PMID- 29500045 OWN - NLM STAT- MEDLINE DCOM- 20201015 LR - 20201015 IS - 1995-9133 (Electronic) IS - 1684-1182 (Linking) VI - 53 IP - 1 DP - 2020 Feb TI - Human papillomavirus infection is not associated with laryngeal squamous cell carcinoma in Taiwan. PG - 79-86 LID - S1684-1182(18)30047-1 [pii] LID - 10.1016/j.jmii.2018.02.002 [doi] AB - BACKGROUND/PURPOSE: To examine whether the prevalence rate of human papillomavirus (HPV) infection in Taiwanese patients with primary laryngeal squamous cell carcinoma (LSCC) is different from that in those with a vocal polyp (VP) or vocal fold leukoplakia (VFL). METHODS: This prospective cohort study recruited 41 consecutive patients with primary LSCC and 27 and 20 patients with VP and VFL, respectively. The HPV L1 gene in surgical specimens was detected using polymerase chain reaction. High-risk HPV DNA in tissue microarray specimens was detected using in situ hybridization. Expression of p16(INK4a) in tissue microarray specimens was determined through immunohistochemistry. RESULTS: The prevalence of HPV L1 DNA in the LSCC group was equivalent to that in the VP and VFL groups (7.3% vs. 7.4% vs. 10.0%; P = 0.929; effect size = 0.20). High-risk HPV DNA detected using in situ hybridization was relatively rare in all groups (2.6% vs. 5.3% vs. 0.0%; P = 0.636; effect size = 0.81). The prevalence of p16(INK4a) positivity was significantly lower in the LSCC group than in the VP and VFL groups (5.1% vs. 58.8% vs. 14.3%; P < 0.001). Multivariate analysis results revealed that age >/=65 years (adjusted odds ratio, 4.09; 95% confidence interval, 1.21-13.91; P = 0.024) and p16(INK4a) positivity (adjusted odds ratio, 0.10; 95% confidence interval, 0.02-0.53; P = 0.006) were LSCC risk factors. CONCLUSION: HPV infection is uncommon in Taiwanese patients with LSCC and seems not to be associated with an increased LSCC risk. Larger sample size is warranted for further study. CI - Copyright (c) 2018. Published by Elsevier B.V. FAU - Huang, Chung-Guei AU - Huang CG AD - Graduate Institute of Biomedical Sciences, Chang Gung University, Taoyuan, Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. FAU - Lee, Li-Ang AU - Lee LA AD - Department of Otorhinolaryngology - Head and Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Faculty of Medicine, Chang Gung University, Taoyuan, Taiwan. Electronic address: 5738@cgmh.org.tw. FAU - Fang, Tuan-Jen AU - Fang TJ AD - Department of Otorhinolaryngology - Head and Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Faculty of Medicine, Chang Gung University, Taoyuan, Taiwan. FAU - Li, Hsueh-Yu AU - Li HY AD - Department of Otorhinolaryngology - Head and Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Faculty of Medicine, Chang Gung University, Taoyuan, Taiwan. FAU - Liao, Chun-Ta AU - Liao CT AD - Department of Otorhinolaryngology - Head and Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Faculty of Medicine, Chang Gung University, Taoyuan, Taiwan. FAU - Kang, Chung-Jan AU - Kang CJ AD - Department of Otorhinolaryngology - Head and Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Faculty of Medicine, Chang Gung University, Taoyuan, Taiwan. FAU - Yen, Tzu-Chen AU - Yen TC AD - Department of Otorhinolaryngology - Head and Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Department of Nuclear Medicine and Molecular Imaging Center, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. FAU - Tsao, Kuo-Chien AU - Tsao KC AD - Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. FAU - Chen, Tse-Ching AU - Chen TC AD - Department of Otorhinolaryngology - Head and Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Department of Pathology, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. FAU - Shih, Shin-Ru AU - Shih SR AD - Graduate Institute of Biomedical Sciences, Chang Gung University, Taoyuan, Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. Electronic address: srshih@mail.cgu.edu.tw. LA - eng PT - Journal Article DEP - 20180221 PL - England TA - J Microbiol Immunol Infect JT - Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi JID - 100956211 RN - 0 (Capsid Proteins) RN - 0 (DNA, Viral) RN - 0 (HPV L1 protein, Human papillomavirus) RN - 0 (Oncogene Proteins, Viral) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Capsid Proteins/genetics MH - Carcinoma, Squamous Cell/*epidemiology MH - DNA, Viral/genetics MH - Female MH - Humans MH - Immunohistochemistry MH - Laryngeal Neoplasms/*epidemiology MH - Male MH - Middle Aged MH - Oncogene Proteins, Viral/genetics MH - Papillomavirus Infections/*epidemiology MH - Prognosis MH - Prospective Studies MH - Risk Factors MH - Taiwan/epidemiology MH - Tissue Array Analysis OTO - NOTNLM OT - Human papillomavirus OT - Laryngeal squamous cell carcinoma OT - Prevalence OT - Prospective cohort study OT - Risk factor EDAT- 2018/03/04 06:00 MHDA- 2020/10/21 06:00 CRDT- 2018/03/04 06:00 PHST- 2017/09/04 00:00 [received] PHST- 2017/11/27 00:00 [revised] PHST- 2018/02/05 00:00 [accepted] PHST- 2018/03/04 06:00 [pubmed] PHST- 2020/10/21 06:00 [medline] PHST- 2018/03/04 06:00 [entrez] AID - S1684-1182(18)30047-1 [pii] AID - 10.1016/j.jmii.2018.02.002 [doi] PST - ppublish SO - J Microbiol Immunol Infect. 2020 Feb;53(1):79-86. doi: 10.1016/j.jmii.2018.02.002. Epub 2018 Feb 21.