PMID- 29495557
OWN - NLM
STAT- MEDLINE
DCOM- 20190206
LR  - 20190215
IS  - 2072-6651 (Electronic)
IS  - 2072-6651 (Linking)
VI  - 10
IP  - 3
DP  - 2018 Feb 28
TI  - Estrogen Receptor alpha Is Crucial in Zearalenone-Induced Invasion and Migration of 
      Prostate Cancer Cells.
LID - 10.3390/toxins10030098 [doi]
LID - 98
AB  - Zearalenone (ZEA), a mycotoxin produced in the genus Fusarium, binds to estrogen 
      receptors (ER) and is therefore regarded as an endocrine disruptor. ZEA has also 
      been found to modulate the proliferation and apoptosis of prostate cancer cells 
      in a dose-dependent manner. This study evaluates whether the effect of a low dose 
      of ZEA (0.1 and 0.001 nM) on the invasion and migration of prostate cancer cell 
      line PC3 is associated with ERs expression. The invasion and migration was 
      evaluated by modified Boyden chamber assay, scratch assay, gelatin zymography, 
      Real Time qPCR (RTqPCR) and Western blot. The involvement of ERs was evaluated 
      with the selective ER antagonists: estrogen receptor alpha (ERalpha) antagonist 1,3-bis 
      (4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy) phenol]-1H-pyrazole 
      dihydrochloride (MPP) and estrogen receptor beta (ERbeta) antagonist 
      4-[2-phenyl-5,7-bis (trifluoromethyl) pyrazolo [1,5-a]-pyrimidin-3-yl] phenol 
      (PHTPP). ZEA was found to modulate cell motility dependent on estrogen receptors, 
      particularly ERalpha. Increased cell migration and invasion were associated with 
      increased MMP-2 and MMP-9 activity as well as the up-regulation of the 
      EMT-associated genes vimentin (VIM), zinc finger E-box-binding homeobox 1/2 
      (ZEB1/2) and transforming growth factor beta 1 (TGFbeta1). In conclusion, ZEA might 
      modulate the invasiveness of prostate cancer cells dependently on ERalpha expression.
FAU - Kowalska, Karolina
AU  - Kowalska K
AD  - Laboratory of Cell Cultures and Genomic Analysis, Department of Comparative 
      Endocrinology, Faculty of Biomedical Sciences and Postgraduate Education, Medical 
      University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland. klakus@op.pl.
AD  - Department of Comparative Endocrinology, Faculty of Biomedical Sciences and 
      Postgraduate Education, Medical University of Lodz, Zeligowskiego 7/9, 90-752, 
      Lodz, Poland. klakus@op.pl.
FAU - Habrowska-Gorczynska, Dominika Ewa
AU  - Habrowska-Gorczynska DE
AD  - Laboratory of Cell Cultures and Genomic Analysis, Department of Comparative 
      Endocrinology, Faculty of Biomedical Sciences and Postgraduate Education, Medical 
      University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland. 
      dominika.habrowska@umed.lodz.pl.
AD  - Department of Comparative Endocrinology, Faculty of Biomedical Sciences and 
      Postgraduate Education, Medical University of Lodz, Zeligowskiego 7/9, 90-752, 
      Lodz, Poland. dominika.habrowska@umed.lodz.pl.
FAU - Urbanek, Kinga Anna
AU  - Urbanek KA
AD  - Laboratory of Cell Cultures and Genomic Analysis, Department of Comparative 
      Endocrinology, Faculty of Biomedical Sciences and Postgraduate Education, Medical 
      University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland. 
      urbanek.kinga@gmail.com.
AD  - Department of Comparative Endocrinology, Faculty of Biomedical Sciences and 
      Postgraduate Education, Medical University of Lodz, Zeligowskiego 7/9, 90-752, 
      Lodz, Poland. urbanek.kinga@gmail.com.
FAU - Dominska, Kamila
AU  - Dominska K
AD  - Department of Comparative Endocrinology, Faculty of Biomedical Sciences and 
      Postgraduate Education, Medical University of Lodz, Zeligowskiego 7/9, 90-752, 
      Lodz, Poland. kamila.dominska@umed.lodz.pl.
FAU - Piastowska-Ciesielska, Agnieszka Wanda
AU  - Piastowska-Ciesielska AW
AUID- ORCID: 0000-0001-7462-8528
AD  - Laboratory of Cell Cultures and Genomic Analysis, Department of Comparative 
      Endocrinology, Faculty of Biomedical Sciences and Postgraduate Education, Medical 
      University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland. 
      agnieszka.piastowska@umed.lodz.pl.
AD  - Department of Comparative Endocrinology, Faculty of Biomedical Sciences and 
      Postgraduate Education, Medical University of Lodz, Zeligowskiego 7/9, 90-752, 
      Lodz, Poland. agnieszka.piastowska@umed.lodz.pl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180228
PL  - Switzerland
TA  - Toxins (Basel)
JT  - Toxins
JID - 101530765
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Estrogen Receptor alpha)
RN  - 5W827M159J (Zearalenone)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Endocrine Disruptors/*pharmacology
MH  - Estrogen Receptor alpha/*metabolism
MH  - Humans
MH  - Male
MH  - Neoplasm Invasiveness
MH  - Prostatic Neoplasms/*metabolism/*pathology
MH  - Zearalenone/*pharmacology
PMC - PMC5869386
OTO - NOTNLM
OT  - estrogen receptors
OT  - mycotoxins
OT  - zearalenone
COIS- The authors declare no conflict of interest.
EDAT- 2018/03/03 06:00
MHDA- 2019/02/07 06:00
PMCR- 2018/03/01
CRDT- 2018/03/03 06:00
PHST- 2017/12/12 00:00 [received]
PHST- 2018/01/28 00:00 [revised]
PHST- 2018/02/22 00:00 [accepted]
PHST- 2018/03/03 06:00 [entrez]
PHST- 2018/03/03 06:00 [pubmed]
PHST- 2019/02/07 06:00 [medline]
PHST- 2018/03/01 00:00 [pmc-release]
AID - toxins10030098 [pii]
AID - toxins-10-00098 [pii]
AID - 10.3390/toxins10030098 [doi]
PST - epublish
SO  - Toxins (Basel). 2018 Feb 28;10(3):98. doi: 10.3390/toxins10030098.