PMID- 29486535
OWN - NLM
STAT- MEDLINE
DCOM- 20180918
LR  - 20181113
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 19
IP  - 3
DP  - 2018 May 31
TI  - Phenotypic and genotypic analyses of an attenuated porcine reproductive and 
      respiratory syndrome virus strain after serial passages in cultured porcine 
      alveolar macrophages.
PG  - 358-367
LID - 10.4142/jvs.2018.19.3.358 [doi]
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) is a globally 
      ubiquitous swine viral pathogen that causes major economic losses worldwide. We 
      previously reported an over-attenuated phenotype of cell-adapted PRRSV strain 
      CA-2-P100 in vivo. In the present study, CA-2-P100 was serially propagated in 
      cultured porcine alveolar macrophage (PAM) cells for up to 20 passages to obtain 
      the derivative strain CA-2-MP120. Animal inoculation studies revealed that both 
      CA-2-P100 and CA-2-MP120 had decreased virulence, eliciting weight gains, body 
      temperatures, and histopathologic lesions similar to those in the negative 
      control group. However, compared to CA-2-P100 infection, CA-2-MP120 yielded 
      consistently higher viremia kinetics and enhanced antibody responses in pigs. All 
      pigs inoculated with CA-2-MP120 developed viremia and seroconverted to PRRSV. 
      During 20 passages in PAM cells, CA-2-MP120 acquired 15 amino acid changes that 
      were mostly distributed in nsp2 and minor structural protein-coding regions. 
      Among these changes, 6 mutations represented reversions to the sequences of the 
      reference CA-2 and parental CA-2-P20 strains. These genetic drifts may be 
      hypothetical molecular markers associated with PRRSV macrophage tropism and 
      virulence. Our results indicate that the PAM-passaged CA-2-MP120 strain is a 
      potential candidate for developing a live, attenuated PRRSV vaccine.
FAU - Lee, Seung-Chul
AU  - Lee SC
AD  - Choongang Vaccine Laboratory, Daejeon 34055, Korea.
AD  - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, 
      Korea.
FAU - Lee, Sunhee
AU  - Lee S
AD  - Animal Virology Laboratory, School of Life Sciences, BK21 Plus KNU Creative 
      BioResearch Group, Kyungpook National University, Daegu 41566, Korea.
FAU - Yoo, Gun-Woo
AU  - Yoo GW
AD  - Choongang Vaccine Laboratory, Daejeon 34055, Korea.
FAU - Choi, Hwan-Won
AU  - Choi HW
AD  - Choongang Vaccine Laboratory, Daejeon 34055, Korea.
FAU - Noh, Yun-Hee
AU  - Noh YH
AD  - Choongang Vaccine Laboratory, Daejeon 34055, Korea.
FAU - Park, Chang Eon
AU  - Park CE
AD  - School of Applied Biosciences, College of Agriculture and Life Sciences, 
      Kyungpook National University, Daegu 41566, Korea.
FAU - Shin, Jae-Ho
AU  - Shin JH
AD  - School of Applied Biosciences, College of Agriculture and Life Sciences, 
      Kyungpook National University, Daegu 41566, Korea.
FAU - Yoon, In-Joong
AU  - Yoon IJ
AD  - Choongang Vaccine Laboratory, Daejeon 34055, Korea.
FAU - Kang, Shien-Young
AU  - Kang SY
AD  - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, 
      Korea.
FAU - Lee, Changhee
AU  - Lee C
AD  - Animal Virology Laboratory, School of Life Sciences, BK21 Plus KNU Creative 
      BioResearch Group, Kyungpook National University, Daegu 41566, Korea.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - *Genotype
MH  - Immunogenicity, Vaccine
MH  - Macrophages, Alveolar/*virology
MH  - *Phenotype
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - Serial Passage/veterinary
MH  - Swine
MH  - Virulence
PMC - PMC5974517
OTO - NOTNLM
OT  - attenuated vaccines
OT  - macrophage tropism
OT  - porcine reproductive and respiratory syndrome virus
OT  - virulence
OT  - whole genome sequencing
COIS- Conflict of Interest: The authors declare no conflicts of interest.
EDAT- 2018/02/28 06:00
MHDA- 2018/09/19 06:00
PMCR- 2018/05/01
CRDT- 2018/02/28 06:00
PHST- 2017/10/20 00:00 [received]
PHST- 2018/01/24 00:00 [revised]
PHST- 2018/01/30 00:00 [accepted]
PHST- 2018/02/28 06:00 [pubmed]
PHST- 2018/09/19 06:00 [medline]
PHST- 2018/02/28 06:00 [entrez]
PHST- 2018/05/01 00:00 [pmc-release]
AID - jvs.18023 [pii]
AID - 10.4142/jvs.2018.19.3.358 [doi]
PST - ppublish
SO  - J Vet Sci. 2018 May 31;19(3):358-367. doi: 10.4142/jvs.2018.19.3.358.