PMID- 29505523
OWN - NLM
STAT- MEDLINE
DCOM- 20180309
LR  - 20221207
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Print)
IS  - 0025-7974 (Linking)
VI  - 97
IP  - 1
DP  - 2018 Jan
TI  - Aberrations and clinical significance of BRAF in malignant melanoma: A series of 
      60 cases in Chinese Uyghur.
PG  - e9509
LID - 10.1097/MD.0000000000009509 [doi]
LID - e9509
AB  - Malignant melanoma (MM) is a highly malignant melanocytic tumor, it occurs mostly 
      in the skin, the mucous membrane close to the skin, but also in the tunicae 
      rhagoides and the pia mater. The Uyghur is the largest ethnic group living in the 
      Xinjiang Uyghur Autonomous Region of China, accounting for 46% of the total 
      population of 20 million. Large-scale studies on MMs in Asian countries are 
      limited. This study aimed to investigate BRAF mRNA expression and mutations in 
      Chinese Uyghur patients with MMs and to identify the clinical features associated 
      with these parameters.Formalin-fixed, paraffin wax-embedded tumor sections from 
      60 MMs were analyzed for BRAF expression using reverse transcription polymerase 
      chain reaction (RT-PCR). Exons 11 and 15 of BRAF were analyzed for the presence 
      of mutations using PCR and DNA sequencing. Sixty MMs were followed by mobile 
      phone for survival analysis.BRAF mRNA expression was higher in MMs than in 
      pigmented moles and normal skin tissues. Fourteen of 60 MMs had BRAF mutations. 
      The frequency of BRAF mutations was significantly higher in patients younger than 
      60 years (10/28, 4/32, P = .02). A significant difference was observed in the 
      frequency of BRAF mutations among specimens of mucosal, acral, chronic 
      sun-induced damage (CSD), and non-CSD MMs (2/10, 3/19, 8/25, 1/6, P = .002). No 
      significant association was found among BRAF mutations, sex, ulceration, or lymph 
      node metastasis. MMs lymph node metastasis (hazard ratio 2.54 [95% confidence 
      interval 1.062 - 6.066], P = .01) affected survival.This study indicated that 
      BRAF mutations and expression might serve as independent adverse prognostic 
      factors in melanoma.
CI  - Copyright (c) 2017 The Authors. Published by Wolters Kluwer Health, Inc. All rights 
      reserved.
FAU - Kang, Xiaojing
AU  - Kang X
AD  - Department of Dermatology Department of Pathology, People's Hospital of Xinjiang 
      Uyghur Autonomous Region, Urumqi, Xinjiang, China.
FAU - Zeng, Ying
AU  - Zeng Y
FAU - Liang, Junqin
AU  - Liang J
FAU - Li, Jing
AU  - Li J
FAU - Ren, Danyang
AU  - Ren D
FAU - Chai, Li
AU  - Chai L
FAU - Sun, Zhenzhu
AU  - Sun Z
FAU - Yu, Shirong
AU  - Yu S
FAU - Wu, Xiujuan
AU  - Wu X
FAU - Han, Wen
AU  - Han W
FAU - Wang, Weijia
AU  - Wang W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - EC 2.7.11.1 (BRAF protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins B-raf)
SB  - IM
MH  - Aged
MH  - Asian People/genetics
MH  - China/epidemiology
MH  - Female
MH  - Humans
MH  - Male
MH  - Melanoma/*genetics/metabolism/mortality
MH  - Middle Aged
MH  - Mutation
MH  - Proto-Oncogene Proteins B-raf/*genetics/metabolism
MH  - Skin Neoplasms/*genetics/metabolism/mortality
MH  - Survival Analysis
PMC - PMC5943096
COIS- The authors state that they have no conflicts of interest.
EDAT- 2018/03/06 06:00
MHDA- 2018/03/10 06:00
PMCR- 2018/01/05
CRDT- 2018/03/06 06:00
PHST- 2018/03/06 06:00 [entrez]
PHST- 2018/03/06 06:00 [pubmed]
PHST- 2018/03/10 06:00 [medline]
PHST- 2018/01/05 00:00 [pmc-release]
AID - 00005792-201801050-00020 [pii]
AID - MD-D-17-03014 [pii]
AID - 10.1097/MD.0000000000009509 [doi]
PST - ppublish
SO  - Medicine (Baltimore). 2018 Jan;97(1):e9509. doi: 10.1097/MD.0000000000009509.