal 
      anti-DNP IgE in solution and cross-links these antibodies to form higher mol. wt 
      aggregates as judged by gel filtration and binding studies. It also stimulates 
      degranulation of both RBL and mast cells sensitized with two different monoclonal 
      anti-DNP IgE antibodies, with the mast cells exhibiting generally greater 
      responsiveness to this ligand. The (DCT)2-cystine bivalent hapten appears to have 
      the structural features necessary for carrying out detailed binding studies with 
      receptor-bound IgE on the cell surface.
FAU - Kane, P
AU  - Kane P
FAU - Erickson, J
AU  - Erickson J
FAU - Fewtrell, C
AU  - Fewtrell C
FAU - Baird, B
AU  - Baird B
FAU - Holowka, D
AU  - Holowka D
LA  - eng
GR  - AI 18306/AI/NIAID NIH HHS/United States
GR  - AI 18610/AI/NIAID NIH HHS/United States
GR  - GM 13292/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Haptens)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
RN  - 333DO1RDJY (Serotonin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Dinitrobenzenes/immunology
MH  - Haptens/*immunology
MH  - Immunoglobulin E/*metabolism
MH  - Leukemia, Experimental/immunology
MH  - Mast Cells/immunology
MH  - Rats
MH  - Receptors, Fc/*metabolism
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*metabolism
MH  - Serotonin/metabolism
EDAT- 1986/07/01 00:00
MHDA- 1986/07/01 00:01
CRDT- 1986/07/01 00:00
PHST- 1986/07/01 00:00 [pubmed]
PHST- 1986/07/01 00:01 [medline]
PHST- 1986/07/01 00:00 [entrez]
AID - 10.1016/0161-5890(86)90090-8 [doi]
PST - ppublish
SO  - Mol Immunol. 1986 Jul;23(7):783-90. doi: 10.1016/0161-5890(86)90090-8.