PMID- 29489935
OWN - NLM
STAT- MEDLINE
DCOM- 20180417
LR  - 20220331
IS  - 1678-7765 (Electronic)
IS  - 1678-7757 (Print)
IS  - 1678-7757 (Linking)
VI  - 26
DP  - 2018
TI  - Influence of Helicobacter pylori culture supernatant on the ecological balance of 
      a dual-species oral biofilm.
PG  - e20170113
LID - S1678-77572018000100427 [pii]
LID - 10.1590/1678-7757-2017-0113 [doi]
LID - e20170113
AB  - Dental caries is a chronic progressive disease occurring in the tooth hard tissue 
      due to multiple factors, in which bacteria are the initial cause. Both 
      Streptococcus mutans and Streptococcus sanguinis are main members of oral 
      biofilm. Helicobacter pylori may also be detected in dental plaque, playing an 
      important role in the development of dental caries. OBJECTIVE: The aim of this 
      study was to investigate the effect of H. pylori culture supernatant on S. mutans 
      and S. sanguinis dual-species biofilm and to evaluate its potential ability on 
      affecting dental health. MATERIAL AND METHODS: The effect of H. pylori 
      supernatant on single-species and dual-species biofilm was measured by colony 
      forming units counting and fluorescence in situ hybridization (FISH) assay, 
      respectively. The effect of H. pylori supernatant on S. mutans and S. sanguinis 
      extracellular polysaccharides (EPS) production was measured by both confocal 
      laser scanning microscopy observation and anthrone-sulfuric acid method. The 
      effect of H. pylori supernatant on S. mutans gene expression was measured by 
      quantitative real-time PCR (qRT-PCR) assays. RESULTS: H. pylori supernatant could 
      inhibit both S. mutans and S. sanguinis biofilm formation and EPS production. S. 
      sanguinis inhibition rate was significantly higher than that of S. mutans. 
      Finally, S. mutans bacteriocin and acidogenicity related genes expression were 
      affected by H. pylori culture supernatant. CONCLUSION: Our results showed that H. 
      pylori could destroy the balance between S. mutans and S. sanguinis in oral 
      biofilm, creating an advantageous environment for S. mutans, which became the 
      dominant bacteria, promoting the formation and development of dental caries.
FAU - Zhang, Wenling
AU  - Zhang W
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      People's Republic of China.
FAU - Deng, Xiaohong
AU  - Deng X
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      People's Republic of China.
FAU - Zhou, Xuedong
AU  - Zhou X
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      People's Republic of China.
FAU - Hao, Yuqing
AU  - Hao Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      People's Republic of China.
FAU - Li, Yuqing
AU  - Li Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20180222
PL  - Brazil
TA  - J Appl Oral Sci
JT  - Journal of applied oral science : revista FOB
JID - 101189774
RN  - 0 (Polysaccharides, Bacterial)
SB  - IM
MH  - *Biofilms
MH  - Colony Count, Microbial
MH  - Dental Caries/microbiology
MH  - Dental Plaque/*microbiology
MH  - Gene Expression
MH  - Helicobacter pylori/genetics/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - Plankton/growth & development
MH  - Polysaccharides, Bacterial/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Streptococcus mutans/genetics/*physiology
MH  - Streptococcus sanguis/genetics/*physiology
MH  - Time Factors
PMC - PMC5832010
EDAT- 2018/03/01 06:00
MHDA- 2018/04/18 06:00
PMCR- 2018/02/15
CRDT- 2018/03/01 06:00
PHST- 2017/03/10 00:00 [received]
PHST- 2017/07/01 00:00 [accepted]
PHST- 2018/03/01 06:00 [entrez]
PHST- 2018/03/01 06:00 [pubmed]
PHST- 2018/04/18 06:00 [medline]
PHST- 2018/02/15 00:00 [pmc-release]
AID - S1678-77572018000100427 [pii]
AID - 10.1590/1678-7757-2017-0113 [doi]
PST - ppublish
SO  - J Appl Oral Sci. 2018;26:e20170113. doi: 10.1590/1678-7757-2017-0113. Epub 2018 
      Feb 22.