PMID- 29501009
OWN - NLM
STAT- MEDLINE
DCOM- 20180910
LR  - 20180910
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 57
DP  - 2018 Apr
TI  - In vitro maturation of monocyte-derived dendritic cells results in two 
      populations of cells with different surface marker expression, independently of 
      applied concentration of interleukin-4.
PG  - 165-171
LID - S1567-5769(18)30085-7 [pii]
LID - 10.1016/j.intimp.2018.02.015 [doi]
AB  - Dendritic cells (DCs) play a crucial role in the development of adaptive immune 
      response. Monocyte-derived dendritic cells (MDDCs) are generated in vitro to 
      study DC biology and for use in immunotherapy. However, procedures to generate 
      MDDCs vary and an impact this may have on their final phenotype is insufficiently 
      studied. Monocytes isolated from healthy blood donors were cultured for 7 days 
      with granulocyte-macrophage colony stimulating factor (50 ng/mL) and low 
      (500 IU/mL, L-IL4) or high (1000 IU/mL, H-IL4) interleukin 4 (IL4), to obtain 
      immature DCs and for the following 2 days with addition of soluble CD40 ligand 
      (500 ng/mL) and prostaglandin E(2) (1 mug/mL) to obtain mature DCs. We measured 
      mean fluorescence activity and percentage of cells, positive for CD14, HLA-DR, 
      CD80, CD83, CD86, CCR7, and CD1a or CD209 markers after 7 and 9 days of culture, 
      in both IL4 concentrations. Percentage of positively staining mature MDDCs was 
      higher than among immature cells, for all studied markers. Interestingly, varying 
      IL4 concentrations had negligible impact on staining of mature MDDCs. However, 
      immature L-IL4 cultured MDDCs were less intensely stained for HLA-DR and CD209 
      than H-IL4 immature DCs. Flow cytometry revealed presence of 2 populations of 
      cells (dominant P1 and less prevalent P2), when either L-IL4 or H-IL4 was used. 
      Among mature MDDCs, population P1 had higher percentage of positively staining 
      cells than P2, for all studied markers except CCR7. In conclusion, both 
      concentrations of IL4 produce in vitro heterogeneous populations of mature MDDCs 
      with similar staining for cell surface markers.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Wolkow, Pawel P
AU  - Wolkow PP
AD  - Jagiellonian University Medical College, Faculty of Medicine, Department of 
      Pharmacology, Ul. Grzegorzecka 16, 31-531 Krakow, Poland. Electronic address: 
      Pawel.Wolkow@uj.edu.pl.
FAU - Gebska, Anna
AU  - Gebska A
AD  - Jagiellonian University Medical College, Faculty of Medicine, Department of 
      Pharmacology, Ul. Grzegorzecka 16, 31-531 Krakow, Poland.
FAU - Korbut, Ryszard
AU  - Korbut R
AD  - Jagiellonian University Medical College, Faculty of Medicine, Department of 
      Pharmacology, Ul. Grzegorzecka 16, 31-531 Krakow, Poland.
LA  - eng
PT  - Journal Article
DEP - 20180228
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Biomarkers)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Cell Differentiation
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Dendritic Cells/*physiology
MH  - Dinoprostone/metabolism
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Humans
MH  - Immunotherapy/*methods
MH  - Interleukin-4/*metabolism
MH  - Monocytes/physiology
OTO - NOTNLM
OT  - Dendritic cell maturation
OT  - Flow cytometry
OT  - Interleukin 4 (IL4)
OT  - Monocyte-derived dendritic cells (MDDCs)
EDAT- 2018/03/04 06:00
MHDA- 2018/09/11 06:00
CRDT- 2018/03/04 06:00
PHST- 2017/09/19 00:00 [received]
PHST- 2018/02/16 00:00 [revised]
PHST- 2018/02/17 00:00 [accepted]
PHST- 2018/03/04 06:00 [pubmed]
PHST- 2018/09/11 06:00 [medline]
PHST- 2018/03/04 06:00 [entrez]
AID - S1567-5769(18)30085-7 [pii]
AID - 10.1016/j.intimp.2018.02.015 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2018 Apr;57:165-171. doi: 10.1016/j.intimp.2018.02.015. Epub 
      2018 Feb 28.