PMID- 29489438
OWN - NLM
STAT- MEDLINE
DCOM- 20181227
LR  - 20181227
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 31
IP  - 4
DP  - 2018 May
TI  - Isolation of Peripheral Blood CD8 T Cells Specific to Porcine Reproductive and 
      Respiratory Syndrome Virus Utilizing Porcine CD137 Activation Marker.
PG  - 333-337
LID - 10.1089/vim.2017.0189 [doi]
AB  - CD137 is a costimulatory molecule transiently expressed on activated T cells 
      after mitogen or antigen stimulation that can be exploited for isolating 
      antigen-specific T cells as reported in mouse models. By utilizing an antiporcine 
      CD137 monoclonal antibody (mAb, clone 3B9) developed in our laboratory, we 
      isolated virus-specific CD8beta T cells from peripheral blood of pigs experimentally 
      infected with different porcine reproductive and respiratory syndrome virus 
      (PRRSV) strains. Similar to mouse, porcine CD8beta T cells also express CD137 
      transiently upon Concavalin A stimulation while the unstimulated cells did not. 
      Most frequently, virus-specific CD8beta T cells were isolated at low levels from 
      peripheral blood of pigs experimentally infected with PRRSV strains VR2385, 
      NADC20, and MN184B at 49 and 63 days postinfection. The results suggest that 
      porcine CD137-specific mAb is a useful tool for isolating virus-specific CD8 T 
      cells from peripheral blood and tissues of pigs after in vitro stimulation with 
      viral antigen.
FAU - Subramaniam, Sakthivel
AU  - Subramaniam S
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University , 
      Blacksburg, Virginia.
FAU - Overend, Christopher
AU  - Overend C
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University , 
      Blacksburg, Virginia.
FAU - Yugo, Danielle M
AU  - Yugo DM
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University , 
      Blacksburg, Virginia.
FAU - Heffron, C Lynn
AU  - Heffron CL
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University , 
      Blacksburg, Virginia.
FAU - Matzinger, Shannon R
AU  - Matzinger SR
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University , 
      Blacksburg, Virginia.
FAU - Rogers, Adam J
AU  - Rogers AJ
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University , 
      Blacksburg, Virginia.
FAU - Tian, Debin
AU  - Tian D
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University , 
      Blacksburg, Virginia.
FAU - Cao, Qian M
AU  - Cao QM
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University , 
      Blacksburg, Virginia.
FAU - Kenney, Scott P
AU  - Kenney SP
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University , 
      Blacksburg, Virginia.
FAU - Meng, Xiang-Jin
AU  - Meng XJ
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University , 
      Blacksburg, Virginia.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20180228
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Tumor Necrosis Factor Receptor Superfamily, Member 9)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cell Line
MH  - HEK293 Cells
MH  - Humans
MH  - Lymphocyte Activation/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Tumor Necrosis Factor Receptor Superfamily, Member 9/*immunology
MH  - Viremia/immunology/veterinary/virology
OTO - NOTNLM
OT  - CD137
OT  - CD8 T cells
OT  - cell isolation
OT  - pig
OT  - porcine reproductive and respiratory syndrome virus
EDAT- 2018/03/01 06:00
MHDA- 2018/12/28 06:00
CRDT- 2018/03/01 06:00
PHST- 2018/03/01 06:00 [pubmed]
PHST- 2018/12/28 06:00 [medline]
PHST- 2018/03/01 06:00 [entrez]
AID - 10.1089/vim.2017.0189 [doi]
PST - ppublish
SO  - Viral Immunol. 2018 May;31(4):333-337. doi: 10.1089/vim.2017.0189. Epub 2018 Feb 
      28.