PMID- 29484382
OWN - NLM
STAT- MEDLINE
DCOM- 20180827
LR  - 20181202
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 41
IP  - 5
DP  - 2018 May
TI  - Human umbilical cord mesenchymal stem cells inhibit proliferation of hepatic 
      stellate cells in vitro.
PG  - 2545-2552
LID - 10.3892/ijmm.2018.3500 [doi]
AB  - The effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) on the 
      proliferation of hepatic stellate cells (HSCs) is largely unknown. The purpose of 
      this study was to explore the mechanism of action of hUC‑MSCs on the 
      proliferation of HSCs in vitro. The upper and lower double-cell co-culture system 
      was established between hUC‑MSCs and HSCs in the experimental group. HSCs were 
      cultured alone as a negative control group. Cell proliferation and apoptosis were 
      determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 
      assay and flow cytometry, respectively. Cell supernatants were harvested to 
      determine the concentration of transforming growth factor-beta1 (TGF-beta1) by ELISA. 
      mRNA and protein of TGF-beta1, Smad3 and Smad7 in HSCs were determined by reverse 
      transcription-polymerase chain reaction and western blotting, respectively. In 
      the co-culture group, the proliferation of HSCs was significantly inhibited 
      compared with the negative control group at 24 and 48 h (p<0.05). Apoptosis of 
      HSCs in the co-culture group increased compared with that in the negative control 
      group, which was more obvious at 48 h (p<0.05). The concentration of TGF-beta1 in 
      the co-culture group was significantly lower than in the HSCs cultured alone 
      (p<0.05). After HSCs were co-cultured with hUC‑MSCs for 48 h, expression of 
      TGF-beta1 and Smad3 mRNA and protein was reduced and expression of Smad7 mRNA and 
      protein was increased compared with the negative control group (p<0.05). hUC‑MSCs 
      inhibited proliferation of HSCs, possibly through inhibiting TGF-beta1 and Smad3 
      expression and increasing Smad7 protein expression.
FAU - Zhang, Li-Ting
AU  - Zhang LT
AD  - Department of Infectious Diseases, The First Hospital of Lanzhou University, 
      Lanzhou, Gansu 730000, P.R. China.
FAU - Peng, Xue-Bin
AU  - Peng XB
AD  - Department of Infectious Diseases, The First Hospital of Lanzhou University, 
      Lanzhou, Gansu 730000, P.R. China.
FAU - Fang, Xue-Qin
AU  - Fang XQ
AD  - Department of Infectious Diseases, The Central Hospital of Baoji, Baoji, Shannxi 
      721008, P.R. China.
FAU - Li, Jun-Feng
AU  - Li JF
AD  - Department of Infectious Diseases, The First Hospital of Lanzhou University, 
      Lanzhou, Gansu 730000, P.R. China.
FAU - Chen, Hong
AU  - Chen H
AD  - Department of Infectious Diseases, The First Hospital of Lanzhou University, 
      Lanzhou, Gansu 730000, P.R. China.
FAU - Mao, Xiao-Rong
AU  - Mao XR
AD  - Department of Infectious Diseases, The First Hospital of Lanzhou University, 
      Lanzhou, Gansu 730000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180216
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (SMAD3 protein, human)
RN  - 0 (SMAD7 protein, human)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad7 Protein)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Apoptosis
MH  - Cell Line
MH  - *Cell Proliferation
MH  - Coculture Techniques
MH  - Gene Expression Regulation
MH  - Hepatic Stellate Cells/*cytology/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Smad3 Protein/analysis/genetics
MH  - Smad7 Protein/analysis/genetics
MH  - Transforming Growth Factor beta1/analysis/genetics
MH  - Umbilical Cord/*cytology/metabolism
PMC - PMC5846650
COIS- Competing interests The authors declare that they have no competing interests.
EDAT- 2018/02/28 06:00
MHDA- 2018/08/28 06:00
PMCR- 2018/02/16
CRDT- 2018/02/28 06:00
PHST- 2016/07/01 00:00 [received]
PHST- 2018/01/22 00:00 [accepted]
PHST- 2018/02/28 06:00 [pubmed]
PHST- 2018/08/28 06:00 [medline]
PHST- 2018/02/28 06:00 [entrez]
PHST- 2018/02/16 00:00 [pmc-release]
AID - ijmm-41-05-2545 [pii]
AID - 10.3892/ijmm.2018.3500 [doi]
PST - ppublish
SO  - Int J Mol Med. 2018 May;41(5):2545-2552. doi: 10.3892/ijmm.2018.3500. Epub 2018 
      Feb 16.