PMID- 29491399 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20191120 IS - 2155-384X (Print) IS - 2155-384X (Electronic) IS - 2155-384X (Linking) VI - 9 IP - 3 DP - 2018 Feb 20 TI - Barrett's esophagus is associated with a distinct oral microbiome. PG - 135 LID - 10.1038/s41424-018-0005-8 [doi] LID - 135 AB - OBJECTIVES: The esophageal microbiome is composed of predominantly oral flora and is altered in reflux-related conditions including Barrett's esophagus (BE). Changes to the esophageal microbiome may be reflected in the oral cavity. Assessing the oral microbiome thus represents a potential non-invasive method to identify patients with BE. METHODS: Patients with and without BE undergoing upper endoscopy were prospectively enrolled. Demographics, clinical data, medications, and dietary intake were assessed. 16S rRNA gene sequencing was performed on saliva samples collected prior to endoscopy. Taxonomic differences between groups were assessed via linear discriminant analysis effect size (LEfSe). Logit models were used to develop microbiome signatures to distinguish BE from non-BE, assessed by area under the receiver operating curve (AUROC). RESULTS: A total of 49 patients were enrolled (control = 17, BE = 32). There was no significant difference in alpha diversity comparing all BE patients vs. CONTROLS: At the phylum level, the oral microbiome in BE patients had significantly increased relative abundance of Firmicutes (p = 0.005) and decreased Proteobacteria (p = 0.02). There were numerous taxonomic differences in the oral microbiome between BE and controls. A model including relative abundance of Lautropia, Streptococcus, and a genus in the order Bacteroidales distinguished BE from controls with an AUROC 0.94 (95% CI: 0.85-1.00). The optimal cutoff identified BE patients with 96.9% sensitivity and 88.2% specificity. CONCLUSIONS: The oral microbiome in BE patients was markedly altered and distinguished BE with relatively high accuracy. The oral microbiome represents a potential screening marker for BE, and validation studies in larger and distinct populations are warranted. FAU - Snider, Erik J AU - Snider EJ AD - Department of Medicine, Oregon Health Sciences University, Portland, OR, USA. FAU - Compres, Griselda AU - Compres G AD - Department of Medicine, Columbia University Medical Center, New York, NY, USA. FAU - Freedberg, Daniel E AU - Freedberg DE AD - Department of Medicine, Columbia University Medical Center, New York, NY, USA. FAU - Giddins, Marla J AU - Giddins MJ AD - Department of Medicine, Columbia University Medical Center, New York, NY, USA. AD - Microbiome & Pathogen Genomics Core, Department of Medicine, Columbia University Medical Center, New York, NY, USA. FAU - Khiabanian, Hossein AU - Khiabanian H AD - Rutgers Cancer Institute of New Jersey, Rutgers University, New Brunswick, NJ, USA. FAU - Lightdale, Charles J AU - Lightdale CJ AD - Department of Medicine, Columbia University Medical Center, New York, NY, USA. FAU - Nobel, Yael R AU - Nobel YR AD - Department of Medicine, Columbia University Medical Center, New York, NY, USA. FAU - Toussaint, Nora C AU - Toussaint NC AD - New York Genome Center, New York, NY, USA. FAU - Uhlemann, Anne-Catrin AU - Uhlemann AC AD - Department of Medicine, Columbia University Medical Center, New York, NY, USA. AD - Microbiome & Pathogen Genomics Core, Department of Medicine, Columbia University Medical Center, New York, NY, USA. FAU - Abrams, Julian A AU - Abrams JA AD - Department of Medicine, Columbia University Medical Center, New York, NY, USA. ja660@cumc.columbia.edu. LA - eng PT - Journal Article DEP - 20180220 PL - United States TA - Clin Transl Gastroenterol JT - Clinical and translational gastroenterology JID - 101532142 CIN - Clin Transl Gastroenterol. 2018 May 29;9(5):156. PMID: 29807991 PMC - PMC5862155 COIS- GUARANTORS OF THE ARTICLE: Julian Abrams. SPECIFIC AUTHORS' CONTRIBUTIONS: E.J.S.-study concept and design, study conduct, interpretation of data, manuscript preparation, critical revision of manuscript, approval of final draft submitted. G.C.-study conduct, critical revision of manuscript, approval of final draft submitted. D.E.F.-analysis and interpretation of data, critical revision of manuscript, approval of final draft submitted. M.J.G.-analysis of data, critical revision of manuscript, approval of final draft submitted. H.K.-analysis and interpretation of data, critical revision of manuscript, approval of final draft submitted. C.J.L.-study conduct, interpretation of data, critical revision of manuscript, approval of final draft submitted. Y.R.N.-interpretation of data, critical revision of manuscript, approval of final draft submitted. N.C.T.-analysis and interpretation of data, critical revision of manuscript, approval of final draft submitted. A.C.U.-analysis and interpretation of data, manuscript preparation, critical revision of manuscript, approval of final draft submitted. J.A.A.-study concept and design, study conduct, analysis and interpretation of data, manuscript preparation, critical revision of manuscript, approval of final draft submitted. FINANCIAL SUPPORT: The authors were supported in part by a Columbia Physician's and Surgeon's Dean's Research Fellowship (E.J.S.), a Career Development Award from NIDDK (K23 DK111847; D.E.F.), a U54 award from NCI (U54 CA163004; J.A.A.), a R01 from NIAID (AI116939; A.C.U.), and the Price Family Foundation. POTENTIAL COMPETING INTERESTS: None. EDAT- 2018/03/02 06:00 MHDA- 2018/03/02 06:01 PMCR- 2018/02/20 CRDT- 2018/03/02 06:00 PHST- 2017/12/08 00:00 [received] PHST- 2018/01/15 00:00 [accepted] PHST- 2018/03/02 06:00 [entrez] PHST- 2018/03/02 06:00 [pubmed] PHST- 2018/03/02 06:01 [medline] PHST- 2018/02/20 00:00 [pmc-release] AID - 10.1038/s41424-018-0005-8 [pii] AID - 5 [pii] AID - 10.1038/s41424-018-0005-8 [doi] PST - epublish SO - Clin Transl Gastroenterol. 2018 Feb 20;9(3):135. doi: 10.1038/s41424-018-0005-8.