PMID- 29484729
OWN - NLM
STAT- MEDLINE
DCOM- 20180820
LR  - 20201209
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 32
IP  - 6
DP  - 2018 Jun
TI  - Pinus densiflora bark extract ameliorates 2,4-dinitrochlorobenzene-induced atopic 
      dermatitis in NC/Nga mice by regulating Th1/Th2 balance and skin barrier 
      function.
PG  - 1135-1143
LID - 10.1002/ptr.6061 [doi]
AB  - Korean red pine (Pinus densiflora) bark has been traditionally used in Korea and 
      other parts of East Asia to relieve inflammatory diseases. Although many studies 
      using P. densiflora bark have been reported, its effect on atopic dermatitis (AD) 
      has not been elucidated. Thus, we investigated whether the P. densiflora bark 
      extract (PBE) has potential to attenuate AD symptoms and elucidated the molecular 
      mechanism. Oral administration of PBE to mice with 2,4-dinitrochlorobenzene 
      (DNCB)-induced AD lessened dermatitis scores and scratching behavior and 
      significantly reduced measures of epidermal thickness, infiltration of mast cells 
      and eosinophils, levels of immunoglobulin E (IgE), and IgG(1) /IgG(2a) ratio in 
      serum. PBE not only inhibited IL-4, IL-5, and IL-13 but also increased IFN-gamma in 
      splenic production. Furthermore, PBE significantly suppressed mRNA expression of 
      thymic stromal lymphopoietin and further downregulated the mRNA expression of Th2 
      and Th17 cytokines such as IL-4, IL-13, IL-17, IL-31, and TNF-alpha. In addition, the 
      protein expressions of filaggrin, involucrin, and loricrin in lesional skin were 
      recovered by PBE. These results suggest that PBE attenuates DNCB-induced AD via 
      regulating Th1/Th2 balance and skin barrier function.
CI  - Copyright (c) 2018 John Wiley & Sons, Ltd.
FAU - Lee, Jun Woo
AU  - Lee JW
AUID- ORCID: 0000-0003-1716-3386
AD  - Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee 
      University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.
FAU - Wu, Qianwen
AU  - Wu Q
AUID- ORCID: 0000-0002-7475-3457
AD  - Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee 
      University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.
FAU - Jang, Young Pyo
AU  - Jang YP
AUID- ORCID: 0000-0001-5865-9228
AD  - Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee 
      University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.
AD  - Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee 
      University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.
FAU - Choung, Se Young
AU  - Choung SY
AUID- ORCID: 0000-0001-7619-6263
AD  - Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee 
      University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.
AD  - Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee 
      University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20180227
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (Dinitrochlorobenzene)
SB  - IM
MH  - Animals
MH  - Dermatitis, Atopic/*chemically induced
MH  - Dinitrochlorobenzene/*adverse effects
MH  - Male
MH  - Mice
MH  - Skin/*drug effects
MH  - Th1-Th2 Balance/*genetics
OTO - NOTNLM
OT  - NC/Nga mice
OT  - Pinus densiflora
OT  - Th1
OT  - Th2
OT  - atopic dermatitis
OT  - filaggrin
EDAT- 2018/02/28 06:00
MHDA- 2018/08/21 06:00
CRDT- 2018/02/28 06:00
PHST- 2017/11/20 00:00 [received]
PHST- 2018/01/05 00:00 [revised]
PHST- 2018/01/17 00:00 [accepted]
PHST- 2018/02/28 06:00 [pubmed]
PHST- 2018/08/21 06:00 [medline]
PHST- 2018/02/28 06:00 [entrez]
AID - 10.1002/ptr.6061 [doi]
PST - ppublish
SO  - Phytother Res. 2018 Jun;32(6):1135-1143. doi: 10.1002/ptr.6061. Epub 2018 Feb 27.