PMID- 29503399
OWN - NLM
STAT- MEDLINE
DCOM- 20181030
LR  - 20181114
IS  - 1348-4400 (Electronic)
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 64
IP  - 3
DP  - 2018 Jun 22
TI  - Effect of pre-in vitro maturation with cAMP modulators on the acquisition of 
      oocyte developmental competence in cattle.
PG  - 233-241
LID - 10.1262/jrd.2018-009 [doi]
AB  - The administration of follicle-stimulating hormone (FSH) prior to oocyte 
      retrieval improves oocyte developmental competence. During bovine embryo 
      production in vitro, however, oocytes are typically derived from FSH-unprimed 
      animals. In the current study, we examined the effect of pre-in vitro maturation 
      (IVM) with cAMP modulators, also known as the second messengers of FSH, on the 
      developmental competence of oocytes derived from small antral follicles (2-4 mm) 
      of FSH-unprimed animals. Pre-IVM with N6,2'-O-dibutyryladenosine 
      3',5'-cyclicmonophosphate (dbcAMP) and 3-isobutyl-1-methylxanthine (IBMX) for 2 h 
      improved the blastocyst formation in oocytes stimulated by FSH or amphiregulin 
      (AREG). Furthermore, pre-IVM enhanced the expression of the FSH- or 
      AREG-stimulated extracellular matrix-related genes HAS2, TNFAIP6, and PTGS2, and 
      epidermal growth factor (EGF)-like peptide-related genes AREG and EREG. 
      Additionally, pre-IVM with dbcAMP and IBMX enhanced the expression of EGFR, and 
      also increased and prolonged cumulus cell-oocyte gap junctional communication. 
      The improved oocyte development observed using the pre-IVM protocol was ablated 
      by an EGF receptor phosphorylation inhibitor. These results indicate that pre-IVM 
      with cAMP modulators could contribute to the acquisition of developmental 
      competence by bovine oocytes from small antral follicles through the modulation 
      of EGF receptor signaling and oocyte-cumulus/cumulus-cumulus gap junctional 
      communication.
FAU - Sugimura, Satoshi
AU  - Sugimura S
AD  - Department of Biological Production, Institute of Agriculture, Tokyo University 
      of Agriculture and Technology, Tokyo 183-8509, Japan.
FAU - Yamanouchi, Tadayuki
AU  - Yamanouchi T
AD  - National Livestock Breeding Center, Fukushima 961-8511, Japan.
FAU - Palmerini, Maria Grazia
AU  - Palmerini MG
AD  - Department of Life, Health and Environmental Sciences, University of L'Aquila, 
      L'Aquila 67100, Italy.
FAU - Hashiyada, Yutaka
AU  - Hashiyada Y
AD  - National Livestock Breeding Center, Fukushima 961-8511, Japan.
FAU - Imai, Kei
AU  - Imai K
AD  - Department of Sustainable Agriculture, Rakuno Gakuen University, Hokkaido 
      069-8501, Japan.
FAU - Gilchrist, Robert B
AU  - Gilchrist RB
AD  - Discipline of Obstetrics & Gynaecology, School of Women's & Children's Health, 
      University of New South Wales, Sydney 2052, Australia.
LA  - eng
PT  - Journal Article
DEP - 20180302
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - E0399OZS9N (Cyclic AMP)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/*pharmacology
MH  - Animals
MH  - Bucladesine/*pharmacology
MH  - Cattle
MH  - Cyclic AMP/metabolism
MH  - Embryonic Development/*drug effects
MH  - Epidermal Growth Factor/metabolism
MH  - Female
MH  - Follicle Stimulating Hormone
MH  - In Vitro Oocyte Maturation Techniques/*veterinary
MH  - Oocyte Retrieval
MH  - Oocytes/*drug effects
MH  - Oogenesis/*drug effects
MH  - Signal Transduction/drug effects
PMC - PMC6021610
OTO - NOTNLM
OT  - Bovine
OT  - EGFR signaling
OT  - Embryo development
OT  - Gap junction
OT  - Pre-IVM
EDAT- 2018/03/06 06:00
MHDA- 2018/10/31 06:00
PMCR- 2018/06/01
CRDT- 2018/03/06 06:00
PHST- 2018/03/06 06:00 [pubmed]
PHST- 2018/10/31 06:00 [medline]
PHST- 2018/03/06 06:00 [entrez]
PHST- 2018/06/01 00:00 [pmc-release]
AID - 2018-009 [pii]
AID - 10.1262/jrd.2018-009 [doi]
PST - ppublish
SO  - J Reprod Dev. 2018 Jun 22;64(3):233-241. doi: 10.1262/jrd.2018-009. Epub 2018 Mar 
      2.