PMID- 29488424
OWN - NLM
STAT- MEDLINE
DCOM- 20181221
LR  - 20181221
IS  - 1563-5279 (Electronic)
IS  - 0020-7454 (Linking)
VI  - 128
IP  - 10
DP  - 2018 Oct
TI  - Time- and dose-related effects of amyloid beta1-40 on retina and optic nerve 
      morphology in rats.
PG  - 952-965
LID - 10.1080/00207454.2018.1446953 [doi]
AB  - PURPOSE: Amyloid beta (Abeta) is known to contribute to the pathophysiology of 
      retinal neurodegenerative diseases such as glaucoma. Effects of intravitreal 
      Abeta(1-42) on retinal and optic nerve morphology in animal models have widely been 
      studied but not those of Abeta(1-40). Hence, we evaluated the time- and dose-related 
      effects of intravitreal Abeta(1-40) on retinal and optic nerve morphology. Since 
      oxidative stress and brain derived neurotrophic factor (BDNF) are associated with 
      Abeta-induced neuronal damage, we also studied dose and time-related effects of 
      Abeta(1-40) on retinal oxidative stress and BDNF levels. MATERIALS AND METHODS: Five 
      groups of rats were intravitreally administered with vehicle or Abeta(1-40) in doses 
      of 1.0, 2.5, 5 and 10 nmol. Animals were sacrificed and eyes were enucleated at 
      weeks 1, 2 and 4 post-injection. The retinae were subjected to morphometric 
      analysis and TUNEL staining. Optic nerve sections were stained with toluidine 
      blue and were graded for neurodegenerative effects. The estimation of BDNF and 
      markers of oxidative stress in retina were done using ELISA technique. RESULTS 
      AND CONCLUSIONS: It was observed that intravitreal Abeta(1-40) causes significant 
      retinal and optic nerve damage up to day 14 post-injection and there was 
      increasing damage with increase in dose. However, on day 30 post-injection both 
      the retinal and optic nerve morphology showed a trend towards normalization. The 
      observations made for retinal cell apoptosis, retinal glutathione, superoxide 
      dismutase activity and BDNF were in accordance with those of morphological 
      changes with deterioration till day 14 and recovery by day 30 post-injection. The 
      findings of this study may provide a guide for selection of appropriate 
      experimental conditions for future studies.
FAU - Mohd Lazaldin, Mohd Aizuddin
AU  - Mohd Lazaldin MA
AUID- ORCID: 0000-0002-7727-6043
AD  - a Centre For Neuroscience Research, Faculty of Medicine , Universiti Teknologi 
      MARA, Sungai Buloh Campus, Selangor , Malaysia.
FAU - Iezhitsa, Igor
AU  - Iezhitsa I
AUID- ORCID: 0000-0002-2852-8486
AD  - a Centre For Neuroscience Research, Faculty of Medicine , Universiti Teknologi 
      MARA, Sungai Buloh Campus, Selangor , Malaysia.
AD  - b Research Institute of Pharmacology, Volgograd State Medical University , 
      Volgograd , Russia.
FAU - Agarwal, Renu
AU  - Agarwal R
AUID- ORCID: 0000-0002-3050-7449
AD  - a Centre For Neuroscience Research, Faculty of Medicine , Universiti Teknologi 
      MARA, Sungai Buloh Campus, Selangor , Malaysia.
FAU - Bakar, Nor Salmah
AU  - Bakar NS
AD  - a Centre For Neuroscience Research, Faculty of Medicine , Universiti Teknologi 
      MARA, Sungai Buloh Campus, Selangor , Malaysia.
FAU - Agarwal, Puneet
AU  - Agarwal P
AD  - c IMU Clinical School, International Medical University , Seremban , Malaysia.
FAU - Mohd Ismail, Nafeeza
AU  - Mohd Ismail N
AD  - a Centre For Neuroscience Research, Faculty of Medicine , Universiti Teknologi 
      MARA, Sungai Buloh Campus, Selangor , Malaysia.
LA  - eng
PT  - Journal Article
DEP - 20180320
PL  - England
TA  - Int J Neurosci
JT  - The International journal of neuroscience
JID - 0270707
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Bdnf protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Peptide Fragments)
RN  - 0 (amyloid beta-protein (1-40))
SB  - IM
MH  - Amyloid beta-Peptides/administration & dosage/*toxicity
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - In Situ Nick-End Labeling
MH  - *Optic Nerve/drug effects/metabolism/pathology
MH  - Oxidative Stress/*drug effects
MH  - Peptide Fragments/administration & dosage/*toxicity
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Retina/drug effects/metabolism/pathology
MH  - Vitreous Body/drug effects
OTO - NOTNLM
OT  - Amyloid-beta 1-40 peptide
OT  - hematoxylin-eosin staining
OT  - neurotoxicity
OT  - optic nerve
OT  - retina
OT  - toluidine blue staining
OT  - tunnel staining
EDAT- 2018/03/01 06:00
MHDA- 2018/12/24 06:00
CRDT- 2018/03/01 06:00
PHST- 2018/03/01 06:00 [pubmed]
PHST- 2018/12/24 06:00 [medline]
PHST- 2018/03/01 06:00 [entrez]
AID - 10.1080/00207454.2018.1446953 [doi]
PST - ppublish
SO  - Int J Neurosci. 2018 Oct;128(10):952-965. doi: 10.1080/00207454.2018.1446953. 
      Epub 2018 Mar 20.