PMID- 29484389
OWN - NLM
STAT- MEDLINE
DCOM- 20180830
LR  - 20211204
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 17
IP  - 4
DP  - 2018 Apr
TI  - mTOR is involved in stroke-induced seizures and the anti-seizure effect of mild 
      hypothermia.
PG  - 5821-5829
LID - 10.3892/mmr.2018.8629 [doi]
AB  - Stroke is considered an underlying etiology of the development of seizures. 
      Stroke leads to glucose and oxygen deficiency in neurons, resulting in brain 
      dysfunction and injury. Mild hypothermia is a therapeutic strategy to inhibit 
      stroke‑induced seizures, which may be associated with the regulation of energy 
      metabolism of the brain. Mammalian target of rapamycin (mTOR) signaling and 
      solute carrier family 2, facilitated glucose transporter member (GLUT)‑1 are 
      critical for energy metabolism. Furthermore, mTOR overactivation and GLUT‑1 
      deficiency are associated with genetically acquired seizures. It has been 
      hypothesized that mTOR and GLUT‑1 may additionally be involved in seizures 
      elicited by stroke. The present study established global cerebral ischemia (GCI) 
      models of rats. Convulsive seizure behaviors frequently occurred during the first 
      and the second days following GCI, which were accompanied with seizure discharge 
      reflected in the EEG monitor. Expression of phosphor (p)‑mTOR and GLUT‑1 were 
      upregulated in the cerebral cortex and hippocampus, as evidenced by 
      immunohistochemistry and western blot analyses. Mild hypothermia and/or rapamycin 
      (mTOR inhibitor) treatments reduced the number of epileptic attacks, seizure 
      severity scores and seizure discharges, thereby alleviating seizures induced by 
      GCI. Mild hypothermia and/or rapamycin treatments reduced phosphorylation levels 
      of mTOR and the downstream effecter p70S6 in neurons, and the amount of GLUT‑1 in 
      the cytomembrane of neurons. The present study revealed that mTOR is involved in 
      stroke‑induced seizures and the anti‑seizure effect of mild hypothermia. The role 
      of GLUT‑1 in stroke‑elicited seizures appears to be different from the role in 
      seizures induced by other reasons. Further studies are necessary in order to 
      elucidate the exact function of GLUT-1 in stroke‑elicited seizures.
FAU - Yang, Guo-Shuai
AU  - Yang GS
AD  - Department of Neurology, Affiliated Haikou Hospital, Xiangya School of Medicine, 
      Central South University, Haikou, Hainan 570208, P.R. China.
FAU - Zhou, Xiao-Yan
AU  - Zhou XY
AD  - Department of Neurology, Affiliated Haikou Hospital, Xiangya School of Medicine, 
      Central South University, Haikou, Hainan 570208, P.R. China.
FAU - An, Xue-Fang
AU  - An XF
AD  - Department of Neurology, Affiliated Haikou Hospital, Xiangya School of Medicine, 
      Central South University, Haikou, Hainan 570208, P.R. China.
FAU - Liu, Xuan-Jun
AU  - Liu XJ
AD  - Department of Neurology, Affiliated Haikou Hospital, Xiangya School of Medicine, 
      Central South University, Haikou, Hainan 570208, P.R. China.
FAU - Zhang, Yan-Jun
AU  - Zhang YJ
AD  - Department of Neurology, Affiliated Haikou Hospital, Xiangya School of Medicine, 
      Central South University, Haikou, Hainan 570208, P.R. China.
FAU - Yu, Dan
AU  - Yu D
AD  - Department of Neurology, Affiliated Haikou Hospital, Xiangya School of Medicine, 
      Central South University, Haikou, Hainan 570208, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180222
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Biomarkers)
RN  - 0 (Glucose Transporter Type 1)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - IY9XDZ35W2 (Glucose)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Brain Ischemia/complications
MH  - Disease Models, Animal
MH  - Electroencephalography
MH  - Glucose/metabolism
MH  - Glucose Transporter Type 1/metabolism
MH  - *Hypothermia, Induced/methods
MH  - Immunohistochemistry
MH  - Male
MH  - Neurons/metabolism
MH  - Rats
MH  - Seizures/diagnosis/*etiology/*metabolism/therapy
MH  - Severity of Illness Index
MH  - Signal Transduction/drug effects
MH  - Sirolimus/pharmacology
MH  - Stroke/*complications
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC5866026
OTO - NOTNLM
OT  - mammalian target of rapamycin
OT  - stroke
OT  - seizures
OT  - mild hypothermia
OT  - solute carrier family 2 facilitated glucose transporter member 1
EDAT- 2018/02/28 06:00
MHDA- 2018/08/31 06:00
PMCR- 2018/02/22
CRDT- 2018/02/28 06:00
PHST- 2017/08/07 00:00 [received]
PHST- 2017/10/05 00:00 [accepted]
PHST- 2018/02/28 06:00 [pubmed]
PHST- 2018/08/31 06:00 [medline]
PHST- 2018/02/28 06:00 [entrez]
PHST- 2018/02/22 00:00 [pmc-release]
AID - mmr-17-04-5821 [pii]
AID - 10.3892/mmr.2018.8629 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Apr;17(4):5821-5829. doi: 10.3892/mmr.2018.8629. Epub 2018 Feb 
      22.