PMID- 29496310 OWN - NLM STAT- MEDLINE DCOM- 20181015 LR - 20181015 IS - 1618-0631 (Electronic) IS - 0344-0338 (Linking) VI - 214 IP - 4 DP - 2018 Apr TI - Wnt/beta-catenin signal alteration and its diagnostic utility in basal cell adenoma and histologically similar tumors of the salivary gland. PG - 586-592 LID - S0344-0338(17)31131-7 [pii] LID - 10.1016/j.prp.2017.12.016 [doi] AB - Differential diagnosis among basal cell adenoma (BCA), basal cell adenocarcinoma (BCAC), adenoid cystic carcinoma (ACC) and pleomorphic adenoma (PA) of the salivary gland can be challenging due to their similar histological appearance. Although frequent nuclear beta-catenin expression and CTNNB1 mutations have been reported in BCA, further details of the Wnt/beta-catenin signal alterations are unclear. The aim of this study was to assess the diagnostic utility of Wnt/beta-catenin signal alteration in BCA and morphological mimics. We performed immunohistochemical staining for beta-catenin and mutation analysis for Wnt/beta-catenin-related genes (CTNNB1, APC, AXIN1 and AXIN2) in BCA (n = 34), BCAC (n = 3), ACC (n = 67) and PA (n = 31). We also analyzed ACC-specific MYB and MYBL1 gene rearrangements by fluorescence in situ hybridization (FISH). Nuclear beta-catenin expression (>/=3%) was present in 32/34 cases (94.1%) of BCA, and the nuclear beta-catenin labeling index was significantly higher than in other tumor types (p = < 0.0001). In BCA, we found mutations in CTNNB1, APC and AXIN1 genes (41.1%, 2.9% and 8.8%, respectively). In BCAC, nuclear beta-catenin expression with CTNNB1 mutation was present in 1/3 cases (33.3%). As for ACC, nuclear beta-catenin expression was observed in 3/67 cases (4.4%), but all 3 cases harbored either MYB or MYBL1 gene rearrangement. The results suggest that nuclear beta-catenin immunoreactivity with appropriate criteria may be helpful to distinguish BCA from histologically similar tumors. However, a minor subset of ACCs with nuclear beta-catenin expression require careful diagnosis. In addition, Wnt/beta-catenin signal alteration may play a role in the pathogenesis of BCA and BCAC. CI - Copyright (c) 2018 Elsevier GmbH. All rights reserved. FAU - Sato, Masanobu AU - Sato M AD - Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka, 812-8582, Japan. FAU - Yamamoto, Hidetaka AU - Yamamoto H AD - Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka, 812-8582, Japan. FAU - Hatanaka, Yui AU - Hatanaka Y AD - Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka, 812-8582, Japan. FAU - Nishijima, Toshimitsu AU - Nishijima T AD - Department of Otorhinolaryngology, Japan Community Health Care Organization Kyushu Hospital, 1-8-1 Kishinoura, Yahatanishi-ku, Kitakyushu-shi, Fukuoka, 806-8501, Japan. FAU - Jiromaru, Rina AU - Jiromaru R AD - Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka, 812-8582, Japan. FAU - Yasumatsu, Ryuji AU - Yasumatsu R AD - Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka, 812-8582, Japan. FAU - Taguchi, Kenichi AU - Taguchi K AD - Department of Pathology, National Kyushu Cancer Center, 3-1-1 Notame, Minami-ku, Fukuoka-shi, Fukuoka, 811-1395, Japan. FAU - Masuda, Muneyuki AU - Masuda M AD - Department of Head and Neck Surgery, National Kyushu Cancer Center, 3-1-1 Notame, Minami-ku, Fukuoka-shi, Fukuoka, 811-1395, Japan. FAU - Nakagawa, Takashi AU - Nakagawa T AD - Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka, 812-8582, Japan. FAU - Oda, Yoshinao AU - Oda Y AD - Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka, 812-8582, Japan. Electronic address: oda@surgpath.med.kyushu-u.ac.jp. LA - eng PT - Journal Article DEP - 20180103 PL - Germany TA - Pathol Res Pract JT - Pathology, research and practice JID - 7806109 RN - 0 (APC protein, human) RN - 0 (AXIN2 protein, human) RN - 0 (Adenomatous Polyposis Coli Protein) RN - 0 (Axin Protein) RN - 0 (Biomarkers, Tumor) RN - 0 (CTNNB1 protein, human) RN - 0 (beta Catenin) SB - IM MH - Adenocarcinoma/*diagnosis/genetics/metabolism/pathology MH - Adenoma/*diagnosis/genetics/metabolism/pathology MH - Adenoma, Pleomorphic/*diagnosis/genetics/metabolism/pathology MH - Adenomatous Polyposis Coli Protein/genetics/metabolism MH - Adolescent MH - Adult MH - Aged MH - Aged, 80 and over MH - Axin Protein/genetics/metabolism MH - Biomarkers, Tumor/genetics/metabolism MH - Carcinoma, Adenoid Cystic/*diagnosis/genetics/metabolism/pathology MH - Female MH - Humans MH - Male MH - Middle Aged MH - Mutation MH - Salivary Gland Neoplasms/*diagnosis/genetics/metabolism/pathology MH - Salivary Glands/metabolism/pathology MH - *Wnt Signaling Pathway MH - Young Adult MH - beta Catenin/genetics/metabolism OTO - NOTNLM OT - APC OT - AXIN OT - Adenoid cystic carcinoma OT - Basal cell adenoma OT - Salivary gland OT - beta-catenin EDAT- 2018/03/03 06:00 MHDA- 2018/10/16 06:00 CRDT- 2018/03/03 06:00 PHST- 2017/11/09 00:00 [received] PHST- 2017/12/07 00:00 [revised] PHST- 2017/12/31 00:00 [accepted] PHST- 2018/03/03 06:00 [pubmed] PHST- 2018/10/16 06:00 [medline] PHST- 2018/03/03 06:00 [entrez] AID - S0344-0338(17)31131-7 [pii] AID - 10.1016/j.prp.2017.12.016 [doi] PST - ppublish SO - Pathol Res Pract. 2018 Apr;214(4):586-592. doi: 10.1016/j.prp.2017.12.016. Epub 2018 Jan 3.