PMID- 29501195
OWN - NLM
STAT- MEDLINE
DCOM- 20180907
LR  - 20180907
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 182
DP  - 2018 May 15
TI  - SUPRAS extraction approach for matrix-independent determination of 
      amphetamine-type stimulants by LC-MS/MS.
PG  - 574-582
LID - S0039-9140(18)30148-6 [pii]
LID - 10.1016/j.talanta.2018.02.039 [doi]
AB  - Monitoring of amphetamine-type stimulant (ATS) confronts clinical labs with a 
      high number of samples involving a variety of biological matrices. Liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS), routinely used for 
      confirmation of ATS abuse, requires of laborious and matrix-dependent sample 
      treatment methods, this increasing analysis time and cost. In this work, a 
      universal and single-step sample treatment, based on supramolecular solvents 
      (SUPRAS), was proposed for simplifying ATS confirmation in seven biological 
      matrices. The SUPRAS was synthesized in situ in the sample (900 microL of basified 
      oral fluid, urine, serum, sweat or breast milk or 50 mg of digested hair or 
      fingernails) by the addition of hexanol (200 microL) and tetrahydrofuran (900 microL). 
      The mixture was vortex-shaken and centrifuged and the SUPRAS extract was 
      subsequently analyzed by positive ion mode electrospray LC-MS/MS. The method was 
      fully validated for amphetamine (AMP), methamphetamine (MA), 
      3,4-methylenedioxyamphetamine (MDA), N-ethyl-3,4-methylenedioxyamphetamine (MDEA) 
      and N-methyl-3,4-methylenedioxyamphetamine (MDMA). Maximum ion suppression or 
      enhancement was 9% and 7%, respectively, and extraction recoveries (87-111%) and 
      within- (0.1-6.7%) and between-day (0.3-9.7%) CVs were all within required 
      values. The lower limits of quantification (LLOQ) for biological fluids 
      (5 ng/mL), and hair and fingernails (100 ng/g) were all well below the cut-offs 
      established by worldwide organizations. Confirmation of MDA was carried out in 
      five urine samples that tested positive for ATS by immunoassay. The 
      SUPRAS-LC-MS/MS methodology succeeded in developing a hitherto unexplored and 
      universal tool for quantifying ATS in a comprehensive pool of biological matrices 
      of interest in forensic and clinical samples.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Accioni, Francesca
AU  - Accioni F
AD  - Department of Analytical Chemistry, Institute of Fine Chemistry and 
      Nanochemistry, Universidad de Cordoba, Spain; Department of Chemistry and 
      Pharmacy, University of Sassari, Italy.
FAU - Garcia-Gomez, Diego
AU  - Garcia-Gomez D
AD  - Department of Analytical Chemistry, Institute of Fine Chemistry and 
      Nanochemistry, Universidad de Cordoba, Spain. Electronic address: 
      dgarcia1@uco.es.
FAU - Girela, Eloy
AU  - Girela E
AD  - Department of Pharmacology, Toxicology and Legal and Forensic Medicine, 
      Universidad de Cordoba, Spain.
FAU - Rubio, Soledad
AU  - Rubio S
AD  - Department of Analytical Chemistry, Institute of Fine Chemistry and 
      Nanochemistry, Universidad de Cordoba, Spain.
LA  - eng
PT  - Journal Article
DEP - 20180209
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Amphetamines)
RN  - 0 (Central Nervous System Stimulants)
RN  - 0 (Furans)
RN  - 0 (Hexanols)
RN  - 3N8FZZ6PY4 (tetrahydrofuran)
SB  - IM
MH  - Amphetamines/blood/classification/*urine
MH  - Central Nervous System Stimulants/blood/classification/*urine
MH  - Chromatography, Liquid
MH  - Furans/*chemistry
MH  - Hair/chemistry
MH  - Hexanols/*chemistry
MH  - Humans
MH  - Hydrolysis
MH  - Limit of Detection
MH  - Liquid-Liquid Extraction/*methods
MH  - Milk, Human/chemistry
MH  - Nails/chemistry
MH  - Saliva/chemistry
MH  - Substance Abuse Detection/*methods
MH  - Sweat/chemistry
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Amphetamines
OT  - Biological samples
OT  - Liquid chromatography
OT  - Sample treatment
OT  - Supramolecular solvents
OT  - Tandem mass spectrometry
EDAT- 2018/03/05 06:00
MHDA- 2018/09/08 06:00
CRDT- 2018/03/05 06:00
PHST- 2017/12/12 00:00 [received]
PHST- 2018/02/06 00:00 [revised]
PHST- 2018/02/08 00:00 [accepted]
PHST- 2018/03/05 06:00 [entrez]
PHST- 2018/03/05 06:00 [pubmed]
PHST- 2018/09/08 06:00 [medline]
AID - S0039-9140(18)30148-6 [pii]
AID - 10.1016/j.talanta.2018.02.039 [doi]
PST - ppublish
SO  - Talanta. 2018 May 15;182:574-582. doi: 10.1016/j.talanta.2018.02.039. Epub 2018 
      Feb 9.