PMID- 29481290
OWN - NLM
STAT- MEDLINE
DCOM- 20190902
LR  - 20240207
IS  - 1535-4970 (Electronic)
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 198
IP  - 1
DP  - 2018 Jul 1
TI  - Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome.
PG  - 67-76
LID - 10.1164/rccm.201710-2033OC [doi]
AB  - RATIONALE: E-cigarettes vaporize propylene glycol/vegetable glycerin (PG/VG), 
      nicotine, and flavorings. However, the long-term health effects of exposing lungs 
      to vaped e-liquids are unknown. OBJECTIVES: To determine the effects of chronic 
      vaping on pulmonary epithelia. METHODS: We performed research bronchoscopies on 
      healthy nonsmokers, cigarette smokers, and e-cigarette users (vapers) and 
      obtained bronchial brush biopsies and lavage samples from these subjects for 
      proteomic investigation. We further employed in vitro and murine exposure models 
      to support our human findings. MEASUREMENTS AND MAIN RESULTS: Visual inspection 
      by bronchoscopy revealed that vaper airways appeared friable and erythematous. 
      Epithelial cells from biopsy samples revealed approximately 300 proteins that 
      were differentially expressed in smoker and vaper airways, with only 78 proteins 
      being commonly altered in both groups and 113 uniquely altered in vapers. For 
      example, CYP1B1 (cytochrome P450 family 1 subfamily B member 1), MUC5AC (mucin 5 
      AC), and MUC4 levels were increased in vapers. Aerosolized PG/VG alone 
      significantly increased MUC5AC protein in human airway epithelial cultures and in 
      murine nasal epithelia in vivo. We also found that e-liquids rapidly entered 
      cells and that PG/VG reduced membrane fluidity and impaired protein diffusion. 
      CONCLUSIONS: We conclude that chronic vaping exerts marked biological effects on 
      the lung and that these effects may in part be mediated by the PG/VG base. These 
      changes are likely not harmless and may have clinical implications for the 
      development of chronic lung disease. Further studies will be required to 
      determine the full extent of vaping on the lung.
FAU - Ghosh, Arunava
AU  - Ghosh A
AD  - 1 Marsico Lung Institute.
FAU - Coakley, Raymond C
AU  - Coakley RC
AD  - 1 Marsico Lung Institute.
FAU - Mascenik, Teresa
AU  - Mascenik T
AD  - 1 Marsico Lung Institute.
FAU - Rowell, Temperance R
AU  - Rowell TR
AD  - 1 Marsico Lung Institute.
AD  - 2 Department of Cell Biology and Physiology.
FAU - Davis, Eric S
AU  - Davis ES
AD  - 1 Marsico Lung Institute.
FAU - Rogers, Keith
AU  - Rogers K
AD  - 1 Marsico Lung Institute.
FAU - Webster, Megan J
AU  - Webster MJ
AD  - 1 Marsico Lung Institute.
FAU - Dang, Hong
AU  - Dang H
AD  - 1 Marsico Lung Institute.
FAU - Herring, Laura E
AU  - Herring LE
AD  - 3 UNC Proteomics Core Facility, Department of Pharmacology.
FAU - Sassano, M Flori
AU  - Sassano MF
AD  - 1 Marsico Lung Institute.
FAU - Livraghi-Butrico, Alessandra
AU  - Livraghi-Butrico A
AD  - 1 Marsico Lung Institute.
FAU - Van Buren, Scott K
AU  - Van Buren SK
AD  - 4 Department of Biostatistics, and.
FAU - Graves, Lee M
AU  - Graves LM
AD  - 3 UNC Proteomics Core Facility, Department of Pharmacology.
FAU - Herman, Melissa A
AU  - Herman MA
AD  - 3 UNC Proteomics Core Facility, Department of Pharmacology.
FAU - Randell, Scott H
AU  - Randell SH
AD  - 1 Marsico Lung Institute.
AD  - 2 Department of Cell Biology and Physiology.
FAU - Alexis, Neil E
AU  - Alexis NE
AD  - 5 Center for Environmental Medicine Asthma and Lung Biology, University of North 
      Carolina at Chapel Hill, North Carolina.
FAU - Tarran, Robert
AU  - Tarran R
AD  - 1 Marsico Lung Institute.
AD  - 2 Department of Cell Biology and Physiology.
LA  - eng
GR  - P30 CA016086/CA/NCI NIH HHS/United States
GR  - P50 CA180890/CA/NCI NIH HHS/United States
GR  - P50 HL120100/HL/NHLBI NIH HHS/United States
GR  - R01 HL135642/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Proteome)
RN  - 6M3C89ZY6R (Nicotine)
SB  - IM
CIN - Am J Respir Crit Care Med. 2018 Jul 1;198(1):6-7. PMID: 29518342
CIN - Am J Respir Crit Care Med. 2018 Nov 15;198(10):1351-1352. PMID: 30153039
CIN - Am J Respir Crit Care Med. 2018 Nov 15;198(10):1350-1351. PMID: 30153045
MH  - Adult
MH  - Bronchi/*drug effects
MH  - *Electronic Nicotine Delivery Systems
MH  - Epithelial Cells/*drug effects
MH  - Female
MH  - Humans
MH  - Lung/*drug effects
MH  - Male
MH  - Middle Aged
MH  - Nicotine/*adverse effects
MH  - Proteome/*drug effects
MH  - *Smokers
PMC - PMC6034122
OTO - NOTNLM
OT  - chronic obstructive pulmonary disease
OT  - mucin
OT  - tobacco
OT  - vaping
EDAT- 2018/02/27 06:00
MHDA- 2019/09/03 06:00
PMCR- 2019/07/01
CRDT- 2018/02/27 06:00
PHST- 2018/02/27 06:00 [pubmed]
PHST- 2019/09/03 06:00 [medline]
PHST- 2018/02/27 06:00 [entrez]
PHST- 2019/07/01 00:00 [pmc-release]
AID - 10.1164/rccm.201710-2033OC [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2018 Jul 1;198(1):67-76. doi: 
      10.1164/rccm.201710-2033OC.