PMID- 29489343
OWN - NLM
STAT- MEDLINE
DCOM- 20190322
LR  - 20190322
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 19
IP  - 4
DP  - 2018 Apr 9
TI  - Impact of Bioinspired Nanotopography on the Antibacterial and Antibiofilm 
      Efficacy of Chitosan.
PG  - 1340-1346
LID - 10.1021/acs.biomac.8b00200 [doi]
AB  - Chitosan derived from chitin is one of the most abundant naturally occurring 
      biocompatible polymers obtained from fungi and arthropods. In this work, we 
      report the enhancement in the bactericidal efficacy of CHI in the presence of a 
      sharp nanotopography. High-aspect ratio nanostructured surface (NSS) was 
      fabricated using a single-step deep reactive ion etching technique (DRIE). Post 
      fabrication, CHI coating was carried out using a layer-by-layer (LBL) dip coating 
      process on the flat and nanostructured surfaces. Antibacterial efficacy of the 
      flat silicon surface coated with CHI (Si_CHI) and NSS coated with CHI (NSS_CHI) 
      was tested against both Gram-negative (G-ve) bacteria E. coli and Gram-positive 
      (G+ve) bacteria S. aureus. NSS_CHI exhibited superior antibacterial property 
      against G-ve and G+ve microbes as compared with Si_CHI and NSS substrates. 
      Scanning electron microscopy (SEM) and fluorescence microscopy were used to study 
      the morphology and viability of the bacteria on all the surfaces. Also, biofilm 
      quantification was carried out on all the engineered surfaces for both E. coli 
      and S. aureus using crystal violet (CV) staining. NSS_CHI was found to have the 
      minimum biofilm formation on its surface exhibiting its superior antibacterial 
      property. This study shows that the antibacterial and antibiofilm efficiency of 
      CHI can be augmented by combining it with a sharp nanotopography.
FAU - Tripathy, Abinash
AU  - Tripathy A
AUID- ORCID: 0000-0003-3546-2806
FAU - Pahal, Suman
AU  - Pahal S
FAU - Mudakavi, Rajeev J
AU  - Mudakavi RJ
AUID- ORCID: 0000-0001-8686-8561
FAU - Raichur, Ashok M
AU  - Raichur AM
AUID- ORCID: 0000-0001-5042-3122
FAU - Varma, Manoj M
AU  - Varma MM
FAU - Sen, Prosenjit
AU  - Sen P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180305
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Anti-Bacterial Agents)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Anti-Bacterial Agents/*chemistry/pharmacology
MH  - Biofilms/*drug effects
MH  - Chitosan/*chemistry/pharmacology
MH  - Escherichia coli/drug effects
MH  - Microscopy, Electron, Scanning
MH  - Nanostructures/*chemistry
MH  - Staphylococcus aureus/drug effects
MH  - Surface Properties
EDAT- 2018/03/01 06:00
MHDA- 2019/03/23 06:00
CRDT- 2018/03/01 06:00
PHST- 2018/03/01 06:00 [pubmed]
PHST- 2019/03/23 06:00 [medline]
PHST- 2018/03/01 06:00 [entrez]
AID - 10.1021/acs.biomac.8b00200 [doi]
PST - ppublish
SO  - Biomacromolecules. 2018 Apr 9;19(4):1340-1346. doi: 10.1021/acs.biomac.8b00200. 
      Epub 2018 Mar 5.