PMID- 29487295
OWN - NLM
STAT- MEDLINE
DCOM- 20190923
LR  - 20190923
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Feb 27
TI  - Evidences for a New Role of miR-214 in Chondrogenesis.
PG  - 3704
LID - 10.1038/s41598-018-21735-w [doi]
LID - 3704
AB  - miR-214 is known to play a role in mammalian skeletal development through 
      inhibition of osteogenesis and stimulation of osteoclastogenesis, but data 
      regarding other vertebrates, as well as a possible role in chondrogenesis, remain 
      unknown. Here, we show that miR-214 expression is detected in bone and cartilage 
      of zebrafish skeleton, and is downregulated during murine ATDC5 chondrocyte 
      differentiation. Additionally, we observed a conservation of the transcriptional 
      regulation of miR-214 primary transcript Dnm3os in vertebrates, being regulated 
      by Ets1 in ATDC5 chondrogenic cells. Moreover, overexpression of miR-214 in vitro 
      and in vivo mitigated chondrocyte differentiation probably by targeting 
      activating transcription factor 4 (Atf4). Indeed, miR-214 overexpression in vivo 
      hampered cranial cartilage formation of zebrafish and coincided with 
      downregulation of atf4 and of the key chondrogenic players sox9 and col2a1. We 
      show that miR-214 overexpression exerts a negative role in chondrogenesis by 
      impacting on chondrocyte differentiation possibly through conserved mechanisms.
FAU - Roberto, Vania Palma
AU  - Roberto VP
AD  - Centre of Marine Sciences (CCMAR/CIMAR-LA), University of Algarve, 8005-139, 
      Faro, Portugal.
AD  - PhD Program in Biomedical Sciences, DCBM, University of Algarve, 8005-139, Faro, 
      Portugal.
AD  - Algarve Biomedical Center, University of Algarve, Campus de Gambelas, 8005-139, 
      Faro, Portugal.
AD  - Department of Biomedical Sciences and Medicine, University of Algarve, 8005-139, 
      Faro, Portugal.
FAU - Gavaia, Paulo
AU  - Gavaia P
AD  - Centre of Marine Sciences (CCMAR/CIMAR-LA), University of Algarve, 8005-139, 
      Faro, Portugal.
AD  - Department of Biomedical Sciences and Medicine, University of Algarve, 8005-139, 
      Faro, Portugal.
FAU - Nunes, Maria Joao
AU  - Nunes MJ
AD  - Instituto de Investigacao do Medicamento (iMed.ULisboa), Faculty of Pharmacy, 
      Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
FAU - Rodrigues, Elsa
AU  - Rodrigues E
AD  - Instituto de Investigacao do Medicamento (iMed.ULisboa), Faculty of Pharmacy, 
      Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
AD  - Department of Biochemistry and Human Biology, Faculty of Pharmacy, Universidade 
      de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
FAU - Cancela, Maria Leonor
AU  - Cancela ML
AD  - Centre of Marine Sciences (CCMAR/CIMAR-LA), University of Algarve, 8005-139, 
      Faro, Portugal. lcancela@ualg.pt.
AD  - Algarve Biomedical Center, University of Algarve, Campus de Gambelas, 8005-139, 
      Faro, Portugal. lcancela@ualg.pt.
AD  - Department of Biomedical Sciences and Medicine, University of Algarve, 8005-139, 
      Faro, Portugal. lcancela@ualg.pt.
FAU - Tiago, Daniel Martins
AU  - Tiago DM
AD  - Centre of Marine Sciences (CCMAR/CIMAR-LA), University of Algarve, 8005-139, 
      Faro, Portugal. dtiago@ualg.pt.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180227
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (MicroRNAs)
RN  - 0 (Zebrafish Proteins)
SB  - IM
MH  - Animals
MH  - Cartilage/metabolism
MH  - Cell Differentiation/genetics/physiology
MH  - Chondrocytes/cytology/metabolism
MH  - Chondrogenesis/genetics/*physiology
MH  - Gene Expression Regulation/genetics/physiology
MH  - Mice
MH  - MicroRNAs/genetics/*metabolism
MH  - Zebrafish
MH  - Zebrafish Proteins/genetics/metabolism
PMC - PMC5829070
COIS- The authors declare no competing interests.
EDAT- 2018/03/01 06:00
MHDA- 2019/09/24 06:00
PMCR- 2018/02/27
CRDT- 2018/03/01 06:00
PHST- 2017/08/02 00:00 [received]
PHST- 2018/02/08 00:00 [accepted]
PHST- 2018/03/01 06:00 [entrez]
PHST- 2018/03/01 06:00 [pubmed]
PHST- 2019/09/24 06:00 [medline]
PHST- 2018/02/27 00:00 [pmc-release]
AID - 10.1038/s41598-018-21735-w [pii]
AID - 21735 [pii]
AID - 10.1038/s41598-018-21735-w [doi]
PST - epublish
SO  - Sci Rep. 2018 Feb 27;8(1):3704. doi: 10.1038/s41598-018-21735-w.