PMID- 29504577 OWN - NLM STAT- MEDLINE DCOM- 20190930 LR - 20190930 IS - 1108-7161 (Print) IS - 1108-7161 (Linking) VI - 18 IP - 1 DP - 2018 Mar 1 TI - Expression of BDNF in dorsal root ganglion of rats with bone cancer pain and its effect on pain behavior. PG - 42-46 AB - OBJECTIVE: To detect the expression of brain-derived neurotrophic factor (BDNF) in dorsal root ganglion (DRG) of rats with bone cancer pain, and to explore the effects of BDNF and anti-BDNF on pain behavior. METHODS: 40 SD rats were randomly divided into Sham group and Cancer group (n=20). Rats in Sham were injected with PBS into the tibia, while rats in Cancer group were injected with Walker 256 cells to construct rat tibial bone cancer pain model. Rats in Cancer group were further divided into physiological saline (NS) group and anti-BDNF group (n=10) to observe the effects of anti-BDNF on pain behavior in rats with bone cancer pain. RESULTS: Expression level of BDNF and its receptor in DRG of Cancer group was significantly higher than that of Sham group at 3 weeks after inoculation (p⟨0.05). The expression level of BDNF mRNA in the Cancer group was significantly higher than that in the Sham group at 5d, 10d and 20d (p⟨0.05). Hindpaw withdrawal latency was significantly shorter in Cancer group than in Sham group (p⟨0.05). Hindpaw withdrawal mechanical threshold was significantly lower in Cancer group than in Sham group (p⟨0.05). Hindpaw withdrawal latency was significantly longer and hindpaw withdrawal mechanical threshold was significantly higher in anti-BDNF group than in NS group after the use of anti-BDNF (p⟨0.05). CONCLUSION: BDNF can aggravate bone pain in rats, and anti-BDNF has obvious antinociceptive function in bone cancer pain. FAU - Huang, Youqing AU - Huang Y AD - Department of Pain, The Second Affiliated Hospital of Kunming Medical University, No.374, Dianmian Road, Kuming, Yunnan, 650101, P.R. China. LA - eng PT - Journal Article PL - Greece TA - J Musculoskelet Neuronal Interact JT - Journal of musculoskeletal & neuronal interactions JID - 101084496 RN - 0 (Brain-Derived Neurotrophic Factor) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Animals MH - Behavior, Animal/*physiology MH - Bone Neoplasms/*metabolism MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Cancer Pain/*metabolism MH - Female MH - Ganglia, Spinal/*metabolism MH - Pain Measurement MH - Pain Threshold/physiology MH - Rats MH - Rats, Sprague-Dawley MH - Receptor, trkB/metabolism PMC - PMC5881127 COIS- The author has no conflict of interest. EDAT- 2018/03/06 06:00 MHDA- 2019/10/01 06:00 PMCR- 2018/03/01 CRDT- 2018/03/06 06:00 PHST- 2018/03/06 06:00 [entrez] PHST- 2018/03/06 06:00 [pubmed] PHST- 2019/10/01 06:00 [medline] PHST- 2018/03/01 00:00 [pmc-release] AID - JMNI-18-042 [pii] PST - ppublish SO - J Musculoskelet Neuronal Interact. 2018 Mar 1;18(1):42-46.