PMID- 29504750
OWN - NLM
STAT- MEDLINE
DCOM- 20190205
LR  - 20190215
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 90
IP  - 6
DP  - 2018 Mar 20
TI  - FT-IR Spectroscopic Imaging of Endothelial Cells Response to Tumor Necrosis 
      Factor-alpha: To Follow Markers of Inflammation Using Standard and High-Magnification 
      Resolution.
PG  - 3727-3736
LID - 10.1021/acs.analchem.7b03089 [doi]
AB  - Two endothelial cell lines were selected as models to investigate an effect of 
      incubation with cytokine tumor necrosis factor type alpha (TNF-alpha) using Fourier 
      transform infrared (FT-IR) imaging spectroscopy. Both cell lines are often used 
      in laboratories and are typical lung vascular endothelial cells (HMLVEC) derived 
      from the fusion of umbilical vein endothelial cells with lung adenocarcinoma 
      cells (EA.hy926). This study was focused on alteration of spectral changes 
      accompanying inflammation at the cellular level by applying two resolution 
      systems of FT-IR microscopy. The standard approach, with a pixel size of ca. 5.5 
      mum(2), determined the inflammatory state of the whole cell, while a 
      high-magnification resolution (pixel size of ca. 1.1 mum(2)) provided information 
      at the subcellular level. Importantly, the analysis of IR spectra recorded with 
      different modes produced similar results overall and yielded unambiguous 
      classification of inflamed cells. Generally, the most significant changes in the 
      cells under the influence of TNF-alpha are related with lipids-their composition and 
      concentration; however, segregation of cells into subcellular compartments 
      provided an additional insight into proteins and nucleic acids related events. 
      The observed spectral alterations are specific for the type of endothelial cell 
      line.
FAU - Wiercigroch, Ewelina
AU  - Wiercigroch E
AD  - Faculty of Chemistry , Jagiellonian University , Gronostajowa 2 , 30-387 Krakow , 
      Poland.
FAU - Staniszewska-Slezak, Emilia
AU  - Staniszewska-Slezak E
AD  - Faculty of Chemistry , Jagiellonian University , Gronostajowa 2 , 30-387 Krakow , 
      Poland.
FAU - Szkaradek, Kinga
AU  - Szkaradek K
AD  - Jagiellonian Centre for Experimental Therapeutics , Jagiellonian University , 
      Bobrzynskiego 14 , 30-348 Krakow , Poland.
FAU - Wojcik, Tomasz
AU  - Wojcik T
AD  - Jagiellonian Centre for Experimental Therapeutics , Jagiellonian University , 
      Bobrzynskiego 14 , 30-348 Krakow , Poland.
FAU - Ozaki, Yukihiro
AU  - Ozaki Y
AUID- ORCID: 0000-0002-4479-4004
AD  - Department of Chemistry , School of Science and Technology, Kwansei Gakuin 
      University , Gakuen 2-1 , Sanda , Hyogo 669-1337 , Japan.
FAU - Baranska, Malgorzata
AU  - Baranska M
AD  - Faculty of Chemistry , Jagiellonian University , Gronostajowa 2 , 30-387 Krakow , 
      Poland.
AD  - Jagiellonian Centre for Experimental Therapeutics , Jagiellonian University , 
      Bobrzynskiego 14 , 30-348 Krakow , Poland.
FAU - Malek, Kamilla
AU  - Malek K
AUID- ORCID: 0000-0003-0582-2743
AD  - Faculty of Chemistry , Jagiellonian University , Gronostajowa 2 , 30-387 Krakow , 
      Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180309
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Biomarkers)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Biomarkers/analysis
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Endothelial Cells/*immunology
MH  - Humans
MH  - Inflammation/*diagnosis/immunology
MH  - Spectroscopy, Fourier Transform Infrared/*methods
MH  - Tumor Necrosis Factor-alpha/*immunology
EDAT- 2018/03/06 06:00
MHDA- 2019/02/06 06:00
CRDT- 2018/03/06 06:00
PHST- 2018/03/06 06:00 [pubmed]
PHST- 2019/02/06 06:00 [medline]
PHST- 2018/03/06 06:00 [entrez]
AID - 10.1021/acs.analchem.7b03089 [doi]
PST - ppublish
SO  - Anal Chem. 2018 Mar 20;90(6):3727-3736. doi: 10.1021/acs.analchem.7b03089. Epub 
      2018 Mar 9.