PMID- 29500491
OWN - NLM
STAT- MEDLINE
DCOM- 20190708
LR  - 20190708
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Linking)
VI  - 373
IP  - 2
DP  - 2018 Aug
TI  - Overexpression of FOXQ1 enhances anti-senescence and migration effects of human 
      umbilical cord mesenchymal stem cells in vitro and in vivo.
PG  - 379-393
LID - 10.1007/s00441-018-2815-0 [doi]
AB  - Mesenchymal stem cells (MSCs) are unique precursor cells characterized by active 
      self-renewal and differentiation potential. These cells offer the advantages of 
      ease of isolation and limited ethical issues as a resource and represent a 
      promising cell therapy for neurodegenerative diseases. However, replicative 
      senescence during cell culture as well as low efficiency of cell migration and 
      differentiation after transplantation are major obstacles. In our previous study, 
      we found that FOXQ1 binds directly to the SIRT1 promoter to regulate cellular 
      senescence and also promotes cell proliferation and migration in many tumor cell 
      lines. Currently, little is known about the effects of FOXQ1 on normal somatic 
      cells. Therefore, we examine the effects of FOXQ1 on senescence and migration of 
      MSCs. Lentiviral vector-mediated overexpression of FOXQ1 in human umbilical cord 
      mesenchymal stem cells (hUC-MSCs) resulted in enhanced cell proliferation and 
      viability. Furthermore, the expression of proteins and markers positively 
      associated with senescence (p16, p21, p53) was reduced, whereas expression of 
      proteins negatively associated with senescence (SIRT1, PCNA) was promoted. 
      Following transplantation of hUC-MSCs overexpressing FOXQ1 in an animal model of 
      Alzheimer's disease (APPV717I transgenic mice) resulted in amelioration of the 
      effects of Alzheimer's disease (AD) on cognitive function and pathological 
      senescence accompanied the increased numbers of hUC-MSCs in the AD brain. In 
      conclusion, FOXQ1 overexpression promotes anti-senescence and migration of 
      hUC-MSCs in vitro and in vivo. These findings also suggest that this strategy may 
      contribute to optimization of the efficiency of stem cell therapy.
FAU - Zhang, Tao
AU  - Zhang T
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China.
FAU - Wang, Pan
AU  - Wang P
AD  - The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, 
      China.
FAU - Liu, Yanxia
AU  - Liu Y
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China.
FAU - Zhou, Jiankang
AU  - Zhou J
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China.
FAU - Shi, Zhenqing
AU  - Shi Z
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China.
FAU - Cheng, Kang
AU  - Cheng K
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China.
FAU - Huang, Tuanjie
AU  - Huang T
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China.
FAU - Wang, Xinxin
AU  - Wang X
AD  - The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, 
      China.
FAU - Yang, Greta Luyuan
AU  - Yang GL
AD  - Stuyvesant High School, New York, NY, 10282, USA.
FAU - Yang, Bo
AU  - Yang B
AD  - The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, 
      China.
FAU - Ma, Shanshan
AU  - Ma S
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China. 
      mashanshan84@163.com.
FAU - Guan, Fangxia
AU  - Guan F
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China. 
      guanfangxia@126.com.
AD  - The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, 
      China. guanfangxia@126.com.
LA  - eng
GR  - 17A310012/The Key Research Project of Higher Education of Henan Province/
GR  - 154200510008/The Plan for Scientific Innovation Talent of Henan Province/
GR  - 2016GH15/The International Collaboration Research Program for Talents of Henan 
      Province/
GR  - 20114101110004/Research Fund for the Doctoral Program of Higher Education of 
      China/
GR  - 15IRTSTHN022/Innovative Research Team in Science and Technology of the University 
      of Henan Province/
GR  - 2015M572122/China Postdoctoral Science Foundation/
GR  - 81501200/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20180303
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (FOXQ1 protein, human)
RN  - 0 (Forkhead Transcription Factors)
SB  - IM
MH  - Alzheimer Disease/metabolism/pathology
MH  - Animals
MH  - Cell Differentiation/genetics
MH  - Cell Movement/*genetics
MH  - Cell Proliferation/genetics
MH  - Cellular Senescence/*genetics
MH  - Disease Models, Animal
MH  - Forkhead Transcription Factors/genetics/*metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Mice, Transgenic
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - Anti-senescence
OT  - FOXQ1
OT  - Human umbilical cord mesenchymal stem cells
OT  - Migration
OT  - Overexpression
EDAT- 2018/03/04 06:00
MHDA- 2019/07/10 06:00
CRDT- 2018/03/04 06:00
PHST- 2017/08/13 00:00 [received]
PHST- 2018/01/30 00:00 [accepted]
PHST- 2018/03/04 06:00 [pubmed]
PHST- 2019/07/10 06:00 [medline]
PHST- 2018/03/04 06:00 [entrez]
AID - 10.1007/s00441-018-2815-0 [pii]
AID - 10.1007/s00441-018-2815-0 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2018 Aug;373(2):379-393. doi: 10.1007/s00441-018-2815-0. Epub 
      2018 Mar 3.