PMID- 29506120 OWN - NLM STAT- MEDLINE DCOM- 20190114 LR - 20211204 IS - 1460-2350 (Electronic) IS - 0268-1161 (Linking) VI - 33 IP - 4 DP - 2018 Apr 1 TI - Granulosa cells from human primordial and primary follicles show differential global gene expression profiles. PG - 666-679 LID - 10.1093/humrep/dey011 [doi] AB - STUDY QUESTION: Can novel genetic candidates involved in follicle dormancy, activation and integrity be identified from transcriptomic profiles of isolated granulosa cells from human primordial and primary follicles? SUMMARY ANSWER: The granulosa cell compartment of the human primordial and primary follicle was extensively enriched in signal transducer and activator of transcription 3 (STAT3) and cAMP-response element binding protein (CREB) signalling, and several other putative signalling pathways that may also be mediators of follicle growth and development were identified. WHAT IS KNOWN ALREADY: Mechanistic target of rapamycin kinase (mTOR) signalling and the factors Forkhead Box L2 (FOXL2) and KIT proto-oncogene receptor tyrosine kinase (KITL) may be involved in defining the early steps of mammalian follicular recruitment through complex bidirectional signalling between the oocyte and granulosa cells. cAMP/protein kinase K (PKA)/CREB signalling is a feature of FSH-induced regulation of granulosa cell steroidogenesis that is essential to normal human fertility. STUDY DESIGN, SIZE, DURATION: A class comparison study was carried out on primordial follicles (n = 539 follicles) and primary follicles (n = 261) follicles) donated by three women having ovarian tissue cryopreserved before chemotherapy. PARTICIPANTS/MATERIALS, SETTING, METHODS: RNA samples from isolates of laser capture micro-dissected oocytes and follicles from the primordial and primary stage, respectively, were sequenced on the HiSeq Illumina platform. Data mapping, quality control, filtering, FPKM (fragments per kilobase of exon per million) normalization and comparisons were performed. The granulosa cell contribution in whole follicle isolates was extracted in silico. Modelling of complex biological systems was performed using Ingenuity Pathway Analysis (IPA). For validation of transcriptomic findings, we performed quantitative RT-PCR of selected candidate genes. Furthermore, we interrogated the in situ localization of selected corresponding proteins using immunofluorescence. MAIN RESULTS AND THE ROLE OF CHANCE: Our differentially expressed gene analysis revealed a number of transcripts in the granulosa cells to be significantly down- (736 genes) or up- (294 genes) regulated during the human primordial-to-primary follicle transition. The IPA analysis revealed enriched canonical signalling pathways not previously associated with granulosa cells from human primordial and primary follicles. Immunofluorescent staining of human ovarian tissue explored the intra-ovarian localization of FOG2, and FOXL2, which revealed the presence of forkhead box L2 (FOXL2) in both oocytes and granulosa cells in primary follicles, with a more enriched staining in the granulosa cells in primary follicles. Friend of GATA 2 (FOG2) stained strongly in oocytes in primordial follicles, with a shift towards granulosa cell as follicle stage advanced. LARGE SCALE DATA: http://users-birc.au.dk/biopv/published_data/ernst_et_al_GC_2017/. LIMITATIONS REASONS FOR CAUTION: This is a descriptive study, and no functional assays were employed. The study was based on a limited number of patients, and it is acknowledged that natural biological variance exists in human samples. Strict filters were applied to accommodate the in silico extraction of the granulosa cell contribution. In support of this, quantitative RT-PCR was used to confirm selected candidate genes, and immunofluorescent staining was employed to interrogate the intra-ovarian distribution of selected corresponding proteins. Moreover, it is unknown whether the primordial follicles analysed represent those still in the resting pool, or those from the cohort that have entered the growing pool. WIDER IMPLICATIONS OF THE FINDINGS: We present, for the first time, a detailed description of global gene activity in the human granulosa cell compartment of primordial and primary follicles. These results may be utilized in the development of novel clinical treatment strategies aimed at improving granulosa cell function. STUDY FUNDING/COMPETING INTEREST(S): E.H.E. was supported by the Health Faculty, Aarhus University and Kong Christian Den Tiendes Fond. K.L.H. was supported by a grant from Fondens til Laegevidenskabens Fremme and Kong Christian Den Tiendes Fond. No authors have competing interests to declare. FAU - Ernst, E H AU - Ernst EH AD - Department of Biomedicine, Aarhus University, Wilhelm Meyers Alle 4, DK-8000 Aarhus C, Denmark. FAU - Franks, S AU - Franks S AD - Institute of Reproductive and Developmental Biology, Imperial College London, South Kensington Campus, London SW7 2AZ, UK. FAU - Hardy, K AU - Hardy K AD - Institute of Reproductive and Developmental Biology, Imperial College London, South Kensington Campus, London SW7 2AZ, UK. FAU - Villesen, P AU - Villesen P AD - Bioinformatic Research Centre (BiRC), Aarhus University, C.F. Mollers Alle 8, DK-8000 Aarhus C, Denmark. AD - Department of Clinical Medicine, Aarhus University, Wilhelm Meyers Alle 4, DK-8000 Aarhus C, Denmark. FAU - Lykke-Hartmann, K AU - Lykke-Hartmann K AD - Department of Biomedicine, Aarhus University, Wilhelm Meyers Alle 4, DK-8000 Aarhus C, Denmark. AD - Department of Clinical Medicine, Aarhus University, Wilhelm Meyers Alle 4, DK-8000 Aarhus C, Denmark. AD - Department of Clinical Genetics, Aarhus University Hospital, Brendstrupgardsvej 21, DK-8200 Aarhus N, Denmark. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Hum Reprod JT - Human reproduction (Oxford, England) JID - 8701199 RN - 0 (MAS1 protein, human) RN - 0 (Proto-Oncogene Mas) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) SB - IM MH - Female MH - Gene Expression Profiling MH - Granulosa Cells/*metabolism MH - Humans MH - Ovarian Follicle/*metabolism MH - Proto-Oncogene Mas MH - Signal Transduction/genetics MH - TOR Serine-Threonine Kinases/genetics/metabolism MH - *Transcriptome EDAT- 2018/03/06 06:00 MHDA- 2019/01/15 06:00 CRDT- 2018/03/06 06:00 PHST- 2017/08/03 00:00 [received] PHST- 2018/01/12 00:00 [accepted] PHST- 2018/03/06 06:00 [pubmed] PHST- 2019/01/15 06:00 [medline] PHST- 2018/03/06 06:00 [entrez] AID - 4915549 [pii] AID - 10.1093/humrep/dey011 [doi] PST - ppublish SO - Hum Reprod. 2018 Apr 1;33(4):666-679. doi: 10.1093/humrep/dey011.