PMID- 29502225
OWN - NLM
STAT- MEDLINE
DCOM- 20191219
LR  - 20210109
IS  - 1532-2807 (Electronic)
IS  - 1219-4956 (Linking)
VI  - 25
IP  - 3
DP  - 2019 Jul
TI  - Quantitative Proteomics Identify the Possible Tumor Suppressive Role of 
      Protease-Activated Receptor-4 in Esophageal Squamous Cell Carcinoma Cells.
PG  - 937-943
LID - 10.1007/s12253-018-0395-7 [doi]
AB  - Exposure to carcinogens of tobacco smoke may result in methylation of 
      protease-activated receptors-4 (PAR4) gene and further induces the loss of PAR4 
      expression, which is considered to be involved in carcinogenesis of esophageal 
      squamous cell carcinoma (ESCC). Here we employed a TMT-based quantitative 
      proteomic approach to identify PAR4-regulated changes of proteomic profiles in 
      ESCC cells and to identify potentially therapeutic value. A total of 33 proteins 
      were found significantly changed with 15 up-regulated and 18 down-regulated in 
      PAR4-activating peptide (PAR4-AP) treated ESCC cells compared with controls. 
      Bioinformatics analysis showed that key higher expressed proteins included those 
      associated with apoptosis and tumor suppressor (e.g. CASP9), and lower expressed 
      proteins included those associated with anti-apoptosis, autophagy and promoting 
      cell proliferation (e.g. CHMP1B, PURA, PARG and HIST1H2AH). Western blot verified 
      changes in five representative proteins including CASP9, CHMP1B, PURA, PARG and 
      HIST1H2AH. Immunohistochemistry analysis showed that CHMP1B, PURA, PARG and 
      HIST1H2AH expression in ESCC tissues were significantly higher than those in 
      adjacent nontumorous tissues. Our findings will be helpful in further 
      investigations into the functions and molecular mechanisms of PAR4 in ESCC.
FAU - Wang, Ming
AU  - Wang M
AD  - Department of Human Anatomy, Taishan Medical University, 2 Ying Sheng Dong Lu, 
      Tai'an, 271000, China.
FAU - An, Shuhong
AU  - An S
AD  - Department of Human Anatomy, Taishan Medical University, 2 Ying Sheng Dong Lu, 
      Tai'an, 271000, China.
FAU - Wang, Diyi
AU  - Wang D
AD  - Department of Pathology, Affiliated Hospital of Taishan Medical University, 
      Tai'an, 271000, China.
FAU - Ji, Haizhen
AU  - Ji H
AD  - Department of Physiology, Taishan Medical University, Tai'an, China.
FAU - Geng, Min
AU  - Geng M
AD  - Department of Human Anatomy, Taishan Medical University, 2 Ying Sheng Dong Lu, 
      Tai'an, 271000, China.
FAU - Guo, Xingjing
AU  - Guo X
AD  - Department of Physiology, Taishan Medical University, Tai'an, China.
FAU - Wang, Zhaojin
AU  - Wang Z
AD  - Department of Human Anatomy, Taishan Medical University, 2 Ying Sheng Dong Lu, 
      Tai'an, 271000, China. zjwang@tsmc.edu.cn.
LA  - eng
GR  - 81371234/National Natural Science Foundation of China/
GR  - 2016ZRB14346/Natural Science Foundation of Shandong Province, China/
PT  - Journal Article
DEP - 20180304
PL  - Switzerland
TA  - Pathol Oncol Res
JT  - Pathology oncology research : POR
JID - 9706087
RN  - 0 (Receptors, Thrombin)
RN  - JWE1M73YZN (protease-activated receptor 4)
SB  - IM
MH  - Apoptosis/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Down-Regulation/genetics
MH  - Esophageal Neoplasms/*genetics
MH  - Esophageal Squamous Cell Carcinoma/*genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Promoter Regions, Genetic/genetics
MH  - Proteomics/methods
MH  - Receptors, Thrombin/*genetics
MH  - Up-Regulation/genetics
OTO - NOTNLM
OT  - Apoptosis
OT  - Esophageal squamous cell carcinoma
OT  - Protease-activated receptors-4
OT  - Proteomic
OT  - Tumor suppressor
EDAT- 2018/03/05 06:00
MHDA- 2019/12/20 06:00
CRDT- 2018/03/05 06:00
PHST- 2017/11/28 00:00 [received]
PHST- 2018/02/21 00:00 [accepted]
PHST- 2018/03/05 06:00 [pubmed]
PHST- 2019/12/20 06:00 [medline]
PHST- 2018/03/05 06:00 [entrez]
AID - 10.1007/s12253-018-0395-7 [pii]
AID - 10.1007/s12253-018-0395-7 [doi]
PST - ppublish
SO  - Pathol Oncol Res. 2019 Jul;25(3):937-943. doi: 10.1007/s12253-018-0395-7. Epub 
      2018 Mar 4.