PMID- 29498307
OWN - NLM
STAT- MEDLINE
DCOM- 20181217
LR  - 20211204
IS  - 1543-5180 (Electronic)
IS  - 1543-5180 (Linking)
VI  - 24
IP  - 1
DP  - 2018 Dec
TI  - Kruppel-like factor 4 mediates cellular migration and invasion by altering RhoA 
      activity.
PG  - 1-10
LID - 10.1080/15419061.2018.1444034 [doi]
AB  - Kru ppel like factor 4 (KLF4) is a transcription factor that regulates genes 
      related to differentiation and proliferation. KLF4 also plays a role in 
      metastasis via epithelial to mesenchymal transition. Here, we investigate the 
      function of Klf4 in migration and invasion using mouse embryonic fibroblasts and 
      the RKO human colon cancer cell line. Compared to wild-type, cells lacking Klf4 
      exhibited increased migration-associated phenotypes. In addition, overexpression 
      of Klf4 in Klf4(-/-) MEFs attenuated the presence of stress fibers to wild-type 
      levels. An invasion assay suggested that lack of Klf4 resulted in increased 
      invasive capacity. Finally, analysis of RhoA showed elevated RhoA activity in 
      both RKO and MEF cells. Taken together, our results strongly support the novel 
      role of KLF4 in a post-translational regulatory mechanism where KLF4 indirectly 
      modulates the actin cytoskeleton morphology via activity of RhoA in order to 
      inhibit cellular migration and invasion.
FAU - Brauer, Philip R
AU  - Brauer PR
AD  - a Department of Biology , Colgate University , Hamilton , NY , USA.
FAU - Kim, Jee Hun
AU  - Kim JH
AD  - a Department of Biology , Colgate University , Hamilton , NY , USA.
FAU - Ochoa, Humberto J
AU  - Ochoa HJ
AD  - a Department of Biology , Colgate University , Hamilton , NY , USA.
AD  - b Center for Cancer Research, Lab of Cancer Biology and Genetics , National 
      Cancer Institute , Bethesda , MD , USA.
FAU - Stratton, Elizabeth R
AU  - Stratton ER
AD  - a Department of Biology , Colgate University , Hamilton , NY , USA.
FAU - Black, Kathryn M
AU  - Black KM
AD  - a Department of Biology , Colgate University , Hamilton , NY , USA.
AD  - c School of Medicine , Tulane University , New Orleans , LA , USA.
FAU - Rosencrans, William
AU  - Rosencrans W
AD  - a Department of Biology , Colgate University , Hamilton , NY , USA.
FAU - Stacey, Eliza
AU  - Stacey E
AD  - a Department of Biology , Colgate University , Hamilton , NY , USA.
FAU - Hagos, Engda G
AU  - Hagos EG
AD  - a Department of Biology , Colgate University , Hamilton , NY , USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Commun Adhes
JT  - Cell communication & adhesion
JID - 101096596
RN  - 0 (KLF4 protein, human)
RN  - 0 (Klf4 protein, mouse)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.5.2 (Rhoc protein, mouse)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
RN  - EC 3.6.5.2 (rhoC GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - *Cell Movement
MH  - Cell Shape
MH  - Embryo, Mammalian/cytology
MH  - Fibroblasts/*cytology/*metabolism
MH  - Guanosine Triphosphate/metabolism
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors/deficiency/*metabolism
MH  - Mice, Inbred C57BL
MH  - Stress Fibers/metabolism
MH  - Up-Regulation/genetics
MH  - rhoA GTP-Binding Protein/genetics/*metabolism
MH  - rhoC GTP-Binding Protein/genetics/metabolism
OTO - NOTNLM
OT  - G protein
OT  - Kruppel-like factor 4
OT  - RhoA
OT  - actin cytoskeleton
OT  - invasion
OT  - migration
EDAT- 2018/03/03 06:00
MHDA- 2018/12/18 06:00
CRDT- 2018/03/03 06:00
PHST- 2018/03/03 06:00 [entrez]
PHST- 2018/03/03 06:00 [pubmed]
PHST- 2018/12/18 06:00 [medline]
AID - 10.1080/15419061.2018.1444034 [doi]
PST - ppublish
SO  - Cell Commun Adhes. 2018 Dec;24(1):1-10. doi: 10.1080/15419061.2018.1444034.