PMID- 29483934
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240313
IS  - 1742-9994 (Print)
IS  - 1742-9994 (Electronic)
IS  - 1742-9994 (Linking)
VI  - 15
DP  - 2018
TI  - Two predominant MUPs, OBP3 and MUP13, are male pheromones in rats.
PG  - 6
LID - 10.1186/s12983-018-0254-0 [doi]
LID - 6
AB  - BACKGROUND: In rats, urine-borne male pheromones comprise organic volatile 
      compounds and major urinary proteins (MUPs). A number of volatile pheromones have 
      been reported, but no MUP pheromones have been identified in rat urine. RESULTS: 
      We used sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), 
      isoelectric focusing electrophoresis (IEF), nano-liquid chromatography-tandem 
      mass spectrometry (nLC-MS/MS) after in gel digestion of the proteins and 
      quantitative real-time PCR (qRT-PCR) and showed that the levels of two MUPs, 
      odorant-binding protein 3 (OBP3) (i.e. PGCL4) and MUP13 (i.e. PGCL1), in urine 
      and their mRNAs in liver were higher in males than in females and were suppressed 
      by orchidectomy and restored by testosterone treatment (T treatment). We then 
      generated recombinant MUPs (rMUPs) and found that the sexual attractiveness of 
      urine from castrated males to females significantly increased after the addition 
      of either recombinant OBP3 (rOBP3) or recombinant MUP13 (rMUP13). Using c-Fos 
      immunohistochemistry, we further examined neuronal activation in the brains of 
      female rats after they sniffed rOBP3 or rMUP13. Both rOBP3 and rMUP13 activated 
      the accessory olfactory bulb (AOB), medial preoptic area (MPA), bed nucleus of 
      the stria terminalis (BST), medial amygdala (MeA), posteromedial cortical 
      amygdala (PMCo) and ventromedial nucleus of the hypothalamus (VMH), which 
      participate in the neural circuits responsible for pheromone-induced sexual 
      behaviours. In particular, more c-Fos-immunopositive (c-Fos-ir) cells were 
      observed in the posterior AOB than in the anterior AOB. CONCLUSIONS: The 
      expression of OBP3 and MUP13 was male-biased and androgen-dependent. They 
      attracted females and activated brain areas related to sexual behaviours in 
      female rats, suggesting that both OBP3 and MUP13 are male pheromones in 
      rats. Particularly, an OBP excreted into urine was exemplified to be a chemical 
      signal.
FAU - Guo, Xiao
AU  - Guo X
AD  - 1State Key Laboratory of Integrated Management of Pest Insects and Rodents in 
      Agriculture, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichen West 
      Road, Beijing, 100101 China. ISNI: 0000 0004 1792 6416. GRID: grid.458458.0
AD  - 2University of Chinese Academy of Sciences, Beijing, 100049 China. ISNI: 0000 
      0004 1797 8419. GRID: grid.410726.6
FAU - Guo, Huifen
AU  - Guo H
AD  - 1State Key Laboratory of Integrated Management of Pest Insects and Rodents in 
      Agriculture, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichen West 
      Road, Beijing, 100101 China. ISNI: 0000 0004 1792 6416. GRID: grid.458458.0
FAU - Zhao, Lei
AU  - Zhao L
AD  - 1State Key Laboratory of Integrated Management of Pest Insects and Rodents in 
      Agriculture, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichen West 
      Road, Beijing, 100101 China. ISNI: 0000 0004 1792 6416. GRID: grid.458458.0
AD  - 2University of Chinese Academy of Sciences, Beijing, 100049 China. ISNI: 0000 
      0004 1797 8419. GRID: grid.410726.6
FAU - Zhang, Yao-Hua
AU  - Zhang YH
AUID- ORCID: 0000-0002-6318-413X
AD  - 1State Key Laboratory of Integrated Management of Pest Insects and Rodents in 
      Agriculture, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichen West 
      Road, Beijing, 100101 China. ISNI: 0000 0004 1792 6416. GRID: grid.458458.0
FAU - Zhang, Jian-Xu
AU  - Zhang JX
AD  - 1State Key Laboratory of Integrated Management of Pest Insects and Rodents in 
      Agriculture, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichen West 
      Road, Beijing, 100101 China. ISNI: 0000 0004 1792 6416. GRID: grid.458458.0
LA  - eng
PT  - Journal Article
DEP - 20180223
PL  - England
TA  - Front Zool
JT  - Frontiers in zoology
JID - 101231669
PMC - PMC5824612
OTO - NOTNLM
OT  - Activation of neural pathways
OT  - Female attraction
OT  - MUPs
OT  - Male pheromones
COIS- The procedures for animal care and use were performed in strict accordance with 
      the guidelines of the Animal Use Committee of the Institute of Zoology, Chinese 
      Academy of Sciences (IOZ 2015).Not applicable.The authors declare that they have 
      no competing interests.Springer Nature remains neutral with regard to 
      jurisdictional claims in published maps and institutional affiliations.
EDAT- 2018/02/28 06:00
MHDA- 2018/02/28 06:01
PMCR- 2018/02/23
CRDT- 2018/02/28 06:00
PHST- 2017/08/08 00:00 [received]
PHST- 2018/02/02 00:00 [accepted]
PHST- 2018/02/28 06:00 [entrez]
PHST- 2018/02/28 06:00 [pubmed]
PHST- 2018/02/28 06:01 [medline]
PHST- 2018/02/23 00:00 [pmc-release]
AID - 254 [pii]
AID - 10.1186/s12983-018-0254-0 [doi]
PST - epublish
SO  - Front Zool. 2018 Feb 23;15:6. doi: 10.1186/s12983-018-0254-0. eCollection 2018.