PMID- 29505785
OWN - NLM
STAT- MEDLINE
DCOM- 20190204
LR  - 20190215
IS  - 1873-7064 (Electronic)
IS  - 0028-3908 (Linking)
VI  - 135
DP  - 2018 Jun
TI  - Striatal GluN2B involved in motor skill learning and stimulus-response learning.
PG  - 73-85
LID - S0028-3908(18)30103-5 [pii]
LID - 10.1016/j.neuropharm.2018.03.002 [doi]
AB  - Although the striatum has a well-documented role in procedural learning and 
      memory, the underlying mechanisms remain poorly understood. GluN2B subunit is 
      abundantly expressed in striatum, however, the function of striatal GluN2B 
      subunit in striatum-related learning is also unclear. In the present study, using 
      transgenic mice with forebrain-specific overexpression of the GluN2B subunit, we 
      observed that up-regulation of GluN2B subunit expression results in enhanced 
      dorsal striatum-related motor skill learning and stimulus-response (S-R) learning 
      as well as cortico-dorsomedial striatal (cortico-DMS) long-term potentiation 
      (LTP). Consistent with the above results, we also found that GluN2B transgenic 
      mice exhibited a remarkable increase in N-methyl-d-aspartic acid receptor (NMDAR) 
      mediated currents in the dorsomedial striatum. In addition, in order to further 
      verify that striatal GluN2B is involved in the dorsal striatum-related cognitive 
      function, lentivirus-mediated short hairpin RNA (shRNA) was used to silence the 
      expression of GluN2B gene in the dorsomedial striatum. As a consequence of 
      down-regulation of dorsomedial striatal GluN2B subunit expression, defective 
      motor skill learning and S-R learning were observed in the GluN2B-shRNA mice. 
      Furthermore, the impaired cortico-DMS LTP, as well as decreased NMDAR mediated 
      currents in the dorsomedial striatum were also detected. Taken together, our 
      findings demonstrate for the first time that striatal GluN2B subunit plays an 
      important role in the dorsal striatum-related motor skill learning and S-R 
      learning and provide further evidence that the cortico-DMS LTP underlies dorsal 
      striatum-related motor skill learning and S-R learning.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Duan, Yanhong
AU  - Duan Y
AD  - Key Laboratory of Brain Functional Genomics, Ministry of Education, Shanghai Key 
      Laboratory of Brain Functional Genomics, School of Life Sciences, East China 
      Normal University, Shanghai, China.
FAU - Wang, Qi
AU  - Wang Q
AD  - Key Laboratory of Brain Functional Genomics, Ministry of Education, Shanghai Key 
      Laboratory of Brain Functional Genomics, School of Life Sciences, East China 
      Normal University, Shanghai, China.
FAU - Zeng, Qingwen
AU  - Zeng Q
AD  - Key Laboratory of Brain Functional Genomics, Ministry of Education, Shanghai Key 
      Laboratory of Brain Functional Genomics, School of Life Sciences, East China 
      Normal University, Shanghai, China.
FAU - Wang, Jiayue
AU  - Wang J
AD  - Key Laboratory of Brain Functional Genomics, Ministry of Education, Shanghai Key 
      Laboratory of Brain Functional Genomics, School of Life Sciences, East China 
      Normal University, Shanghai, China.
FAU - Chen, Zhenzhen
AU  - Chen Z
AD  - Key Laboratory of Brain Functional Genomics, Ministry of Education, Shanghai Key 
      Laboratory of Brain Functional Genomics, School of Life Sciences, East China 
      Normal University, Shanghai, China.
FAU - Xu, Meichen
AU  - Xu M
AD  - Key Laboratory of Brain Functional Genomics, Ministry of Education, Shanghai Key 
      Laboratory of Brain Functional Genomics, School of Life Sciences, East China 
      Normal University, Shanghai, China.
FAU - Duan, Yale
AU  - Duan Y
AD  - Key Laboratory of Brain Functional Genomics, Ministry of Education, Shanghai Key 
      Laboratory of Brain Functional Genomics, School of Life Sciences, East China 
      Normal University, Shanghai, China.
FAU - Zhao, Zheng
AU  - Zhao Z
AD  - Key Laboratory of Brain Functional Genomics, Ministry of Education, Shanghai Key 
      Laboratory of Brain Functional Genomics, School of Life Sciences, East China 
      Normal University, Shanghai, China.
FAU - Xue, Qingsheng
AU  - Xue Q
AD  - Department of Anesthesiology, Ruijin Hospital, Shanghai Jiaotong University 
      School of Medicine, Shanghai, China. Electronic address: xqs11260@rjh.com.cn.
FAU - Cao, Xiaohua
AU  - Cao X
AD  - Key Laboratory of Brain Functional Genomics, Ministry of Education, Shanghai Key 
      Laboratory of Brain Functional Genomics, School of Life Sciences, East China 
      Normal University, Shanghai, China. Electronic address: xhcao@brain.ecnu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180302
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Animals
MH  - Corpus Striatum/physiology
MH  - Down-Regulation
MH  - Learning/*physiology
MH  - Long-Term Potentiation/physiology
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Motor Skills/*physiology
MH  - Neostriatum/metabolism
MH  - RNA, Small Interfering/pharmacology
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & 
      inhibitors/biosynthesis/genetics/*physiology
MH  - Up-Regulation
OTO - NOTNLM
OT  - Dorsal striatum
OT  - GluN2B
OT  - LTP
OT  - Motor skill learning
OT  - NMDAR
OT  - S-R learning
EDAT- 2018/03/06 06:00
MHDA- 2019/02/05 06:00
CRDT- 2018/03/06 06:00
PHST- 2017/11/03 00:00 [received]
PHST- 2018/02/27 00:00 [revised]
PHST- 2018/03/01 00:00 [accepted]
PHST- 2018/03/06 06:00 [pubmed]
PHST- 2019/02/05 06:00 [medline]
PHST- 2018/03/06 06:00 [entrez]
AID - S0028-3908(18)30103-5 [pii]
AID - 10.1016/j.neuropharm.2018.03.002 [doi]
PST - ppublish
SO  - Neuropharmacology. 2018 Jun;135:73-85. doi: 10.1016/j.neuropharm.2018.03.002. 
      Epub 2018 Mar 2.