PMID- 29499096
OWN - NLM
STAT- MEDLINE
DCOM- 20191108
LR  - 20191108
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 12
IP  - 5
DP  - 2018 May
TI  - Functional collagen conduits combined with human mesenchymal stem cells promote 
      regeneration after sciatic nerve transection in dogs.
PG  - 1285-1296
LID - 10.1002/term.2660 [doi]
AB  - Numerous studies have focused on the development of novel and innovative 
      approaches for the treatment of peripheral nerve injury using artificial nerve 
      guide conduits. In this study, we attempted to bridge 3.5-cm defects of the 
      sciatic nerve with a longitudinally oriented collagen conduit (LOCC) loaded with 
      human umbilical cord mesenchymal stem cells (hUC-MSCs). The LOCC contains a 
      bundle of longitudinally aligned collagenous fibres enclosed in a hollow collagen 
      tube. Our previous studies showed that an LOCC combined with neurotrophic factors 
      enhances peripheral nerve regeneration. However, it remained unknown whether an 
      LOCC seeded with hUC-MSCs could also promote regeneration. In this study, using 
      various histological and electrophysiological analyses, we found that an LOCC 
      provides mechanical support to newly growing nerves and functions as a structural 
      scaffold for cells, thereby stimulating sciatic nerve regeneration. The LOCC and 
      hUC-MSCs synergistically promoted regeneration and improved the functional 
      recovery in a dog model of sciatic nerve injury. Therefore, the combined use of 
      an LOCC and hUC-MSCs might have therapeutic potential for the treatment of 
      peripheral nerve injury.
CI  - Copyright (c) 2018 John Wiley & Sons, Ltd.
FAU - Cui, Yi
AU  - Cui Y
AD  - Reproductive and Genetic Center of National Research Institute for Family 
      Planning, Beijing, China.
AD  - Key Laboratory of Molecular Developmental Biology, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Beijing, China.
FAU - Yao, Yao
AU  - Yao Y
AD  - Department of Prosthodontics, School of Stomatology, Capital Medical University, 
      Beijing, China.
FAU - Zhao, Yannan
AU  - Zhao Y
AD  - Key Laboratory of Molecular Developmental Biology, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Beijing, China.
FAU - Xiao, Zhifeng
AU  - Xiao Z
AD  - Key Laboratory of Molecular Developmental Biology, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Beijing, China.
FAU - Cao, Zongfu
AU  - Cao Z
AD  - Reproductive and Genetic Center of National Research Institute for Family 
      Planning, Beijing, China.
FAU - Han, Sufang
AU  - Han S
AD  - Key Laboratory of Molecular Developmental Biology, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Beijing, China.
FAU - Li, Xing
AU  - Li X
AD  - Key Laboratory of Molecular Developmental Biology, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Beijing, China.
FAU - Huan, Yong
AU  - Huan Y
AD  - State Key Laboratory of Nonlinear Mechanics (LNM), Institute of Mechanics, 
      Chinese Academy of Sciences, Beijing, China.
FAU - Pan, Juli
AU  - Pan J
AD  - Department of Prosthodontics, School of Stomatology, Capital Medical University, 
      Beijing, China.
FAU - Dai, Jianwu
AU  - Dai J
AUID- ORCID: 0000-0002-3379-9053
AD  - Key Laboratory of Molecular Developmental Biology, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180325
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Collagen/*pharmacology
MH  - Dogs
MH  - Electrophysiological Phenomena/drug effects
MH  - Humans
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology/ultrastructure
MH  - Muscles/drug effects
MH  - *Nerve Regeneration
MH  - Sciatic Nerve/drug effects/*injuries/*physiopathology/ultrastructure
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - dog
OT  - human umbilical cord mesenchymal stem cells (hUC-MSCs)
OT  - longitudinally oriented collagen conduit (LOCC)
OT  - nerve regeneration
OT  - sciatic nerve
EDAT- 2018/03/03 06:00
MHDA- 2019/11/09 06:00
CRDT- 2018/03/03 06:00
PHST- 2017/03/21 00:00 [received]
PHST- 2018/01/08 00:00 [revised]
PHST- 2018/02/17 00:00 [accepted]
PHST- 2018/03/03 06:00 [pubmed]
PHST- 2019/11/09 06:00 [medline]
PHST- 2018/03/03 06:00 [entrez]
AID - 10.1002/term.2660 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2018 May;12(5):1285-1296. doi: 10.1002/term.2660. Epub 
      2018 Mar 25.