PMID- 29483840
OWN - NLM
STAT- MEDLINE
DCOM- 20190411
LR  - 20190411
IS  - 1449-2288 (Electronic)
IS  - 1449-2288 (Linking)
VI  - 14
IP  - 2
DP  - 2018
TI  - MiR-519d suppresses breast cancer tumorigenesis and metastasis via targeting 
      MMP3.
PG  - 228-236
LID - 10.7150/ijbs.22849 [doi]
AB  - Breast cancer (BC) is the most common cause of death in women throughout the 
      world. Although microRNAs (miRNAs) have been identified as novel regulators in 
      carcinogenesis, there are still abundant hidden treasure needed to be excavated. 
      In the present study, we found that miR-519d expression was remarkably decreased 
      in both human BC tissues and MCF-7 cells. CCK8 and 5-Ethynyl-2'-deoxyuridine 
      (EdU) assays were used to evaluate cell proliferation. Wound-healing and 
      transwell assays were performed for detection of cell migration and invasion. The 
      results demonstrated miR-519d overexpression dramatically suppressed MCF-7 cells 
      proliferation, migration and invasion. While downregulation of miR-519d by 
      miR-519d inhibitor substantially increased MCF-7 cell carcinogenesis. Further 
      analysis identified Matrix Metalloproteinase-3 (MMP3) as a direct target of 
      miR-519d. QRT-PCR and western blot results indicated the correlative expression 
      of miR-519d and MMP3 in BC tissues and MCF-7 cells. In summary, our data 
      uncovered the novel molecular interaction between miR-519d and MMP3, indicating a 
      therapeutic strategy of miR-519d for BC.
FAU - Chu, Chengling
AU  - Chu C
AD  - Department of Oncology, The First Affiliated Hospital of Harbin Medical 
      University, Harbin 150001, China.
FAU - Liu, Xin
AU  - Liu X
AD  - Department of Pharmacology (the State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 
      150081, China.
FAU - Bai, Xue
AU  - Bai X
AD  - Department of Pharmacology (the State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 
      150081, China.
FAU - Zhao, Tong
AU  - Zhao T
AD  - Department of Pharmacology (the State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 
      150081, China.
FAU - Wang, Mengxue
AU  - Wang M
AD  - Department of Pharmacology (the State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 
      150081, China.
FAU - Xu, Ranchen
AU  - Xu R
AD  - Department of Pharmacology (the State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 
      150081, China.
FAU - Li, Mingqi
AU  - Li M
AD  - Department of Pharmacology (the State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 
      150081, China.
FAU - Hu, Yingying
AU  - Hu Y
AD  - Department of Pharmacy, The First Affiliated Hospital of Harbin Medical 
      University, Harbin 150001, China.
FAU - Li, Weihua
AU  - Li W
AD  - Department of Pharmacy, The First Affiliated Hospital of Harbin Medical 
      University, Harbin 150001, China.
FAU - Yang, Lida
AU  - Yang L
AD  - Department of Pharmacology (the State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 
      150081, China.
FAU - Qin, Youyou
AU  - Qin Y
AD  - Department of Breast Surgery, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin 150086, China.
FAU - Yang, Meng
AU  - Yang M
AD  - Department of Oncology, The First Affiliated Hospital of Harbin Medical 
      University, Harbin 150001, China.
FAU - Yan, Chaoqi
AU  - Yan C
AD  - Department of Breast Surgery, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin 150086, China.
FAU - Zhang, Yong
AU  - Zhang Y
AD  - Department of Pharmacology (the State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 
      150081, China.
AD  - Institute of Metabolic Disease, Heilongjiang Academy of Medical Science, Harbin 
      150086, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180209
PL  - Australia
TA  - Int J Biol Sci
JT  - International journal of biological sciences
JID - 101235568
RN  - 0 (MIRN519 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Cell Movement/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - MCF-7 Cells
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - MicroRNAs/genetics/metabolism/*physiology
MH  - Neoplasm Invasiveness/genetics
MH  - Neoplasm Metastasis/genetics
PMC - PMC5821043
OTO - NOTNLM
OT  - MMP3
OT  - breast cancer
OT  - metastasis
OT  - miR-519d
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2018/02/28 06:00
MHDA- 2019/04/12 06:00
PMCR- 2018/01/01
CRDT- 2018/02/28 06:00
PHST- 2017/09/17 00:00 [received]
PHST- 2017/12/28 00:00 [accepted]
PHST- 2018/02/28 06:00 [entrez]
PHST- 2018/02/28 06:00 [pubmed]
PHST- 2019/04/12 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - ijbsv14p0228 [pii]
AID - 10.7150/ijbs.22849 [doi]
PST - epublish
SO  - Int J Biol Sci. 2018 Feb 9;14(2):228-236. doi: 10.7150/ijbs.22849. eCollection 
      2018.