PMID- 29505314
OWN - NLM
STAT- MEDLINE
DCOM- 20190516
LR  - 20190516
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 43
IP  - 6
DP  - 2018 Jun
TI  - Neuroprotection of Transplanting Human Umbilical Cord Mesenchymal Stem Cells in a 
      Microbead Induced Ocular Hypertension Rat Model.
PG  - 810-820
LID - 10.1080/02713683.2018.1440604 [doi]
AB  - PURPOSE: The purpose of this study is to investigate the potential therapeutic 
      benefits of intravitreally transplanted human umbilical cord mesenchymal stem 
      cells (UC-MSCs) in an animal model of microbead-injection-induced ocular 
      hypertension (OHT). METHODS: UC-MSCs were isolated from human umbilical cords and 
      then cultured. The OHT model was induced via intracameral injection of 
      polystyrene microbeads in Sprague-Dawley adult rat eyes. Fifty-four healthy adult 
      rats were randomly divided into three groups: normal control, OHT model treated 
      with intravitreal transplantation of UC-MSCs, or phosphate-buffered saline (PBS). 
      Two days after OHT was induced, either 5 microl 10(5) UC-MSCs suspension or PBS was 
      injected into the vitreous cavity of rats. UC-MSCs localization and integration 
      were examined via immunohistochemistry. Neuroprotection was quantified by 
      counting retinal ganglion cells (RGCs) and axons 2 weeks following 
      transplantation. The expression levels of glial-derived neurotrophic factor 
      (GDNF), brain-derived neurotrophic factor (BDNF), and glial fibrillary acidic 
      protein (GFAP) were assessed via immunohistochemistry and Western blot. 
      Functional recovery was assessed 2 weeks after transplantation via scotopic 
      threshold response (STR) electroretinography. RESULTS: Elevated IOP levels were 
      sustained at least 3 weeks after intracameral microbead injection and the number 
      of beta-III-tubulin(+) RGCs significantly declined compared to PBS-injected eyes. 
      UC-MSCs survived for at least 2 weeks after intravitreal transplantation and 
      predominantly located in the vitreous cavity. A fraction of cells migrated into 
      the ganglion cell layer of host retina, but without differentiation. Intravitreal 
      UC-MSC transplantation resulted in increased number of RGCs, axons, and increased 
      expression of GDNF and BDNF but decreased expression of GFAP. Intravitreal 
      delivery of UC-MSCs significantly improved the recovery of the positive STR. 
      CONCLUSIONS: Intravitreal transplantation of UC-MSCs revealed the neuroprotection 
      in the microbead-injection induced OHT. The effects could be related to the 
      secretion of tropic factors (BDNF and GDNF) and the modulation of glial cell 
      activation.
FAU - Ji, Shangli
AU  - Ji S
AD  - a Aier School of Ophthalmology , Central South University , Changsha , Hunan , 
      China.
FAU - Lin, Saiyue
AU  - Lin S
AD  - b Department of Anatomy and Neurobiology, Xiangya School of Medicine , Central 
      South University , Changsha , Hunan , China.
FAU - Chen, Jiansu
AU  - Chen J
AD  - a Aier School of Ophthalmology , Central South University , Changsha , Hunan , 
      China.
FAU - Huang, Xinping
AU  - Huang X
AD  - c Department of Biology , ShenzhenHornetcorn Biotechnology Co., Ltd , Shenzhen , 
      Guangdong , China.
FAU - Wei, Chih-Chang
AU  - Wei CC
AD  - c Department of Biology , ShenzhenHornetcorn Biotechnology Co., Ltd , Shenzhen , 
      Guangdong , China.
FAU - Li, Zhiyuan
AU  - Li Z
AD  - b Department of Anatomy and Neurobiology, Xiangya School of Medicine , Central 
      South University , Changsha , Hunan , China.
FAU - Tang, Shibo
AU  - Tang S
AD  - a Aier School of Ophthalmology , Central South University , Changsha , Hunan , 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180305
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Cell Differentiation
MH  - Disease Models, Animal
MH  - Intraocular Pressure/*physiology
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*cytology
MH  - Microspheres
MH  - *Neuroprotection
MH  - Ocular Hypertension/diagnosis/physiopathology/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retinal Ganglion Cells/pathology
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - UC-MSCs
OT  - Umbilical cord mesenchymal stem cells
OT  - high intraocular pressure
OT  - neuroprotection
OT  - ocular hypertension
OT  - retinal ganglion cells
EDAT- 2018/03/06 06:00
MHDA- 2019/05/17 06:00
CRDT- 2018/03/06 06:00
PHST- 2018/03/06 06:00 [pubmed]
PHST- 2019/05/17 06:00 [medline]
PHST- 2018/03/06 06:00 [entrez]
AID - 10.1080/02713683.2018.1440604 [doi]
PST - ppublish
SO  - Curr Eye Res. 2018 Jun;43(6):810-820. doi: 10.1080/02713683.2018.1440604. Epub 
      2018 Mar 5.