PMID- 29488195
OWN - NLM
STAT- MEDLINE
DCOM- 20180829
LR  - 20181202
IS  - 1530-9932 (Electronic)
IS  - 1530-9932 (Linking)
VI  - 19
IP  - 4
DP  - 2018 May
TI  - Encapsulation in Polymeric Microparticles Improves Daptomycin Activity Against 
      Mature Staphylococci Biofilms-a Thermal and Imaging Study.
PG  - 1625-1636
LID - 10.1208/s12249-018-0974-7 [doi]
AB  - Eradication of Gram-positive biofilms is a critical aspect in implant-associated 
      infection treatment. Although antibiotic-containing particulate carriers may be a 
      promising strategy for overcoming biofilm tolerance, the assessment of their 
      interaction with biofilms has not been fully explored. In the present work, the 
      antibiofilm activity of daptomycin- and vancomycin-loaded poly(methyl 
      methacrylate) (PMMA) and PMMA-Eudragit RL 100 (EUD) microparticles against 
      methicillin-resistant Staphylococcus aureus (MRSA) and polysaccharide 
      intercellular adhesin-positive S. epidermidis biofilms was investigated using 
      isothermal microcalorimetry (IMC) and fluorescence in situ hybridization (FISH). 
      The minimal biofilm inhibitory concentrations (MBIC) of MRSA biofilms, as 
      determined by IMC, were 5 and 20 mg/mL for daptomycin- and vancomycin-loaded PMMA 
      microparticles, respectively. S. epidermidis biofilms were less susceptible, with 
      a MBIC of 20 mg/mL for daptomycin-loaded PMMA microparticles. Vancomycin-loaded 
      microparticles were ineffective. Adding EUD to the formulation caused a 4- and 
      16-fold reduction of the MBIC values of daptomycin-loaded microparticles for S. 
      aureus and S. epidermidis, respectively. FISH corroborated the IMC results and 
      provided additional insights on the antibiofilm effect of these particles. 
      According to microscopic analysis, only daptomycin-loaded PMMA-EUD microparticles 
      were causing a pronounced reduction in biofilm mass for both strains. Taken 
      together, although IMC indicated that a biofilm inhibition was achieved, 
      microscopy showed that the biofilm was not eradicated and still contained 
      FISH-positive, presumably viable bacteria, thus indicating that combining the two 
      techniques is essential to fully assess the effect of microparticles on 
      staphylococcal biofilms.
FAU - Santos Ferreira, Ines
AU  - Santos Ferreira I
AD  - Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmacia, 
      Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
FAU - Kikhney, Judith
AU  - Kikhney J
AD  - Biofilmcenter, Deutsches Herzzentrum Berlin, Charite-Universitatsmedizin Berlin, 
      Hindenburgdamm 30, 12203, Berlin, Germany.
AD  - Institute for Microbiology and Hygiene, Charite-Universitatsmedizin Berlin, 
      Hindenburgdamm 30, 12203, Berlin, Germany.
FAU - Kursawe, Laura
AU  - Kursawe L
AD  - Biofilmcenter, Deutsches Herzzentrum Berlin, Charite-Universitatsmedizin Berlin, 
      Hindenburgdamm 30, 12203, Berlin, Germany.
FAU - Kasper, Stefanie
AU  - Kasper S
AD  - Biofilmcenter, Deutsches Herzzentrum Berlin, Charite-Universitatsmedizin Berlin, 
      Hindenburgdamm 30, 12203, Berlin, Germany.
FAU - Goncalves, Lidia M D
AU  - Goncalves LMD
AD  - Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmacia, 
      Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
FAU - Trampuz, Andrej
AU  - Trampuz A
AD  - Center for Musculoskeletal Surgery, Charite-Universitatsmedizin Berlin, Free and 
      Humboldt-University of Berlin, Chariteplatz 1, 10117, Berlin, Germany.
FAU - Moter, Annette
AU  - Moter A
AD  - Biofilmcenter, Deutsches Herzzentrum Berlin, Charite-Universitatsmedizin Berlin, 
      Hindenburgdamm 30, 12203, Berlin, Germany.
FAU - Bettencourt, Ana Francisca
AU  - Bettencourt AF
AD  - Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmacia, 
      Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal. 
      asimao@ff.ulisboa.pt.
FAU - Almeida, Antonio J
AU  - Almeida AJ
AD  - Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmacia, 
      Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal. 
      aalmeida@ff.ulisboa.pt.
LA  - eng
PT  - Journal Article
DEP - 20180227
PL  - United States
TA  - AAPS PharmSciTech
JT  - AAPS PharmSciTech
JID - 100960111
RN  - 0 (Anti-Bacterial Agents)
RN  - NWQ5N31VKK (Daptomycin)
SB  - IM
MH  - Anti-Bacterial Agents/administration & dosage/chemistry/metabolism
MH  - Biofilms/*drug effects/growth & development
MH  - Daptomycin/administration & dosage/*chemistry/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Methicillin-Resistant Staphylococcus aureus/*drug effects/physiology
MH  - Microbial Sensitivity Tests/methods
MH  - *Microspheres
MH  - Staphylococcus epidermidis/*drug effects/physiology
OTO - NOTNLM
OT  - daptomycin
OT  - fluorescence in situ hybridization
OT  - isothermal microcalorimetry
OT  - microencapsulation
OT  - staphylococcal biofilms
EDAT- 2018/03/01 06:00
MHDA- 2018/08/30 06:00
CRDT- 2018/03/01 06:00
PHST- 2017/11/08 00:00 [received]
PHST- 2018/02/11 00:00 [accepted]
PHST- 2018/03/01 06:00 [pubmed]
PHST- 2018/08/30 06:00 [medline]
PHST- 2018/03/01 06:00 [entrez]
AID - 10.1208/s12249-018-0974-7 [pii]
AID - 10.1208/s12249-018-0974-7 [doi]
PST - ppublish
SO  - AAPS PharmSciTech. 2018 May;19(4):1625-1636. doi: 10.1208/s12249-018-0974-7. Epub 
      2018 Feb 27.