PMID- 29509139 OWN - NLM STAT- MEDLINE DCOM- 20180619 LR - 20180620 IS - 0036-7281 (Print) IS - 0036-7281 (Linking) VI - 160 IP - 3 DP - 2018 Mar TI - Determination of reference intervals and comparison of venous blood gas parameters using a standard and nonstandard collection method in 51 dogs. PG - 163-170 LID - 10.17236/sat00150 [doi] AB - The aim of this study was to determine reference intervals (RI) for venous blood parameters determined with the RAPIDPoint 500 (RP500) blood gas analyzer using blood gas syringes (BGS) and to determine whether immediate analysis of venous blood collected into lithium heparin (LH) tubes can replace anaerobic blood sampling into BGS. The null hypothesis was that canine venous blood samples collected in BGS and in LH tubes are comparable. Jugular blood was collected from 51 healthy dogs into a BGS and a LH tube. The BGS was immediately analyzed followed by the LH tube. The RI were calculated from BGS results. The BGS and LH tubes results were compared using paired t-test or Wilcoxon matched-pairs signed-rank test and Bland-Altman analysis. To assess clinical relevance, the bias between BGS and LH tubes was compared with the allowable total error (TEa). Values derived from LH tubes showed no significant difference for standard bicarbonate (HCO3std), whole blood base excess (BE B), Na, K, Cl, glucose and hemoglobin (tHb). The pH, partial pressure of carbon dioxide and oxygen, actual bicarbonate, extracellular base excess, ionized Ca, anion gap and lactate were significantly (p. FAU - Bachmann, K AU - Bachmann K AD - Department for Small Animals, Vetsuisse Faculty, University of Zurich. FAU - Kutter, A AU - Kutter A AD - Section of Anaesthesiology, Equine Department, Vetsuisse Faculty, University of Zurich. FAU - Jud Schefer, R S AU - Jud Schefer RS AD - Department for Small Animals, Vetsuisse Faculty, University of Zurich. FAU - Sigrist, N AU - Sigrist N AD - Department for Small Animals, Vetsuisse Faculty, University of Zurich. LA - eng PT - Journal Article TT - Bestimmung der Referenzintervalle von venosen Blutgasparametern bei 51 Hunden und Vergleich der standardisierten mit einer nicht-standardisierten Untersuchungsmethode. PL - Switzerland TA - Schweiz Arch Tierheilkd JT - Schweizer Archiv fur Tierheilkunde JID - 0424247 SB - IM MH - Animals MH - Blood Chemical Analysis/methods/standards/veterinary MH - Blood Gas Analysis/methods/standards/*veterinary MH - Blood Specimen Collection/instrumentation/*veterinary MH - Dogs/*blood MH - Female MH - Male MH - Reference Values OTO - NOTNLM OT - Hund OT - Laktat OT - Saure-Base Haushalt OT - acid base OT - canine OT - ionisiertes Kalzium OT - ionized calcium OT - lactate EDAT- 2018/03/07 06:00 MHDA- 2018/06/21 06:00 CRDT- 2018/03/07 06:00 PHST- 2018/03/07 06:00 [entrez] PHST- 2018/03/07 06:00 [pubmed] PHST- 2018/06/21 06:00 [medline] AID - 10.17236/sat00150 [doi] PST - ppublish SO - Schweiz Arch Tierheilkd. 2018 Mar;160(3):163-170. doi: 10.17236/sat00150.