PMID- 29488525
OWN - NLM
STAT- MEDLINE
DCOM- 20180925
LR  - 20180925
IS  - 1473-0189 (Electronic)
IS  - 1473-0189 (Linking)
VI  - 18
IP  - 7
DP  - 2018 Mar 27
TI  - Dynamic monitoring of transmembrane potential changes: a study of ion channels 
      using an electrical double layer-gated FET biosensor.
PG  - 1047-1056
LID - 10.1039/c7lc01305a [doi]
AB  - In this research, we have designed, fabricated and characterized an electrical 
      double layer (EDL)-gated AlGaN/GaN high electron mobility transistor (HEMT) 
      biosensor array to study the transmembrane potential changes of cells. The sensor 
      array platform is designed to detect and count circulating tumor cells (CTCs) of 
      colorectal cancer (CRC) and investigate cellular bioelectric signals. Using the 
      EDL FET biosensor platform, cellular responses can be studied in physiological 
      salt concentrations, thereby eliminating complex automation. Upon investigation, 
      we discovered that our sensor response follows the transmembrane potential 
      changes of captured cells. Our whole cell sensor platform can be used to monitor 
      the dynamic changes in the membrane potential of cells. The effects of 
      continuously changing electrolyte ion concentrations and ion channel blocking 
      using cadmium are investigated. This methodology has the potential to be used as 
      an electrophysiological probe for studying ion channel gating and the interaction 
      of biomolecules in cells. The sensor can also be a point-of-care diagnostic tool 
      for rapid screening of diseases.
FAU - Pulikkathodi, Anil Kumar
AU  - Pulikkathodi AK
AD  - Institute of NanoEngineering and MicroSystems, National Tsing Hua University, 
      Hsinchu, Taiwan 300, R.O.C. ylwang@mx.nthu.edu.tw gwobin@pme.nthu.edu.tw.
FAU - Sarangadharan, Indu
AU  - Sarangadharan I
FAU - Chen, Yi-Hong
AU  - Chen YH
FAU - Lee, Geng-Yen
AU  - Lee GY
FAU - Chyi, Jen-Inn
AU  - Chyi JI
FAU - Lee, Gwo-Bin
AU  - Lee GB
FAU - Wang, Yu-Lin
AU  - Wang YL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Ion Channels)
SB  - IM
MH  - *Biosensing Techniques
MH  - Cell Line
MH  - Colorectal Neoplasms/diagnosis/*metabolism
MH  - Electrons
MH  - Humans
MH  - Ion Channels/*metabolism
MH  - Neoplastic Cells, Circulating/*metabolism/pathology
MH  - Point-of-Care Testing
MH  - *Thermodynamics
EDAT- 2018/03/01 06:00
MHDA- 2018/09/27 06:00
CRDT- 2018/03/01 06:00
PHST- 2018/03/01 06:00 [pubmed]
PHST- 2018/09/27 06:00 [medline]
PHST- 2018/03/01 06:00 [entrez]
AID - 10.1039/c7lc01305a [doi]
PST - ppublish
SO  - Lab Chip. 2018 Mar 27;18(7):1047-1056. doi: 10.1039/c7lc01305a.